脂质体

The constructed pcDNA3-IL-15 plasmid was identified by Bgl Ⅱ digestion,PCR amplification and sequenceanalysis respectively,to ensure that the hIL-15 cDNA was properly inserted into thevector and the sequence was correct.Then the pcDNA3-IL-15 plasmid was instantlyexpressed in COS-7 cells by means of lipofectamine transfection,and followed by astable expression in Chinese hamster oval cells.8 positive cell colonies wereobtained after the G418 selection,designated CHOA~H cells.

经过BglⅡ酶解分析，PCR扩增鉴定和DNA测序分析，证明IL-15 cDNA已正确插入克隆载体且序列正确后，采用脂质体法，首先将pcDNA3-IL-15重组质粒转染至非洲绿猴肾细胞（COS-7）做瞬时表达，再转染至中国仓鼠卵巢细胞进行稳定表达。

The methods of purification and reconstitution of Ca〓-Mg〓-ATPase were set up and Ca〓-Mg〓-ATPase from both spontaneously hypertensive rat and control rat were isolated, purified and reconstituted in the study.

建立了质膜钙泵的分离纯化方法和磷脂重建方法并用这些方法成功地分离纯化了正常大鼠和自发性高血压大鼠红细胞膜钙泵，而且将其重建于人工脂质体上。

OBJECTIVE: To investigate liposome-mediated cytosine deaminase gene transfecting rabbit BMSCs and its gene expression.

目的：探讨脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其基因的表达。

OBJECTIVE: To investigate transfection and expression of liposome-mediated cytosine deaminase gene in rabbit BMSCs.

目的：观察脂质体介导胞嘧啶脱氨酶基因转染兔骨髓间充质干细胞及其表达。

By the electropositive Liposome 2000, in the 6-plate for cell culture, pEGFP-P80 above is transfected into the PK-15 cells from the kidney of pigs and expressed.

利用阳性脂质体L2000 介导，在六孔细胞培养板中，将已经构建好的pEGFP-P80重组表达质粒转染到PK-15 细胞中进行表达。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

An MFGM-derived phospholipid fraction was used to produce liposomes via a high-pressure homogenizer.

一个MFGM源性磷脂小部分被用来制造脂质体经高压均质。

The first -order rate constants for the transmembrane transport of the complxes into human erythrocytes were detemined and the mechanism was studied on the basis of the interactions of these complexes with cellular membranes and liposome by ESR,fluorescence technique, mercapto measurement and gelchromatography.

测定了它们跨人红细胞膜的一级反应动力学常数。用膜蛋白巯基滴定、荧光标记、自旋标记ESR谱等方法研究了它们与膜蛋白、膜脂的相互作用；用脂质体模拟了这些配合物的细胞摄入。

Addition of DSPE-PEG-RGDS and Stearyl RGDS in liposomal prescription gives birth to two targetedly thrombolytic liposomes. The former has long circulating function, the latter has no long circularing action but carries two times directing molecules as that carried by the former.

将DSPE-PEG-RGDS和Stearyl RGDS分别掺入脂膜中制备得到了两种靶向溶栓脂质体，前者同时具有长循环作用；后者不具长循环功能，但导向分子的携载量为后者的两倍。

The coupling condition of different lipid ratio, glutaraldehyde concentration and types of liposomes was probed.

探索了不同比例的脂、不同戊二醛浓度和不同类型脂质体的偶联情况。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。