脂质体的

Methods: Genes of MCP and DAF were connected by 10aa coding sequence of enriching Gly and Ser. Then insert into pcDAN3.1 vector, constituting pcDNA-MD3 expression plasmid of hMCP-DAF fusion gene. The genes were transformed by liposome into the pig endothelial cell; human serum handles masculine cloning, identify the function that restrain breaking of person alexin.

将MCP、DAF两个基因以富含Gly和Ser的10aa编码序列作铰链串接，并接入表达载体pcDNA 3.1中，构成含hMCP-DAF的真核表达质粒(pcDNA-MD3)；以脂质体转染猪内皮细胞，人血清处理阳性克隆细胞，鉴定抑制人补体溶破的功能；应用显微注射将线性基因导入猪受精卵；PCR检测导入基因整合率。

Anisodamine causes interdigitation in DPPG, whose phospholipid molecules insert into the opposite lipid layer with the ends of the acyl chains near to the 5th carbons of the opposing lipid molecules, whereas anisodamine can not cause interdigitation in DPPC existed generally in membranes.

山莨菪碱诱导DPPG脂质体交插结构，其脂酰链末端插到对面分子层脂酰链第五个碳原子的位置，而生物膜中普遍存在的DPPC不能被山莨菪碱诱导形成交插相，但DPPG／DPPC混合物则能形成交插相，即伴随DPPG的交插，DPPC分子也发生交插。

The expression of CD59 on the cell membrane was tested by cell immunohistochemistry and flow cytometry. Results pALTER plasmid containing CD59 was cut with restriction enzymes and a 496 bp fragment obtained by electrophoresis, which was complete conformity with the insert.

将两种含有不同突变体的人CD59全长cDNA序列重组pALTER质粒，应用阳离子脂质体导入法与pcDNA共转染CHO细胞，用G418筛选阳性克隆，应用细胞免疫组化及流式细胞仪检测CD59在CHO细胞表面的表达。

This paper summarizes the development of proplastid,lay stress on the synthesis relativity between plastid and lipid.

阐述了能源植物前质体的发育，着重介绍了质体与脂类合成相关性等方面的研究进展。

Methods Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue.

以实验性高脂血及轻度氧化LDL局部保留灌注的家兔股动脉为模型，采用DOTAP阳离子脂质体包裹LNCX-anti-MCP-1质粒，将反义MCP-1 cDNA定位导入家兔股动脉鞘及周围组织，以原位杂交、 Northern杂交及狭缝杂交法观察反义MCP-1基因的表达，应用扫描电镜观察单核细胞在动脉内膜粘附的情况。

All the transfected and un-transfected rMSCs were attached to Allo-DBMs. These new biomaterials were implanted in muscle bags and segmental radius defects of the New Zealand white rabbits, and some controlled material groups were established for comparison. All the biomaterials and the controlled materials were assessed by gross observation, radiographical and histological methods.

取兔自体骨髓基质细胞培养扩增，用脂质体介导的方法转染人骨发生蛋白2基因，将转染和未转染人骨发生蛋白2基因的自体骨髓基质细胞附和于异体兔脱钙骨基质形成新的生物植骨材料，连同空白及单纯脱钙骨基质对照组植入兔前肢肌袋和桡骨缺损。2、4、6、8周分别取材进行大体，放射线和病理观察。

The effect of aluminum ions on the Fe2+-initiated lipid peroxidation in liposomes was studied by chemiluminescence, TBARS, conjugate dienes assays and ESR spectroscopy.

利用化学发光、TBA 反应与测量共轭二烯的方法观测了Al3＋对Fe2＋启动的卵磷脂脂质体脂质过氧化的影响。

Wistar rats were fed with a high lipid diet supplemented with living or thermal death bacteria of Lactobacillus acidophilus MG2-1 which was isolated from koumiss in Mongolia and was of good ability of acid tolerance and decreasing the level of cholesterol in vitro.

利用从蒙古国酸马奶中分离鉴定，并通过耐酸性及体外降胆固醇能力测试筛选获得的L.acidophilus MG2-1菌制备成活菌体和热致死菌体制剂与高脂饲料同时灌喂Wistar系大鼠，研究探讨了对其血清脂质代谢的影响。

Results showed that the fluidity of the liposomal membrane after coating with the two macromolecules had no obvious changes.

可见，胶原蛋白、壳聚糖包覆脂质体能够在基本不干扰脂质体膜流动性的情况下，明显地提高其体外的稳定性

The density of TILs and cytokine level in liver and plasma were determined by immunohistochemical staining and ELISA, respectively.

静脉注射SEA脂体，ELSIA法检测小鼠血浆中TNF-α、IFN-γ的水平；观察SEA脂质体对新西兰大白兔血压、呼吸及体温的影响。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。