脂肪酶

Alkaline lipase Greasex 50L is one of the leather enzyme products of Novo Nordisk AS. After extensive control trials on pigskins, evaluating effects by degree of fat removal and qualities of result leather, we got1 Fat removal rate can be increased by 10%and leather qualities such as napping and softness can be improved by using Greasex 50L together with liming enzyme NUE 0.6 MPX2 Some surfactant together with Greasex 50L is good to fat removal, and the recommended ratio is 0.05%～0.1%∶13 The fat content in the skins is lower after treated with Greasex 50L,which also benefits the successive processes, such as bating, pickling and tanning. As a result, the leather qualities, such as fineness of grain, softness, napping and evenness of dyeing can be improved.

Greasex 50L是诺和诺德公司生产的碱性脂肪酶已在皮革工业中广泛使用，为了更好地应用这种酶制剂，我们对Greasex 50L在猪皮方面的应用条件进行了研究采用对比的方法，用脂肪去除率和皮革的质量作为评价标准，研究结果表明1适量的浸灰酶 NUE 0.6 MPX可使Greasex 50L的脱脂率增大，同时可改善皮革的起绒状况和革身柔软性2一定量的表面活性剂与Greasex 50L配合使用，有利于脂肪的去除，用量为平平加的0.05%～0.1%3 经Greasex 50L脱脂的裸皮在鞣制后油脂含量也较低，有利于后续工序进行，从而使成革粒面平细、革身柔软，臀部起绒得到改善，染色均匀性良好

In order to extract insoluble dietary fiber from coconut residue,monofactorial tests were carried out based on component analysis of coconut residue to determine the appropriate levels of factors influencing proteolysis and adipolysis,orthogonal test was accomplished to optimize technological conditions of one-step zymohydrolysis and the product's function was determined too.

为提取椰子渣不溶性膳食纤维(insoluble dietary fiber，IDF)，在测定椰子渣化学组成后经蛋白酶和脂肪酶分步酶解的单因素试验初步确定影响酶解各因素的适宜水平，在此基础上采用正交试验优化蛋白酶和脂肪酶一步酶解制备IDF的工艺条件，并测定产品的性能。

Compared with the previous method, the improved route can provide directly a spheroid form of immobilized lipase particles of which the size can be controlled by adding non-ionic surfactants during the preparation process so as to increase the recovery rate of enzyme as well as decrease the external diffusional restriction which can diminish the enzyme activity. The improved method can also alter the hydrophobicity and microstructure of the immobilized enzyme matrix by changing the molar ratios of different alkoxyl silanes so as to enhance the enzyme activity by a"surface active effect"and eliminating the internal diffusional restriction.

与前人的制备方法相比，改进的方法可直接制得球形的固定化酶颗粒，而且颗粒的大小可以通过在制备过程中加入非离子型表面活性剂加以控制，从而可以提高固定化过程的酶活回收率和减小外扩散效应对酶的表观催化活力的影响；改进的方法还可以通过改变原料硅氧烷的种类及配比来改变最终制得的固定化载体的疏水性质及微观结构，从而通过对脂肪酶的&界面活化&作用和减小内扩散效应提高固定化脂肪酶的表观酯化活力。

In order to make use best of these resources, the lipase-catalyzed acidolysis of lard with caprylic acid to produce functional lipid was investigated.

脂肪酶筛选实验表明，在所选用的五种脂肪酶中，来自T.languginosa的同定化脂肪酶Liopzyme TL IM的催化效果最好。

The optimum conditions of lipase pretreatment and the optimum ratio of lipase and laccase were investigated in this paper.

摘 要：利用脂肪酶具有降解脂肪和蜡的特性，将漆酶与脂肪酶混合处理麦草，以提高蒸煮药液的渗透性，改善蒸煮性能。

The expression of cloned cold-adapted lipase gene lipA in Pichia pastoris GS115 with the pPIC9k as the expressive vector was performed according to the methods described in the manual, version 3.0, of the pPIC9K identified the lip gene cloned here was a functional lipase gene.

以pPIC9K为表达载体，以GS115为表达宿主菌，成功构建重组表达质粒pPIC9K-lipA，经甲醇诱导表达，能够分泌有脂肪酶活性的表达产物，因此证明本研究所克隆的脂肪酶基因lipA确实为脂肪酶开放阅读框。

We use plate to check the lipase activity ,the lipase activity is very low, Compared to the wild type , the mutant could not form the clear halo on the olive oil plate but tributyrin plate, it showed that the 227 th Serine was essential for the lipase activity.

与野生型相比，突变体脂肪酶的酶活很低，不能在橄榄油平板上形成透明圈，但可以在以三丁酸甘油酯为底物的平板上形成透明圈，表明第227位丝氨酸可能对脂肪酶酶酶活具有重要影响。

Fat tissue is hydrolyzed and released fatty acid as well triolein by lipase; on the other hand, high fat diet can't be directly absorbed across the intestinal mucosa.it must be firstly decomposed into one 2-monoglyceride and two free fatty acids by pancreatic lipase ,which is delivered into the lumen of the gut as a constituent of pancreatic juice.

脂肪酶能够使体内的多余脂肪组织分解成三酸甘油酯，另一方面，人体摄入高脂肪食物，并不能直接被小肠粘膜吸收，而是先被小肠内的胰脂肪酶分解成2-单酸甘油酯和两个游离脂肪酸，然后和胰液一起被运输到肠道的内腔被吸收。

In addition, the effluences of the enzymedchicken fat as a participant in Maillard-reaction on the flavor of final products were compared with that of the unenzymed one.

通过研究鸡脂肪酶解风味变化情况，寻找出其酶解程度对风味影响，确认酶解最佳工艺条件；通过美拉德反应比较了酶解与未酶解鸡脂肪对最终产品风味的影响程度。

Novozym 435 was used for the synthesis of vitamin A plamitate. The influences of solvent, the molar ratio of substrates, the reaction temperature and time, and the water concentration were optimized and the best result was obtained by interesterification from 0.200 g vitamin A acetate and 0.468 g palmitic acid, at 30℃, in 10 mL hexane, containing 0% of water, with 10% of lipase(mass ratio, immobilized lipase to vitamin A plamitate). In these conditions, 75% of vitamin A acetate was converted into vitamin A plamitate. The immobilized lipase was reused about 6 batches.

对催化合成维生素A棕榈酸酯反应的脂肪酶和反应介质比较，同时对影响合成维生素A棕榈酸酯反应的因素（温度、初始水含量、底物摩尔比、反应时间和酶量等）进行了探讨，优化了反应条件：在10 mL不含水分的正己烷中，0.200 g 维生素A醋酸酯和0.468 g棕榈酸在酶量为10％（指固定化酶与维生素A醋酸酯的质量比）的固定化脂肪酶催化下，在 30℃、190 r/min下反应6 h，转化率可以达到75%，固定化酶可连续使用6次以上。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。