脂腺

The recombination vector was digested by Pac I enzyme and transfected into 293A cell by Lipofectamine method to obtain recomb...

该载体经PacI酶切，脂质体法转染人胚肾293A细胞，获得重组腺病毒载体pAD-E7.pAD-E7转染人HaCaT细胞，激光共聚焦显微镜分析E7蛋白表达情况。

AIM and METHODS:We adapted the none circulating method perfused rat heart and high performance liquid chromatography technique and compared with the control group, the ischemia reperfusion group and the Acanthopanox Senticosus group, studied the effect of Cordyceps Sinensis succi on the content of adenine nucleotides and MDA during ischemia reperfusion.

目的和方法：观察冬虫夏草对缺血再灌注过程心肌保护作用。采用Langendorff非循环式离体心脏灌流方法，与正常对照组、缺血再灌组、刺五加注射液用药组相对比，研究了冬虫夏草醇提取物对大鼠心肌缺血-再灌注过程脂质过氧化产物和腺嘌呤核苷酸含量的影响。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活，ICAM-1表达升高，与被激活的PMN表面表达的LFA-1相结合，&PMN-EC&相互作用加剧，一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍；另一方面粘附的PMN过度吞噬或激活，当白细胞吞噬的颗粒不能被封闭隔离，连同细胞内的酶被释放出来，其中的PMN-elastase能够降解细胞基质中各种成分，水解多种蛋白，加重胰腺的毛细血管内皮细胞和腺泡的损伤；释放的溶酶体酶使组织蛋白水解，产生的不饱和脂肪酸引发脂质过氧化方应，破坏细胞膜的结构和功能；释放的炎性细胞因子，激发其他的免疫细胞的功能，导致进一步的组织损伤和坏死，加重SAP的病理损伤，最终导致休克、脓毒血症及多器官功能障碍等严重后果。

After 8 weeks of experiment,the mice were killed,the coefficients of thymus and spleen,the amount of bone marrow DNA,the contents of peroxid lipids in the liver were detected.

试验期间定期进行体重测定。8周后处死小鼠，测定胸腺脾脏系数、骨髓DNA含量、肝脏组织过氧化脂质含量。

AIM: To investigate the protective effect of verapamil on lipopolysaccharide-induced pancreatic acini damage and its mechanism.

目的：研究维拉帕米对脂多糖导致的大鼠胰腺腺泡细胞损伤的拮抗作用及其机制。

Because the follicle opening is wider, the sebum (sebaceous glands produce a substance called sebum, which is responsible for keeping the skin and hair moisturized) and the sloughed off dead skin cells react chemically with the air, resulting in the oxidation of melanin , which in turn causes the dark coloration of the blackhead.

因为毛囊的状态开放得比较大，皮脂（脂肪分泌腺产生的一种物质叫皮肤，它是用来起到为皮肤和毛发的保湿作用的）和脱落的皮肤死皮细胞在空气中发生化学反应，导致黑色素的氧化，从而使黑头变黑。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

MethodsSixty Wistar rats were divided into 2 groups randomly: normol group(n=12), and model group(n=48).The model rats were fed with high fat-sugar diet for 6 weeks, then fed with adenine(100 mg·kg-1·d-1) and ethambutol(250 mg·kg-1·d-1) for 2 weeks to induce the IR and hyperuricemia model.

方法选用 60只成年Wistar雄性大鼠，随机分成正常对照组（12只）和模型组（48只），模型组给予高脂高糖饲料餵养6周，6周后再用腺嘌呤〔100 mg/kg·d）〕加乙胺丁醇〔250 mg/〕灌胃2周，造成胰岛素抵抗并高尿酸血症的大鼠模型。

MethodsSixty Wistar rats were divided into 2 groups randomly: normol group(n=12), and model group(n=48).The model rats were fed with high fat-sugar diet for 6 weeks, then fed with adenine(100 mg.kg-1.d-1) and ethambutol(250 mg.kg-1.d-1) for 2 weeks to induce the IR and hyperuricemia model.

方法选用 60只成年Wistar雄性大鼠，随机分成正常对照组（12只）和模型组（48只），模型组给予高脂高糖饲料喂养6周，6周后再用腺嘌呤〔100 mg/kg·d）〕加乙胺丁醇〔250 mg/〕灌胃2周，造成胰岛素抵抗并高尿酸血症的大鼠模型。

Efficiency: Dredge gland pipes, strengthen the formulation of fat, keep gland tissue complete and firm, powerfully replenish and reconstruct fat ground substance, stimulate cells breath and metabolism, centralize fat, and make breast full and pointed.

功效：以通畅腺管，强化脂肪之增生，能使腺体组织健全且结实，更能深层补充及重建脂房基质，并能刺激细胞呼吸与新陈代谢，充分使脂肪集中，赋予胸部组织丰满与坚挺外观。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。