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脂肪酶

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The enzymes derived from pigs is called pepsin and is haram. Another enzymes derived from pigs or small cattle is lipase.

来源于猪的酶被称作胃蛋白酶,是haram的;另外来源于猪或小牛的酶被称作脂肪酶

Pancreatin provides the three major families of enzymes: proteolytic, amylase, and lipase.

胰酶提供了三大主要的酶素:蛋白酶、淀粉酶和脂肪酶

Then, the specific activities of protease and amylase were relatively lower than pancreatin separating with acetone, but the specific activity of lipase was higher. The ratio of activities in filtrate were very low, showing the precipitation was effective.

其中蛋白酶和淀粉酶的活力较低,与丙酮胰酶(3159U/g和58.7kU/g)相比有较大的差距,但是脂肪酶活力较高,高于丙酮胰酶的50.10kU/g。

When substrate molar ratio(ethyl caprylate/fish oil)15∶1 and 10% immobilized Lipozyme RM IM with added 30% water are mixed for 11 h at 45℃ SC-CO_2 and SC-CO_2 pressure 12.5 MPa,the interesterification activity of lipase is most effective and the highest incorporation of caprylic acid with triacylglycerol is obtained.

当反应温度为45℃,底物摩尔比为15∶1,酶用量为底物总量的10%,添加水量为酶量的30%,反应时间为11h,超临界CO2压力为12 5MPa时,脂肪酶LipozymeRMIM的催化效果最好,辛酸结合率在反应产物甘三酯中达最大值。

Transesterification of geranol and vinyl acetate in ionic liquids were investigated to synthesize geranyl acetate using immobilized Candida antarctica lipase B as biocatalyst. The reuse of ionic liquids and lipase were also studied in this reaction.

以固定化的南极假丝酵母脂肪酶为催化用酶催化香叶醇和乙酸乙烯酯在离子液体中的酯交换反应,合成了香料乙酸香叶酯,同时通过该反应了解了酶和离子液体的重复利用性。

The results indicate that Ca(superscript 2+) and GA3, single or combined application treatments, promote cucumber seed germination treated with P. harmala alkaloid, increase germination rate, germination vigor and index; as well as α-amylase, protease, lipase, isocitratelyase, nicotinamide adenine dinucleotide kinase and Ca(superscript 2+)-ATPase activities; and GA and CaM content in cucumber seeds; but decrease abscisic acid content. The synergistic effects of combined Ca(superscript 2+) and GA3 on increasing GA and decreasing ABA are oberseved compared with Ca(superscript 2+) treatment.

结果表明,Ca(上标 2+)和GA3单一及复合处理可促进骆驼蓬生物碱处理的黄瓜种子的萌发,使黄瓜萌发率、发芽指数和活力指数提高,萌发种子的α-淀粉酶、蛋白酶、脂肪酶和异柠檬酸裂解酶活性及赤霉素和钙调素含量增加,烟酰胺腺嘌呤二核苷酸激酶和Ca(上标 2+)-ATP酶活性增强,脱落酸含量降低;且Ca(上标 2+)和GA3复合处理对GA含量的提高和ABA含量的降低与Ca(上标 2+)单一处理比较呈显著的加成作用。

In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题,采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

The substrate specificity test showed that the lipolytic enzyme from strain DF-B6 was an esterase, and not a lipase.

底物特异性实验确定DF-B6菌株分泌的脂裂解酶为酯酶,而非脂肪酶

Esterases and lipases have been widely applied in various industrial process and thermophilic esterases have a great potential application for their thermo-stability.

中文摘要酯酶和脂肪酶已经被广泛的应用于多种工业中,嗜高温酯酶因优良的热稳定性有很大的应用潜力。

The effects of lipase dosage, pH, hydrolysis time and temperature on the foaming properties of egg albumen powder were researched. Results indicated that the optimal hydrolysis conditions were lipase dosage 5000 U/g, pH value 8.0, hydrolysis time 6 h and temperature 42℃.

试验考察了酶添加量、pH值、水解时间、水解温度对蛋清粉起泡性能的影响,确定了脂肪酶水解脂类的较适条件为酶添加量5000 U/g,pH值8.0,水解时间6 h,水解温度42℃。

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