能结合的

In general, the design work with traditional measures is done by hand, which cannot meet the needs of the technology and manufacture. So the author has developed a CAD hydraulic design software fit for lower specific speed axial flow runner, which is easily operated by users.The main research work of this paper is as follows:1 Analyzing the main reason of axial flow power station cannot work well under littler volume of flow in some season and putting forward an improving scheme, pointing out the characters of design under abnormal factors.2 Combined with the development of the industry in our country, the abnormal hydraulic design method of propeller turbine is discussed based on present literature, data and experiment results.3 Guided by the design method of software engineering, a suit of hydraulic design software of Lower Specific Speed Axial Flow Runner is programmed by the object-oriented programming language Visual Basic and a straightforward, concise and standard Windows human-computer dialogue interface is designed for the system, which makes the operation of the system very convenient.4 ActiveX Automation technology is adopted as the interface with AutoCAD and the hydraulic drawings are edited, modified by AutoCAD until hydraulic model drawings according with the demand of engineering practice are output.5 To resolve practice problem, the author has designed a lower specific speed axial flow runner for Duowa Power Station through an example.

论文的主要内容有：1 深入分析很多轴流定桨式水电站枯水季节小流量下不能发电的主要原因，提出减容改造方案，并指出超常规条件下的改造设计特点。2 结合国情及行业发展需要，参考现有资料、文献和前人的研究成果，进行低比转速轴流式转轮叶片水力设计方法的研究，对轴流式转轮叶片的奇点分布法水力设计进行了全面的探索，结合现代计算机技术，找到了一种针对特殊条件的轴流式转轮叶片水力设计方法。3 仔细比较多种AutoCAD二次开发工具后，结合本课题的特点，选用Visual Basic语言，综合应用软件工程的设计思想，进行低比转速轴流式转轮叶片水力设计软件的编制，为系统设计了直观、简洁和标准的Windows人机对话界面，便于用户操作；编程时在考虑到设计条件特殊性的同时，尽量做到程序的通用性，使之可用于解决同一类问题；另外，大部分程序还可移植到普通轴流式转轮叶片水力设计中，具有良好的适应性。4 利用ActiveX Automation技术与AutoCAD接口，对AutoCAD进行二次开发，在AutoCAD下实现水力图的自动生成和编辑，直至输出符合实际工程要求的木模图。5 通过算例，为多哇水电站设计了一个低比转速轴流定桨式转轮，解决其在枯水期小流量下不能发电的问题。

Then, the system is linearized by variational approach, the local null controllability is proved by applying a generalized implicit function theorem and combining the good property of the solution mapping.

首先通过对系统线性化，构造泛函，利用对偶方程，给出控制函数具体形式的办法得到系统的逼近能控性；然后采用变分方法对系统线性化，再结合解映射好的性质，应用推广的隐函数定理，证明系统的局部零能控性；最后利用局部零能控性和逼近能控性结合给出系统零能控的结论。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

The regulation of gene expression is to have relations to the assembly of the DNA binding proteins which bind to DNA to initiate the transcription. Some of these proteins have the ability to promote bending or looping of DNA. The FIS protein regulates gene expression in Escherichia coli by this property of bending DNA. There are many researches to discover the mechanism of FIS protein binds to DNA. However the experiment data only show the supposititious results and figure out the model of FIS-DNA complex structure. This research uses molecular dynamics simulation to compute the detail of the interaction between FIS and DNA. We observed FIS prominently affects the DNA bending by the type of local bending. Analyzing the DNA structure properties, roll and slide, also demonstrates the local bending type and region. The hydrogen bonds between FIS and DNA are analyzed to compare with the experiment data. Finally we compute the binding free energy between FIS and DNA to estimate the result of simulation and compare with the value of λ-repressor-DNA complex.

基因的表现与结合於DNA促使DNA转录作用起始的DNA结合蛋白组装有关，一些这类的蛋白质具有促使DNA扭曲或将DNA形成环状的能力，FIS蛋白质即是藉由扭曲DNA的特性调控大肠杆菌基因的表现，目前已有很多的研究发现FIS与DNA结合的机转，然而这些实验的数据仅能显示推测性的结果，进而利用这些结果推论出FIS与DNA聚合体结构的模型，本研究利用分子动态模拟的方式计算FIS与DNA交互作用的细节，我们观察到FIS主要影响DNA的扭曲方式为DNA区域扭曲的类型，并且分析DNA结构特性roll与slide也指出扭曲方式为区域扭曲与其扭曲的区域，我们也分析FIS与DNA之间的氢键，并且与实验的资料作比较，最后我们计算FIS与DNA之间的结合自由能评估模拟的结果，并且计算出的值和λ-repressor与DNA聚合体结构的实验值作比较。

This suggested there might have cis-acting element in 3'-UTR. In order to search the possible trans-acting factor, we found RNA-protein band in H〓 cells and no band in 95D cells using 3'-UTR probe by RNA EMSA assay. At the same time, this band could be competed with the unlabled probe. There was also no band obsearved using 5'-UTR probe in both cells.

