脂质

Electron microscope observations showed that the liposome suspension was heterogeneous and the particle size ranged from 20 nm to 700 nm with a average diameter of 163 nm. The vesicles showed unilamellar and multilamellar structure which consisted of several concentric bilayers.

电镜观察表明，该脂质体多呈球形的单层或多层结构，脂质体粒径分布均匀，粒径范围20-700 nm，平均粒径163 nm，主要粒径分布位于50-200 nm(84.80%)。

Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体；用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠，用血凝抑制实验和酶联免疫吸附试验对免后2～13周的小鼠血清的抗体水平进行检测，HI检测其特异性抗体，ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

Methods Femoral arteries isolated from the rabbits which had been fed with a high cholesterol diet and locally perfused with MM-LDL within the artery beforehand, were used as the models. Antisense MCP-1cDNA was transferred into the arterial wall by injecting recombinant LNCX-anti-MCP-1/liposomal complex in the femoral sheath and the periarterial tissue.

以实验性高脂血及轻度氧化LDL局部保留灌注的家兔股动脉为模型，采用DOTAP阳离子脂质体包裹LNCX-anti-MCP-1质粒，将反义MCP-1 cDNA定位导入家兔股动脉鞘及周围组织，以原位杂交、 Northern杂交及狭缝杂交法观察反义MCP-1基因的表达，应用扫描电镜观察单核细胞在动脉内膜粘附的情况。

Methods M. penetrans was cultured and its LAMPs were extracted. Mouse macrophages were stimulated with M. penetrans LAMPs to analyze the production of nitric oxide. Cell prolification was detected in M. penetrans LAMPs-stimulated mouse macrophages by MTT assay.

用SP-4培养基培养穿透支原体并提取其脂质相关膜蛋白，用提取的脂质相关膜蛋白刺激小鼠巨噬细胞，以ELISA法检测NO的产生，并用MTT法检测其对小鼠巨噬细胞增殖的影响。

rubia cordifolia l can promote the mice capacity of antioxidation and reduce the content of mda, have obvious improve great mouse's sports ability.

补充茜草提取物可显著提高小鼠心肌和肝脏组织抗氧化酶的活性，减轻大强度耐力训练对大鼠心肌和肝脏组织造成的脂质过氧化损伤，具有明显的抵抗脂质过氧化的功能，对小鼠运动能力的提高有良好的作用。

Methods BALB/c nude mice were subcutaneously transplanted with SCG-7901 tumor cells, then the mice were randomly divided into antisense oligonucleotide group, sense oligonucleotide group and the control group. The HIF-1α ASOND complexed with cationic liposome, HIF-1α SOND complexed with cationic liposome and liposome were injected intra-tumorally in the above groups, respectively.

用人胃癌细胞株SCG-7901建立胃癌裸鼠皮下移植瘤模型，将荷瘤裸鼠随机分为反义寡核苷酸组、正义寡核苷酸组和对照组，瘤体内分别注射阳离子脂质体包裹的HIF-1αASOND、HIF-1αSOND和空白阳离子脂质体。

Viral vector mainly contain adenovirus, retrovirus,adeno-associated virus and so on, but it has potential danger of safety; it isrepeled by immune system when it is injected to organism for a greatimmunogenicity. An injection with adenovirus vector with highconcentration, it leads to serious inflammatory reaction of liver. Viralvector such as liposome and polycation are commonly used lately. But,liposome and polycation have low specificness and targetness of genetransfer tissue, have lower transfection efficiency and short period ofgene expression, for they can be phagocytized by endothelial system.

许多的载体，病毒载体和非病毒载体以前已广泛应用，病毒载体主要包括腺病毒，逆转录病毒，腺相关病毒等；但病毒载体在安全性方面存在潜在的危险；免疫原性比较强，注射到机体后很快会被机体的免疫系统排斥，当静脉注射高浓度的腺病毒载体会使肝脏发生严重的炎症反应；非病毒载体目前常用的有脂质体及多聚阳离子聚合物；但脂质体和阳离子聚合物介导基因转移缺乏组织的特异性和靶向性，转染效率较低且易被网状内皮系统吞噬，基因表达时间短；因此研制新型的非病毒载体已成为研究的热点，纳米颗粒具有小尺寸效应，表面效应，随着颗粒直径变小，比表面积将会显著增大，故具有很高的化学活性，因而纳米成为了最有应用前景的非病毒载体。

Aim To prepare triamcinolone-acetonide-acetate-loaded solid lipid nanoparticles carbomer gel with tripalmitin glyceride, and investigate their characteristics and transdermal drug delivery.

目的以醋酸曲安奈德为模型药物，以三棕榈酸甘油酯为脂质材料制备醋酸曲安奈德固体脂质纳米粒卡波姆凝胶，考察其特性以及药物经皮渗透性能。

Results: in control group, there were no atherosclerotic changes on endarterium; in model group, there were obviously atherosclerotic changes on endarterium, in "National pits1573" 520、450、380 Alcohol group and 450 control Alcohol group, there were unconspicuously atherosclerotic changes on endarterium.

结果：空白对照组主动脉内膜未见粥样硬化改变；模型组主动脉内膜粥样硬化斑块形成，部分斑块病灶范围较大；45°对照酒组仅见少量散在脂质条纹；&国窖1573&52°、45°组，仅见轻度主动脉粥样硬化改变；38°组，主动脉壁见散在少量小范围脂质条纹。

To improve the stability of small unilamellar vesicles in vitro, the permeability of SUV from soybean phosphatidylcholine coated by collagen and chitosan was studied respectively by using 5(6)-Carboxyflourescein5(6-CF as a fluorescence probe.

为了提高小单层脂质体（SmalUnilamelarVesicles，SUV）的体外稳定性，以5（6）－羧基荧光素（5（6）－CF）为荧光探针，研究了脂质体被胶原蛋白和壳聚糖这两种天然生物大分子包覆后的通透性。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。