脂质

To adopt antiphase evaporation transonic method and high speed agitation method to prepare Semustine Magnetic Liposome, to carry out the exosyndrome with TEM, image analysis system and energy spectrometer, to test the envelopment rate of semustine in the liposome.

采用反相蒸发超声法加高速搅拌制备纳米司莫司汀磁性脂质体，用透射电镜、图像分析系统和能谱仪对其进行表征，HPLC法检验脂质体中司莫司汀的包封率。

The Fe3O4 magnetic nanoparticle is a good carrier to prepare medical magnetic liposome. The adoption of antiphase evaporation transonic method and high speed agitation method can prepare nanometer Fe3O4 magnetic liposome.

Fe3O4纳米粒子是制备医用磁性脂质体的良好载体，采用反相蒸发超声法加高速搅拌可制备纳米级Fe3O4磁性脂质体。

Objective To investigate the influence of lipid metabolism by splenectomy and whether semi-splenectomy and splenic autotransplantation are beneficial for LM.

目的 探讨脾切除对脂质代谢的影响，以及保留部分脾和自体脾移植是否对脂质代谢产生有益的作用。

The formulation was optimized on the basis of orthogonal design and its entrapment efficiency was performed by the protamine sedimentation method .

方法采用薄膜蒸发一高压均质法制备槲皮素脂质体，通过正交设计优化处方工艺，以鱼精蛋白沉淀法分离脂质体与游离药物，测定药物的量。

The both diets could also reduce the serum levels of triglyceride and free fatty acids, but the CLA diet caused an increase in the serum levels of total lipid and cholesterol. In contrast, the OFO diet caused a reduction in the serum levels of total lipid and the cholesterol.

血脂质方面，CLA饮食与炸油饮食均会使血清三酸甘油酯与游离脂肪酸降低，但CLA饮食会增加血清总脂质与胆固醇含量，相对餵食炸油饮食其血清总脂质与胆固醇仍然降低。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

The apolipoproteins on the surface of the lipoproteins help to solubilize the lipids and target the lipoproteins to the correct tissues .

在脂蛋白表面的载脂蛋白有助于溶解脂质，并把脂蛋白导向适当的靶组织。

The water and oil are removed from soybean oil degummed residues, and then separated into two parts by using 95% ethanol extraction.

为促进和拓宽磷脂在医药学领域的应用，进行了锆镁基质脂质体色谱固定相制各及药物与脂质膜相互作用研究。

The laboratory research and chinical trials to prove.courage lcecontains active material that it can quickly penetrate through body's cell.so to directly provides for the cell,fill the skin intercellular space the crevice which is senile withered because of the cell stays benind supply the active elements necessary for the body cells metabolism,to activate the function of endothelium cells of blood vessels,promote the blood vessels balance, compose and secrete various blood vessels active elements,contains abundantsulfuric cartilage element,sulfuric amido etc.,all of fatty acid in human body.and it can accelerete the re-generation of blood cells,add the quantity of the hemoglobin and red blood corpuscle so to improve the blood parameter,and fundamentally improve the body cells metabolism and nutrition supply for all the organs,as a result,you will thoroughly refresh yourself and bloom vitality of youth.

实验室研究和临床试验证明，LCE所含活性物质能够迅速穿透人体细胞，直接为细胞供养，填补肌肤细胞间因细胞衰老干瘪而留下的空隙全面补充人体细胞新陈代谢所必须的活性物质，激活血管内皮细胞功能，促进血管平衡，合成和分泌多种血管活性物质：富含的硫酸软骨素、硫酸氨基葡萄糖等能改善和调节人体脂代谢，有效清除体内血液中多余的脂质和脂蛋白清除在血管壁内膜沉积的脂类物质，保持人体脂肪酸均衡：能够加快血液细胞更新，提高血液中的血红蛋白和红血球数量，改善血液参量，从根本上改善人体细胞的新陈代谢，改善人体各组织器官的营养供给，使人由内而外脱胎换骨，重新焕发青春活力。

The laboratory research and chinical trials to prove.courage lcecontains active material that it can quickly penetrate through body's cell.so to directly provides for the cell,fill the skin intercellular space the crevice which is senile withered because of the cell stays benind supply the active elements necessary for the body cells metabolism,to activate the function of endothelium cells of blood vessels,promote the blood vessels balance, compose and secrete various blood vessels active elements,contains abundantsulfuric cartilage element,sulfuric amido etc.,all of fatty acid in human body.and it can accelerete the re-generation of blood cells,add the quantity of the hemoglobin and red blood corpuscle so to improve the blood parameter,and fundamentally improve the body cells metabolism and nutrition supply for all the organs,as a result,you will thoroughly refresh yourself and bloom vitality of youth.

实验室研究和临床试验证明，LCE所含活性物质能够迅速穿透人体细胞，直接为细胞供养，填补肌肤细胞间因细胞衰老干瘪而留下的空隙全面补充人体细胞新陈代谢所必须的活性物质，激活血管内皮细胞功能，促进血管平衡，合成和分泌多种血管活性物质：富含的硫酸软骨素、硫酸氨基葡萄糖等能改善和调节人体脂代谢，有效清除体内血液中多余的脂质和脂蛋白清除在血管壁内膜沉积的脂类物质，保持人体脂肪酸均衡：能够加快血液细胞更新，提高血液中的血红蛋白和红血球数量，改善血液参量，从根本上改善人体细胞的新陈代谢，改善人体各组织***的营养供给，使人由内而外脱胎换骨，重新焕发青春活力。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。