脂质体

The goat fetal fibroblast cells were transfected by linear GBCt-PAm with electroporation or lipidosome, and after the identification of PCR, we got 7 lines of progenitive cells that examined positive.The two cell line (M9, M10) had been used for the somatic nuclear transfer for the first time.

将pGBCt-PAm去除原核序列后回收线性片段，采用电击和脂质体转染两种方式转染山羊胎儿成纤维细胞，经G418筛选培养基筛选及PCR检测，最终得到了7株能有效扩增的PCR检测阳性的细胞克隆。

Studies of molecular genetics have demonstrated that retinoblastoma susceptibility gene is closely related to the development of retinoblastoma and the theory of Rb gene mutation or inactivation has been putatively proven to be the molecular mechanism of RB formation.

本研究以我国培养建立的一株RB细胞系〓及裸鼠为实验材料和对象，在对〓细胞抑癌基因（Rb基因、p53基因）表达状况进行检测和鉴定的基础上，通过建立裸鼠玻璃体腔RB常位异种移植模型，构建Rb基因的逆转录病毒表达载体，以脂质体介导的Rb基因转移方式，进行RB体内基因治疗的实验研究，观察Rb基因在体内对RB的治疗作用及影响因素，并探讨其作用机制。

To study the assembly and release of adriamycin in vitro using magnetic thermosensitive liposomes treated with magnetic field and pyrogenation.

目的：探讨磁场和加热作用下磁性热敏阿霉素脂质体在体外的聚集及释药情况。

The recombination vector was digested by Pac I enzyme and transfected into 293A cell by Lipofectamine method to obtain recomb...

该载体经PacI酶切，脂质体法转染人胚肾293A细胞，获得重组腺病毒载体pAD-E7.pAD-E7转染人HaCaT细胞，激光共聚焦显微镜分析E7蛋白表达情况。

To monitor transfection efficiency, 50 ng of the pRL-null plasmidencoding Renilla luciferase was included in all samples.

HEK293细胞接种到12孔板和0.5微克的神星启动建设用脂质体转染 2000（Invitrogen公司）根据制造商的说明。

However, the activity decreased for copper salicylate encapsulated in positively and negatively charged liposomes.

但正负电性脂质体包裹的水杨酸铜的SOD样活性降低。

The late phase expression cassette suited for adenovirus vectors was also constructed. The HBV S gene, HBV preS2+S gene and the late phase expression cassette +HBV S genes were respectively cloned into the Ad5 vector downstream of the E3 promoter. Semiconfluent monolayers of 293 cells were cotransfected with the above constructed plasmids and Ad5 DNA EcoRI A fragment. The progeny adenoviruses named rAd5S, rAd5MS, rAd5S2S were harvested.

通过共转染优化实验，建立了脂质体转染腺病毒的有效方法，并构建了适合腺病毒载体的晚期表达盒，进一步将乙型肝炎表面抗原基因、HBV preS2+S、腺病毒晚期表达盒+HBV S基因插入E3区，与EcoRI消化的Ad5 DNA A片段共转染细胞，并获得重组腺病毒，相应命名为：rAd5S、rAd5MS、rAd5S2S。

Identification of cripto-1 as a novel serologic marker for breast and colon cancer［J］.

脂质体介导的cripto反义寡核苷酸抑制结肠癌细胞端粒酶活性［J］。

Objective: To optimize the formulation and preparation process of sinomenine liposomes.

中文摘要：目的：优化青藤碱脂质体的膜材处方，并进行制备工艺研究。

The results show that the nano-magnetoliposomes prepared contain As2O3. They are spheric or elliptic particles with clear magneticcore.

结果所得纳米As2O3磁性脂质体粒径为(190±80)nm，球形或卵球形，有一个明显磁性核心，As2O3的包封率为53.0%。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。