脂质体

Objective To investigate the effect of transdermal penetration of arbutin flexible liposome and several promoting reagents.

目的 利用离体皮肤渗透技术研究了熊果苷柔性脂质体的经皮渗透和几种渗透促进剂对其影响。

Methods:The conservative region of mTOR gene was inserted into pLXIN reversedly, then the vector was packaged in PT67 cells by transfection with lipofectamine and transfected to VSMC. The efficiency of antisense inhibition was verified,and the changes of p70S6k and 4E-BP1 were also determined at the same time. The proliferation activity was determined by flowcytometry and MTT.

采用脂质体介导转染包装细胞后构建mTOR的反义重组逆转录病毒载体(pLXIN-AmTOR)感染VSMC，检测mTOR及其下游底物包括真核细胞启动子4E结合蛋白1(4E-BP1)和核糖体蛋白S6激酶(p70S6k)的表达变化，以及VSMC细胞增殖周期和增殖活性的改变。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

The results showed that the C of Econazole nitrate from the prepared liposomal gel in epidermis and dermis 3 days after application were significantly higher than that from the ointment on sale.

研究了硝酸益康唑脂质体凝胶剂在体皮肤用药3天后的表皮和真皮中药物消除过程，求出消除过程中的AUC值，并与市售软膏作了比较。

Ingredients: Liquorices extract, soybean protein, compound vitamin liposome, HA, etc.

主要成份：甘草提取液、大豆蛋白、复合维他命脂质体、透明质酸等。

Ingredients: Liquorices extract, mulberry extract, compound vitamin liposome, HA, etc.

主要成份：甘草提取液、桑树提取液、复合维他命脂质体、透明质酸等。

Results: it was efficient to transfect chicken embryonic fibroblast cells by the method of lipofectamine mediation. the expression levels of protein were significantly different.

结果 脂质体介导的方法能有效转染鸡胚胎成纤维细胞，实验中egfp蛋白相对表达量随质粒量的增加而明显升高（p.01）。

The plasmid DNA remained intact and could be stored at 4℃ for at least one month in liposomes, it was also resistent to DNase I digestion.

重组质粒DNA在脂质体的制备过程中保持了稳定性，并能抵抗DNA酶的水解破坏作用。

AIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS 7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEM T easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N 末端带上含24 bp的flag标签，克隆到pGEM T easy载体并测序，再亚克隆至真核表达载体pcDNA3 1，酶切鉴定正确后采用脂质体法瞬时转染COS 7细胞，Western blot检测flag cbl在细胞中的表达。

METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cAIM: To construct the eukaryotic expressing vector of human cbl and test its expression in African green monkey kidney cell line COS7. METHODS: Human cbl gene tagged with flag was amplified from pEFHAcbl plasmid by PCR. The product was cloned into pGEMT easy, sequenced and then subcloned into eukaryotic expressing vector pcDNA3.1.

以含有人全长cbl cDNA的质粒pEFHAcbl 为模板，采用PCR方法扩增cbl基因，并在其N末端带上含24 bp的flag标签，克隆到pGEMT easy载体并测序，再亚克隆至真核表达载体pcDNA31，酶切鉴定正确后采用脂质体法瞬时转染COS7细胞，Western blot检测flagcbl在细胞中的表达。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。