脂质

To monitor transfection efficiency, 50 ng of the pRL-null plasmidencoding Renilla luciferase was included in all samples.

HEK293细胞接种到12孔板和0.5微克的神星启动建设用脂质体转染 2000（Invitrogen公司）根据制造商的说明。

The replicons were detected and characterized by RT-FUR. IFA. Renilla Luciferase assay system and real time RT-FUR. respectively. Results It was shown that SLB2 mutant did out significantly affect the translation of the input RNA.

将突变体（包括相关的阳性和阴性对照复制子）分别线性化并体外转录成RNA后，取等量各转录体RNA，以脂质体法分别转染BHK-21细胞。

Methods:The conservative region of mTOR gene was inserted into pLXIN reversedly, then the vector was packaged in PT67 cells by transfection with lipofectamine and transfected to VSMC. The efficiency of antisense inhibition was verified,and the changes of p70S6k and 4E-BP1 were also determined at the same time. The proliferation activity was determined by flowcytometry and MTT.

采用脂质体介导转染包装细胞后构建mTOR的反义重组逆转录病毒载体(pLXIN-AmTOR)感染VSMC，检测mTOR及其下游底物包括真核细胞启动子4E结合蛋白1(4E-BP1)和核糖体蛋白S6激酶(p70S6k)的表达变化，以及VSMC细胞增殖周期和增殖活性的改变。

However, the activity decreased for copper salicylate encapsulated in positively and negatively charged liposomes.

但正负电性脂质体包裹的水杨酸铜的SOD样活性降低。

Methods: The model was constructed by ethanol administration orally in mice. Salisburia adiantifolia preparation was administered as antioxidant to protect the gastric mucosa.

大鼠用乙醇灌胃造模，给予抗氧化剂银杏制剂预防胃粘膜损伤，测定胃粘膜和血中的脂质过氧化物含量。

By regulating lipids metabolism, chrysalis oil can prevent and cure the formation of nonalcoholic fatty obviously and it was better than the scabish oil.

蚕蛹油可通过调整脂质代谢，具有十分显著的预防和治疗非酒精性脂肪肝形成的效果，优于月见草油。

Objective: To investigate the effect of scutellaria bacalensis stem-leaf total flavonoid on lipid peroxidation induced by myocardial ischemia reperfusion in rats.

目的：探讨黄芩茎叶总黄酮预处理对缺血再灌注心肌脂质过氧化损伤的保护作用。

The late phase expression cassette suited for adenovirus vectors was also constructed. The HBV S gene, HBV preS2+S gene and the late phase expression cassette +HBV S genes were respectively cloned into the Ad5 vector downstream of the E3 promoter. Semiconfluent monolayers of 293 cells were cotransfected with the above constructed plasmids and Ad5 DNA EcoRI A fragment. The progeny adenoviruses named rAd5S, rAd5MS, rAd5S2S were harvested.

通过共转染优化实验，建立了脂质体转染腺病毒的有效方法，并构建了适合腺病毒载体的晚期表达盒，进一步将乙型肝炎表面抗原基因、HBV preS2+S、腺病毒晚期表达盒+HBV S基因插入E3区，与EcoRI消化的Ad5 DNA A片段共转染细胞，并获得重组腺病毒，相应命名为：rAd5S、rAd5MS、rAd5S2S。

Identification of cripto-1 as a novel serologic marker for breast and colon cancer［J］.

脂质体介导的cripto反义寡核苷酸抑制结肠癌细胞端粒酶活性［J］。

Objective To evaluate the reliability of lipid-bound sialic acid and tumor specific growth factor in the diagnosis of lung cancer .

目的用脂质结合唾液酸与恶性肿瘤特异性生长因子对肺癌诊断的对比性研究。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。