脂肪酶

METHODS: Adipose was obtained from regions surrounding epididymis, retroperitoneal space and nape after anesthesis by pentobarbital, and adipose-derived mesenchymal stem cells could be harvested through type Ⅰ collagenase digestion. ADSCs were incubated in vitro and purified by differential adhesion.

戊巴比妥耳缘静脉麻醉，依次无菌取出附睾附近脂肪垫、腹膜后脂肪、颈背部脂肪，I型胶原酶酶解法分离出脂肪源间充质干细胞（adipose-derived mesenchymal stem cells，ADSCs），进行体外传代后差速贴壁培养纯化。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

The pharmacodynamics and therapeutic function of Ganzhixiao decocting prescription to fatty liver as the following:1. Adjust lipometabolism:reducing TC and TG;Decreasing fatty degeneration of liver cell;2. promoting the convalescence of hepatocellular function;3. Anti-lipid peroxide:increasing the the activation of peroxidase, decreasing the content of productsof lipid peroxide.

肝脂消颗粒剂治疗脂肪肝的效应及药效学机制为： 1、调整脂质代谢：使肝酶活性增强，降低血清甘油三脂和胆固醇； 2、减轻肝细胞脂肪变牲； 3、促进肝细胞功能恢复，有效治疗脂肪性肝炎； 4、抗脂质过氧化：提高抗氧化酶活性、降低脂质过氧化产物含量。

The results of asymmetric reduction of latent chiral ketones by carbonyl reductase showed that the enzyme had a high specificity for pentanone in aliphatic ketones and for the acetophenone derivates that contained a withdrawing group at α-position.

利用羰基还原酶不对称催化还原潜手性酮类化合物发现，对于芳香酮类化合物，酶对α位为强电负性基团的底物专一性较强；对于脂肪酮类化合物，酶对五碳脂肪酮的专性较高。

Which can be used as the body of vitamin B1 coenzyme of many enzymes in the normal human body has an important role in glucose metabolism, it can inhibit the activity of cholinesterase, and choline esterase is an important neurotransmitter, and thus nerve conduction impact; vitamin B2 in the organization by participating in a variety of yellow enzymes constitute a coenzyme, its role in bio-oxidation process in the delivery of hydrogen role in regulating the body's metabolism process; vitamin B6 can also be a variety of enzymes as coenzyme participate in all kinds of amino acid metabolism, promote the absorption of amino acids and protein synthesis, cell growth is necessary for the material, which also promote fat metabolism, involved in neurotransmitter synthesis, hemoglobin synthesis and the impact on the normal reproductive functions such as human body an important metabolic process, Volkswagen Medicine Net: What liver protection effect of vitamin B family?

可作为维生素乙许多酶的辅酶，在正常人体组织中有一个用糖代谢的重要作用，能抑制胆碱酯酶活性，和胆碱脂酶是一个重要的神经递质，神经传导，从而影响；由黄色参与各种酶在组织中维生素B2构成辅酶，其作用在生物氧化氢的作用，调节人体的代谢过程中产程；维生素B6也可以是作为辅酶参与氨基酸代谢的各种酶的品种，促进氨基酸的吸收和蛋白质合成，细胞生长是必要的材料，这也促进脂肪代谢的神经递质合成，血红蛋白合成和参与，如影响人体生殖功能的正常新陈代谢过程中一个重要的，（大众医药网：什么样的维生素B家庭肝脏的保护作用？

The uptake of 〓I-OxLDL in adipocytes was determined by gamma ray counter and normalized to protein concentration of each sample, was expressed as nanograms of 〓I-OxLDL uptake per milligram of cell protein. Reverse transcription polymerase chain reaction was used to evaluate PPARγ and CD36 mRNA expressions.

采用酶联免疫吸附法检测血浆IL-6以及脂肪细胞分泌IL-6的水平；采用gamma射线计数仪来检测脂肪细胞对〓I-OxLDL的特异性摄取情况，采用改良Lowry法进行细胞蛋白浓度测定，细胞对〓I-OxLDL的特异性摄取量以ng／mg细胞蛋白表示；采用逆转录聚合酶链式反应来检测PPAR γ和CD36 mRNA在脂肪细胞的表达情况。

In the second chapter, the hydrolysis of olive oil catalyzed by Candida cylindracea lipase in Aerosol OT /isooctane reverse micellar solution was studied.

第二章从工业应用的角度对酵母脂肪酶在AOT/异辛烷逆胶束系统中催化橄榄油水解的全过程进行了考察和优化。

The esterification reaction kinetics of hexanoic acid with butanol, catalyzed by Novozym 435 lipase, was studied in water-in-oil microemulsions formed by alkyl polyglucoside C10G1.54/n-butanollcyclohexane/water buffer solution.

研究了Novozym435脂肪酶在烷基聚葡糖苷形成的W/O微乳液体系中，催化正丁醇与正己酸合成酯的反应动力学。

The esterification reaction and itsinfluencing factors of hexanoic acid with n-butanol, catalyzed by lipaseNovozym100T, were studied in water-in-oil microemulsions formed by alkylpolyglucoside C_(12)G_(1.50)/n-butanol/cyclohexane/water system.

研究发现，十二烷基聚葡糖苷C_(12G_(1.50)／正丁醇／环己烷／H_2O体系形成的油包水微乳液中，脂肪酶Novozym100T催化正己酸和正丁醇的酯合成反应，反应初始速率V_0随正己酸的初始浓度C_~0增大而增大；随正丁醇的初始浓度C_~0的增大而减小，后V_0进入一&坪区&。

The effect of the flight on the activities of energy metabolism related enzymes in the flight muscles of the Oriental armyworm moth, Mythimna separata females was studied. These enzymes measured were glyceraldehydes-phosphate dehydrogenase, glycerol-3-phosphate dehydrogenase, lactate dehydrogenase, and 3-hydroxyacyl-CoA dehydrogenase. In the first 5 min of the tethered-flight of 3 day-old moth, activities of all the enzymes related to carbohydrate and lipid metabulistu increased rapidly, enzymes related to lipid metabolism were completely activated, and the activities of HOAD were significantly strengthened. But in flight duration from 5th to 60th min, the activities of all energy metabolism related enzymes decreased, suggesting that the flight was going to be stabilized.

对3日龄粘虫雌蛾吊飞过程中4种相关酶3-羟酰辅酶A脱氢酶、3-磷酸甘油醛脱氢酶、3-磷酸甘油脱氢酶和乳酸脱氢酶的研究结果表明，在室内条件下，粘虫在吊飞过程中其能量代谢有以下特点：在吊飞的初始5min，所有与糖代谢和脂肪代谢相关的酶活性都快速升高，这段时期脂肪代谢的酶活性也完全被活化，HOAD活性明显增强；但在随后的5~60min持续吊飞期间与能量代谢有关的酶活性都有所下降，表明此时飞行活性趋于平稳。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。