为了寻找可能与3'-UTR结合的蛋白质反式作用因子，用RNA凝胶阻滞(electrophoresis mobility shift assay，EMSA)的方法，我们在H〓细胞的胞浆蛋白质提取物与3'-UTR探针的结合反应中，发现了一条RNA-蛋白质的结合带，而在95D细胞没有结合带出现；而且这种结合带能被未标记探针竞争性抑制；5'-UTR的结合反应也没有观测到结合带的形成。

After the identification of SARs in Attacus ricini rRNA gene, its rRNA gene and NTS were also found to have the ability binding with the nuclear scaffold of Saccharomyces cerevisiae. The 1kb NTS fragment which can bind clearly with nuclear scaffold of Attacus ricini was the chief fragment binding with yeast nuclear scaffold. This indicated the homology between this two kinds of SARs and provided an evidence for the evolutionary conservation of SARs.

在鉴定蓖麻蚕rRNA基因中具有骨架结合区域之后，利用体外结合的方法发现，蓖麻蚕rRNA基因及其非转录间隔区片段也具有与酵母核骨架结合的能力，尤其是能与蓖麻蚕核骨架结合的非转录间隔区1kb片段，也能明显地结合于酵母核骨架，这一结果表明二者的SAR顺序具有一定的同源性，为骨架结合区域具有进化保守性提供了又一证据。

Two major pathways exist in the reactions of Cu〓, Ag〓, Au〓 with ethanethiol clusters, one producing 〓 and the other forming〓.

实验观察到Cu〓、Ag〓、Au〓能结合的乙硫醇数目依次为1～10、1～4、1～2，说明它们与乙硫醇团簇的反应活性逐渐减小；它们与乙硫醇的反应都生成了〓，n的大小按Cu〓、Ag〓、Au〓的顺序逐渐增大，由此推测Cu〓与乙硫醇团簇反应时生成〓为主要反应通道，Au〓与乙硫醇团簇反应时生成〓为主要通道，这两种通道并存于Ag〓与乙硫醇团簇的反应中。

A competitive inhibitor is usually structurally similar to the substrate, e.g. malonate is a competitive inhibitor of the enzyme succinate dehydrogenase.

与底物结构相似的物质能竞争性的与酶结合，从而使酶不能与底物结合而丧失其催化活性。

Skill-mental dominated event-group has characteristics what are polarizing frighten goal and fluctuating variation course. 6 Coaches and athletes must insist on the principles that is comminuting of judging accurately and intervening in good time, perspective with getting practical to combine together, timeliness and synchronous combine together, controllability and principle that substantial results combine together.

影响竞赛过程中运动员竞技能力变异的众多因素可聚合为以下四个相对独立又相互联系的子系统，即：运动员的竞技状态与相对竞技实力、参赛认知水平与比赛经验、可逆转环境和中立环境。4运动员竞技能力的参赛变异具有变异成因的多元性和多变性、变异内容体能的先导性、变异过程的可控性与非可控性、变异结果的两极性和层次性和参赛行为的心态易变性等五个方面的一般训练学特征。5体能主导类项群具有变异结果直观性、变异过程连续性和对手制约两重性等特征；技能主导类项群具有单项贡献制约性、个体变异传递性和裁判评定诱导性等特征；技战能主导类项群具有群体整合共生性、核心技战术制导性、攻防转换制约性和局盘赛点突增性等特征；技心能主导类项群具有目标恐惧极化性和变异过程波动性等特征。6教练员、运动员必须坚持准确判断和适时干预相结合、前瞻性与务实性相结合、适时性与同步性相结合、可控性与实效性相结合的原则。

The method has such advantages as easy operation, stable process and no pollution. The product, when used for fatting leather, can improve the quality, softness and toughness of leather, and can tightly bond with leather fibers. The bonded fatting agent does not transfer in leather, and has such advantages as rapid permeation and uniform distribution, thus can make leather soft and moist.

本发明操作简便，工艺稳定，无环境污染；用本发明制备的产品对皮革进行加脂，不仅能增加成革的丰满性、柔软性、柔韧性，而且能与皮革纤维结合牢固，结合的加脂剂在皮革中不易发生迁移，还具有渗透快，分布均匀等特性，可使皮革柔软、滋润、经久而不枯板。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。