脂质体

The peroxisome proliferator-activated receptors play an important role in adipocyte differentiation and fatty acid metabolism, activation of PPAR may induce the differentiation of adipocyte,influence the expression of genes involved in lipogenesis and fatty acid oxidation,eventually lessen ectopic fat accumulation and improve the impaired insulin signaling,so the medicine which targets to the control point of FFA metabolism would be hopeful in the treatment of a cluster of metabolic abnormalities resulted from insulin resistance.

过氧化物酶体增殖物激活受体在脂肪细胞分化、脂肪酸代谢中起重要作用，PPAR及其所调控基因的激活可促进脂肪细胞分化、减少脂质产生、增加脂肪酸氧化，从而减少脂质的异位沉积，进一步改善损伤的胰岛素信号转导通路，逆转胰岛素抵抗。针对脂肪酸合成及氧化的关键调控点的药物可能是今后治疗胰岛素抵抗引发的一系列代谢异常的新的靶点。关键词过氧化物酶体增殖物激活受体；脂肪酸代谢；胰岛素抵抗

It was suggested that the pharmacodynamics and therapeutic function of Qizhi complex prescription to fatty liver were: 1. Adjust lipometabolism: reducing TC and TG; 2. Decreasing fatty degeneration of liver cell; 3. Promoting the convalescence of hepatocellular function; 4. Anti-lipid peroxide: increasing the activation of peroxidase, decreasing the content of products of lipid peroxide. 5. Restraining Kupffer cell function of fatty liver rat; 6. Improving fluidity of hepatocellular microsome and mitochondria.

芪枳合剂治疗脂肪肝的效应及药效学机制为： 1、调整脂质代谢：降低血清甘油三脂和胆固醇； 2、减轻肝细胞脂肪变性； 3、促进肝细胞功能恢复，有效治疗脂肪性肝炎； 4、抗脂质过氧化：提高抗氧化酶活性、降低脂质过氧化产物含量； 5、抑制脂肪肝大鼠Kupffer细胞功能； 6、改善肝细胞微粒体、线粒体膜流动性。

In present study, we observed effect of taurine on liver lipid metabolism in rat, an animal capable of synthesizing substantial amount of taurine and extensively used in studies on lipid metabolism, and tried to explain its biochemical basis by investigating several hepatic microsomal membrane-bound enzymes activities related to lipid metabolism.

本实验中，我们将观察牛磺酸对大鼠肝脂质代谢的影响，并观察牛磺酸对大鼠肝脏微粒体脂质代谢酶活力的影响，以探讨牛磺酸影响肝脏脂质代谢的可能机制。

SEA Liposomes has the equal ability to induce TILs proliferation, cytokine secretion and cytotoxicity against HepG-2 cells in vitro as compared with the free SEA.

SEA脂质体对小鼠原位H22肝癌具有明显抑制作用，并能增加肝癌组织TIL浸润和肝脏组织中TNF-α、IFN-γ水平。

The experimental results showed that both electroporation and liposomesenhanced sperms retentin of pSV-lacZ DNA.

实验表明，电脉冲和脂质体可有效地促进外源DNA和精子的结合。

V increased (other than Ara-C) and the inhibitory effect against 〓 improved significantly, especially for ADM. The empty pro-liposomes may undergo three process when hydrated with drug solution: swelling of phospholipid film, fracture of film and resealing to form vesicles.

以空白脂质体前体为载体，对几种临床上常用的抗癌药物的抑瘤活性及毒性进行了研究，研究结果得出：与游离药物相比，5-Fu，阿霉素等药物的抑瘤活性都有不同程度的提高；急性毒性实验表明：除Ara-C外，几种药物的毒性均有所降低，以顺铂最明显。

The results from ultraviolet-spectrometer and TBA methods suggested 〓Cu〓 could cause the liposome peroxidation in the presence of reductants.

使用紫外分光光度计和TBA法发现〓Cu〓在还原剂的作用下会引起脂质体过氧化，这种作用对还原剂和铜离子有依赖性。

Methods Mammalian expression vectors containing human MGMT gene were tranferred into PBMC in vitro via liposome. Realtime PCR and Western blot were used to detect the expression of MGMT at mRNA and protein levels.

利用脂质体法将含入MGMT基因的真核表达载体导入体外培养的外周血单个核细胞，实时PCR及Western blot检测目的基因mRNA及蛋白水平的表达。

MethodsAfter construction of a fulllength human βNGF expression vector, human cholangiocarcinoma cell QBC939 was transfected with pcDNA3.0NGF.

方法构建βNGF的真核表达载体pcDNA3.0NGF，经酶切鉴定后，采用脂质体lipofectamine转染人胆管癌细胞株QBC939。

The transient transfection efficiency was measured by flow cytometry. The stable transfected cells were screened by G418. Results Electrotransfection had the highest transient transfection efficiency (56.8%), with the most masc clones of stably transfection and the least time taken to form big masc clones (20 days). Cationic polymer mediated transient transfection had the lowest efficiency (1.7%), with the least masc clones and the longest time taken to form the big masc clones (30 days). Cationic liposome transfection had a transient transfection efficiency of 39.9% and took 25 days to form the big masc clones.

结果 电转染有最高的瞬时转染效率(56.8%)，稳定转染得到的阳性克隆最多，且从细胞筛选到扩增至得到阳性大克隆所用的时间也最少（约20d）；用阳离子聚合物瞬时转染率低(1.7%)，稳定筛选同样可以得到阳性大克隆，但是数量很少且从细胞筛选到扩增至阳性大克隆所用的时间也最多（约30d）；阳离子脂质体转染效果居中，瞬时转染率为39.9%，从细胞筛选到扩增至得到阳性大克隆所用的时间约25d。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。