脂肪酶

Through screening of lipases, lipase Novozym435 was found to show the highest activity and enantioselectivity for the ammonolysis of CHBE. The effect of different solvents on the ammonolysis was investigated.

经过对脂肪酶及反应溶剂的筛选，确定最佳脂肪酶及溶剂分别为Novozym435和二氧六环；并在该反应体系中考察了温度、底物浓度、酶浓度与摇床转速对反应的影响。

High active enzyme was selected from several lipases which can catalysed the ammonolysis of unnatural sbstance trimethylsilylmethyl acetate.

从不同来源的脂肪酶中筛选出能催化非天然底物———乙酸三甲基硅甲酯的氨解反应且具有较高活性的脂肪酶，并首次探讨了氨源、有机介质、反应初始水活度和温度对该酶促反应的影

Lipase is a surface-activated enzyme and, therefore, interface plays an important role in the course of catalysis.

作为本论文工作的第三部分，研究了脂肪酶微乳液的凝胶固定化条件及固定化脂肪酶的一些酶学性质。

The prosequence had no obvious effects on the characteristics of RCL, which was different from Rhizopus oryzae lipase.

与米根霉脂肪酶不同，前导序列对华根霉脂肪酶的主要酶学特性没有显著影响。

LPL was expressed in many tissues in tree shrew, highly in cardiac muscle and adipose tissue. In despite of no expression in skeletal muscle, there was less-medium expression in liver, distinctly different from human LPL which was expressed in the contrary way in these two tissues.

树鼩脂蛋白脂肪酶的组织表达谱比较广泛，在心肌和脂肪组织中大量表达，但在骨骼肌中未检测到，却在肝脏中有中低水平表达，与人脂蛋白脂肪酶在这两种组织中的表达相反。

We make chloroacetylaion to N-terminal of Asp, and then convert this compound to GD dipeptide by aminolysis. Followed the lipase -catalyzed peptide synthesis of RGD in aqueous water-miscible organic cosolvent is studied. We set up a new efficient reaction method to synthesize RGD tripeptide. The problem that peptide containing polar amino acid can not be synthesized readily in organic solvent was solved. We offer a new experimental proof for PPL-catalyzed peptide synthesis and make a more general analysis for the effect of some factors in theory.

采用氯乙酰基保护天冬氨酸，然后进行氨解生成GD二肽，利用猪胰脂肪酶催化合成RGD三肽，着重解决了亲水氨基酸/肽片段底物的溶解性与合成产率低的问题，建立了一种新的有效合成RGD三肽的反应模型，并探索解决了极性较强的氨基酸的酶促肽合成的问题，对脂肪酶催化合成肽的影响因素从理论上作了较全面的分析，为肽片段的缩合反应提供了一种新思路。

Lipase mediates esterification between caproic acid (0.6mol/L) and ethanol (0.6mol/L) in n-heptane at 40℃. The decrease in caproic acid is measured by titration and 1 unit of lipase synthetic activity is defined as 1 μmol caproic acid consumed per min.

在40℃下，脂肪酶催化己酸(0.6mol/L)与乙醇(0.6mol/L)在正庚烷中进行酯合成反应，通过滴定法测定剩余的己酸，1个脂肪酶有机相合成酶活单位定义为：在测定条件下每分钟消耗1μmol己酸的酶量。

It was increased ethyl hexanoate synthesis substrate concentration mediated by Rhizopus chinensis 2113 whole-cell lipase after improved synthetic activity and optimized reaction condition. The reaction condition were: caproic acid concentration 2.4mol/L, substrate molar concentration ratio 1:1.1, initial water content ≤0.2%, lipase water activity 0.3~0.66, enzyme amount 16%, reaction temperature 30℃, agitation speed 150 r/min.

通过合成酶活的提高及反应条件的优化，提高了华根霉2113全细胞脂肪酶催化己酸乙酯合成的反应底物浓度，其反应条件为：己酸浓度为2.4mol/L；酸醇摩尔浓度比1：1.1；反应体系含水量≤0.2%；脂肪酶水活度0.3～0.66；加酶量16%；反应温度30℃；150r/min振荡反应。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

The relative activity of a lipase from Candida valida Z6 was studied in hydrolysis of simple esters,fatty acid glycerides and lipidse.

通过与一种不同来源的脂肪酶作比较，研究了粗状假丝酵母诱变株Z6产脂肪酶水解不同底物的相对酶活，包括对低级酯、脂肪酸甘油酯和天然油脂的水解。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。