脂质体

Methods Amplify the cDNA sequence encoding truncated HCV gene, with a part of carboxyl-terminus deleted, by PCR. Synthesize the mimic epitope at E2 region of HCV and seven T or Th cell epitope genes of NS3-NS5 respectively. Bind HCV core gene with a part of carboxyl-terminus deleted to the synthetic epitope gene by PCR, then clone into eukaryotic expression vector pcDNA3.1 and transiently transfect COS7 cells.

用PCR方法扩增核心区羧基端部分缺失的基因片段；分别合成HCV E2区模拟表位和NS3~NS5 7个T或Th细胞表位基因；PCR方法将羧基端部分缺失的HCV核心区基因与合成的表位基因串联，克隆入真核表达载体pcDNA3.1，并通过脂质体瞬时转染COS7细胞。

Abstract］ Objective: To investigate the effect of the mycobacteriophage D29 liposome-coating liquid in the mice infected by multi-drug resistant tubercle bacillus, as a new method in treating multi-drug resistant tuberculosis.

［摘要］目的：研究D29分枝杆菌噬菌体脂质体包被液对耐多药结核杆菌感染小鼠的治疗效果，探寻治疗耐多药结核病的新方法。

Mycobacteriophage D29 liposome-coating liquid has a beneficial effect in mice infected by multi-drug resistant tubercle bacillus, which may be a new method in treating multi-drug resistant tuberculosis.

噬菌体脂质体包被液对耐药结核菌感染小鼠具有良好的治疗效果，有可能成为治疗耐多药结核病的新方法。

OBJECTIVE:To optimize the formula and preparation technique of UFT (tegafur/uracil)liposomes.

目的：优化优福定脂质体的处方和制备工艺。

When SA-bFGF-Math1 transferred to the epithelial cells(UEC-4) of utricle,these cells showed the potential to differentiate hair-cell-like cells.

通过脂质体将bFGF-Math1转染大鼠椭圆囊斑的上皮细胞(UEC-4)，可使其分化为新毛细胞。

Methods The whole-length X gene was directly cloned to pcDNA3.1 vector. The recombinant vector (pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin, steady clones (HEPG2-X cells) were obtained. The expression of RhoC protein was analysed with immunohistochemical stain.

用定向克隆的方法构建X基因的真核表达载体pcDNA3.1-X，脂质体转染HEPG2细胞；潮霉素选择培养稳定表达X基因的HEPG2-X细胞；免疫组化鉴定HEPG2-X细胞RhoC蛋白表达。

METHODS: The total RNA was obtained by TRIzol from an antiricin neutralizing monoclonal antibody hybridoma cell named 4C13 and heavychain and lightchain variable fragments were amplified by overlap PCR and accepted by Genbank of NCBI. VH and VL were linked by3 with a direction of VHlinkerVL and cloned to pCDNA3.1 vector expression system.

用TRIzol提取抗ricin中和性单抗杂交瘤细胞4C13总RNA，扩增其轻、重链可变区基因，登陆GenBank；用Linker3将VH和VL连接，用重叠延伸PCR克隆入真核表达载体pCDNA 3.1，用脂质体转染293T细胞，对瞬时表达产物进行生物学活性检测。

The diameter of the arbutin liposomes prepared through the new equipment is 70nm and the envelop ratio is 16.532%.

生产的熊菓苷脂质体的粒径为70nm、包封率为16.532%。

Methods: 1. Leukemia cell lines HL-60、Jurkat、Molt-4 were grown in RPMI medium 1640 supplement with 10% fetal calf serum and 100U/ml benzylpenicillin and 100 U/ml streptomycin sulfate in a humidified atmosphere containing 5% of CO2 at 37oC.

用脂质体将测序正确的重组子转染至白血病细胞株HL-60、Jurkat、Molt-4中，用RT-PCR、Western Blot方法检测iASPP的表达以及用流式细胞仪检测细胞凋亡的变化状况。

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。