脂质体

Result: The result of electron micrography and the size distribution showed that the liposomes were similar to spherical small unilamellar vesicles.The mean diameter was(258.2±51.2) nm and the electric charge of surface is 19.0 mV.

结果：脂质体呈典型单室，近球型，大小相近，分散均匀，平均粒径为(258.2±51.2)nm，表面电荷19.0 mV。

Objective Fluorescent-labeled antisense oligomer that is hybridized to 18-bp sequences next to the start coden of human proliferating cell nucleus antigen gene was used to assess the intracellular distribution and stability of the oligonucleotides in the presence or absence of liposome.

目的 观察硫代修饰型及天然型PCNA反义寡核苷酸在脂质体介导下或直接转染人膀胱癌细胞BIU-87后在细胞内的分布及其稳定性。

While the weight ratio of nanoparticles with DNA was 3:1, the encapsulating efficiency of DNA was about 95% or so.

与脂质体相比，对组织细胞的毒性小。具备了理想基因载体应有的特点。

The matrine liposomes with high entrapping efficiency can be prepared by ammonium sulphate gradien method.

采用硫酸铵梯度法可制得包封率较高的苦参碱脂质体。

Objective To evaluate effect of hemagglutinating virus of Japan -liposome mediated tissue factor pathway inhibitor gene transfer on neointimal hyperplasia of endothelium denuded carotid artery of rat.

目的 探讨日本凝血病毒-脂质体介导的组织因子途径抑制剂基因转移对大鼠颈总动脉内皮剥脱术后新生内膜增生的抑制作用。

So in order to get recombinant NT-3, the rat NT-3cDNA was reconstructed in a mammalian expression vector and transfected into incasing CHO cells by liposome-mediated method. The clones of the cells transferred with rNT-3cDNA were selected by G418. The Chinese hamster ovary cell line stably producing recombinant rat NT-3 was established.

因此，为了获得基因重组NT-3，研究其生物效应，本文成功构建了重组大鼠NT-3基因真核生物细胞稳定表达载体，并利用脂质体介导，转染进CHO细胞；经G418筛选，建立了中国仓鼠卵巢细胞系，稳定表达重组大鼠NT-3。

Abstract］ Objective:To improve Lipofectin mediated transfection efficiency.

目的：提高脂质体介导的细胞转染效率。

Therefore, it is possible that liposome drug delivery system raised the DXR's inhibition effect of metastasizing of carcinoma.

因此，脂质体形成了DXR的体内贮库，延缓了DXR在中央室的消除以及从中央室向周边室的分布，延长了DXR在血液循环系统中的运行时间。

The research were carried out under the different conditions of the sun light, heating water bath, pH value and metal compand to study the stability of the curcumin and its lipsomes.

分别在不同光照、加热、pH及不同的金属离子条件下考察了姜黄素及其脂质体的稳定性以及对其影响程度。

At last,the optimized formula composition and technique of preparation were obtained by orthogonal design,and long-circulating praziquantel liposome was prepared.

在建立吡喹酮含量测定方法的基础上，通过葡聚糖凝胶柱色谱法、显微镜法、激光散射法等定性及定量方法考察脂质体的包封率、微粒外观、粒径及其分布等指标。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。