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The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明:成功构建了pET22b-lysB表达载体,并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白;His-LysB具有分解脂肪的能力,属于脂肪酶;生物化学特性分析表明:丁酸对硝基苯为水解底物,His-Lys热稳定性不佳,30℃以下比较稳定,随着温度的升高,稳定性逐渐降低;该蛋白具有较高的pH值适应性,pH 5.0~9.5范围内稳定性较高;在23℃和pH 7.5时酶活力最高,其比酶活为1.3 U/mg;金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

Although lipomatosis are painless and pathologically benign, discomfort and disfigurement encourage these patients to seek the removal of such lesions.

虽然多发性脂肪瘤是无痛、良性的,但是不舒服感及畸形的外观,让病患想将这些脂肪瘤移除。

Objective: To raise the awareness and diagnosis level of pelvic lipomatosis associated with proliferation cystitis; to illustate the imaging characteristics and clinical manifestations; and to understand the relevance of pelvic lipomatosis arid proliferation cystitis.

目的:提高对盆腔脂肪增多症合并腺性膀胱炎的认识与诊断水平,了解盆腔脂肪增多症、腺性膀胱炎的影像特点与临床表现以及两者发病的相关性。

Multiple symmetric lipomatosis (Madelung's disease) is a rare disease characterized by non-encapsulated adipose deposits in the neck, the superior part of the trunk and, very rarely, in the limbs. It is common in middle-aged Caucasian Mediterranean males but very rare in Chinese people.

多发对称性脂肪沉著病(Madelung's disease)是一种罕见的疾病,主要的特徵为脂肪组织广泛的沉基於颈部及上胸部,一般好发於地中海地区中年男性且有酗酒习惯者,发生於中国人种更属相当罕见。

By analyzing MRI, MRU and clinical manifestations altogher, pelvic lipomatosis associated with proliferative cystitis can be correctly diagnosed using MRI images. Because proliferative cystitis can easily evolve into bladder cancer, patients with pelvic lipomatosis associated with proliferative cystitis should pay great attention and to perform timely follow-up checkups.

根据MRI、MRU与临床特点的综合分析,MRI能够正确诊断盆腔脂肪增多症合并腺性膀胱炎,由于腺性膀胱炎易演变为膀胱癌,因此,对于盆腔脂肪增多症合并腺性膀胱炎的患者应高度重视,密切随诊复查。

The discrimination between ordinary lipoma and atypical lipomatous tumor/well-differentiated liposarcoma is a common diagnostic challenge in diagnostic soft tissue pathology.

普通型脂肪瘤和非典型/高分化脂肪肉瘤的鉴别诊断是软组织病理学诊断工作中的一个难点。

Microscopically, retroperitoneal well differentiated liposarcomas appear identical to the well circumscribed atypical lipomatous tumors of neck, shoulder, upper back and other regions in extremities.

显微镜下,腹膜后分化良好型脂肪肉瘤在形态上与发生在颈、肩、上背和四肢其他部位的境界清楚的非典型脂肪瘤相似。

Lipomatous tumors in internal auditory canal and cerebellopontine angle are very rare; low-grade papillary adenocarcinoma of endolymphatic sac origin is rarely reported and has low malignant potential.

脂肪瘤样迷离瘤是一种发生在内耳听神经管或桥小脑角非常罕见的由脂肪、纤维、平滑肌、横纹肌以及神经成分组成的良性肿瘤。内耳内淋巴囊来源的低级别乳头状腺癌是发生在内耳内淋巴囊的特别罕见的低级别肿瘤。

Intra-oral lipoma is a well-known entity, but lipomatous tumors including salivary gland tissue containing clustered or peripherally located ducts and acinar cells are uncommon.

脂肪瘤是一种已广为人知的良性肿瘤,但在脂肪的肿瘤中含有唾液腺组织(包括唾掖腺管道与胪泡细胞)并不常见。

The conclusion indicated that pyruvate supplement could accelerate the speed of body energy metabolism and slower weight and body fat gain during exercises. Due to the influences of pyruvate supplement on the levels of enemies and hormones to adjust body lipometabolism during exercises, body blood lipid and glucose levels decreased, and the process of fat metabolism came under.

丙酮酸补充可在一定程度上减慢运动机体体重和体脂的增长速度,加快运动机体能量代谢速度;并通过影响运动机体脂肪代谢相关调控酶和激素水平,使其血脂和血糖水平下降,从而影响其脂肪代谢过程。

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然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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