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The results of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L,there existed some stable hydrophobic regions,which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid,in the partially folded intermediate of Bacillus amyloliquefaciensα-amylase;with the denaturation concentration increasing,the stable hydrophobic regions disappered.the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed that using acrylamide as quenchers,with the protein denaturation extent increasing,the number of Trp that can be quenched increased untill all the Trp residues were quenched;Using potassium iodide as quenchers,with the maximum number(8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciensα-amylase molecule could be quenched by potassium iodide;with the denaturation concentration increasing,the number of Trp that can be quenched decreased to 5.the results of their protein electrophoreses and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciensα-amylase formed during the whole unfolding/refolding procedure of Bacillus amyloliquefaciensα-amylase induced by guanidine hydrochloride or urea.

ANS外源荧光探针结果表明：盐酸胍诱导的芽孢杆菌α-淀粉酶分子去折叠过程中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；而随着芽孢杆菌α-淀粉酶分子在盐酸胍溶液中变性程度的加深，这一疏水区域逐步被瓦解。丙烯酰胺和碘化钾猝灭结果表明：在盐酸胍溶液中，随着芽孢杆菌α-淀粉酶分子变性程度的进一步加深，其分子内能够被丙烯酰胺接近的色氨酸残基逐渐增多，直至全部被猝灭。但位于芽孢杆菌α-淀粉酶分子表面的能够被碘化钾猝灭的色氨酸残基，在中间态芽孢杆菌α-淀粉酶分子中数目达到最大的8个，而随着其分子变性程度的进一步加深，反而减少至5个。

SKILLS, QUALIFICATIONS AND EXPERIENCE NECESSARYThese should include:- A good knowledge of practical engineering skills- Ability to communicate with the customer quality and engineering department with high level of confidence.- Ability to communicate in English both written and Verbal, communication skill in French would be advantageous.- Educated to Degree level or equivalent in a relevant engineering discipline- Experience in process design and development, prototype parts production and project planning- Be familiar with major customer project planning techniques- The ability to understand measurement data and take necessary action to ensure product features achieve required capability (1.67 Cpk)- Have extensive knowledge of pressings, welding technologies- An appreciation of Kaizen, 6 Sigma, and common improvement techniques.- Possess excellent organisation, communication and information systems skills including use of MS Project.- Leadership qualities, ability to succeed and display a 'can do' attitude - Understand and compliance in working with company quality standards such as TS 16949

拥有丰富的实际工作经验和工程技术知识；2、能够与客户质量和开发部门进行良好沟通和建立高度信任；3、英语听、说、读、写流利，法语良好者优先；4、工程技术或相关专业本科以上学历；5、具有工艺设计、开发、样件生产和制定项目计划的经验；6、熟悉主要客户的项目计划要求；7、能够理解测量数据的含义和采取必要措施保证产品特性达到过程能力（1.67 Cpk）；8、掌握丰富的冲压和焊接技术知识；9、了解&改善&，6 西格马和现行的持续改进技术。10、具有良好的组织、沟通能力和信息系统技能，包括熟练使用MS Project；11、具有领导素质，能够取得成功和显示&能做到&的态度；12、了解和执行公司的质量标准和体系，如TS16949质量体系。

When cultured on the medium with different auxin , root segments could elongate and branch, and the addition of 1mg/l IBA into the medium was the most suitable for long-term root culture. Calli could be induced from root explants in the presence of 2, 4-D and when transferred onto E medium, they transformed further into embryonic calli which could develop into normal plants through the stages of globular-embryo, heart-shaped embryo, torpedo-shaped embryo, cotyledonary embryo.

培养基中添加生长激素（IAA，IBA，NAA）能够诱导无菌苗根切段的生长和分枝形成；而1mg／l IBA的存在，最适合于根的长期离体培养。2，4-D的存在能够诱导根外植体脱分化形成愈伤组织，愈伤组织转移至E型分化培养基能够转变成胚性愈伤组织，并进一步经球形胚、心形胚、鱼雷形胚、子叶形胚发育成正常植株。

Keeping the Meeting On Target 转入正题We're running short of time.Well, that seems to be all the time we have today.

写作能力可以分为三个层次：(1)能够写作通顺得体的信函，有效的新闻报道和个人记述；(2)能够写作某一专业领域的技术论文或技术文章；(3)能够创作优美的散文和诗歌。

We can produce any different pitch blade according to customer needs, the classic value of the pitch: A, 10 degree, suitable brush motor FP helichoper, let lower speeds rotor greater lift; B, 8 degree, suitable brushless motor FP helichoper, can make the main rotor get a higher speed; C, 6.5 degrees, suitable some DIY FP helichoper, can make the helicopter have better lock tail performance with a small diameter and high-speed tail rotor.

桨距可以根据客户需要进行定制，经典的桨距值为：A、10度，适合有刷马达的FP电直，能够在较低的转速下取得更大的升力；B、8度，适合无刷马达的FP电直，能够使主桨获得更高的转速；C、6.5度，适合玩家DIY的FP电直，配合小直径高转速的尾桨，能够获得更好的锁尾表现。

Approximately three - four dogs can watch the herds including up to 1000 sheep, and even a boy can be a herdsman driving a bicycle and doing everything he wants. The dogs will do everything by themselves.

大约3至4只中亚就能够看守1000只左右的羊，所以甚至连一个小孩都能够当牧人，而且他可以同时做任何他想做的事，中亚能够自己做任何事情。

The results indicated Paecilomyces farinosus was aerobic fungus and rotate speed of rocking bed exceeded 150r/min would benefit the mycelium growth.Initial inoculum could affect the time of reaching maximal mycelial bioass and could not affect the maximal mycelial bioass.inoculum exceeding 5% would benefit t...

结果表明粉拟青霉是一种好气性真菌，摇床转速大于150r/min有利于该菌生长；接种量对菌丝最大产量影响不大，但是能够影响菌丝量达到最大值的时间；接种量大于5%，有利于菌丝产量迅速达到最大值；粉拟青霉菌在pH值为3～10的培养基中都能够生长，生长过程中能够分泌代谢产物主动调节pH值，该菌在pH为5～7的微酸环境中生长最快；液体发酵过程中粉拟青霉生长量与发酵时间的关系符合逻辑斯蒂生长模型。

We need that one guy who can give us that push over the hump or at least cause a diversion and create shots so the others to get back in rhythm.

我们需要一个球员能够帮助球队冲过这个关卡，或者至少能够带来改变，为其他人创造机会，让其他人能够找回手感。

My job at Microsoft is as challenging as ever, but what makes me "tap-dance to work" is when we show people something new, like a computer that can recognize your handwriting or your speech, or one that can store a lifetime's worth of photos, and they say,"I didn't know you could do that with a PC!"

我在微软的工作和以前一样充满了挑战，但是让我能够像跳踢踏舞一样工作的动力便是：当我们向人们展示一些新事物，比如一种能够辨别你的手迹和语调的计算机，或者是一种能够储存你整一生照片的计算机，然后人们会说：&我从没想过你们竟然能用一台家用计算机做出那样美妙的东西来！&

Charlie and I are lucky. We have jobs that we love and are helped every day in a myriad of ways by talented and cheerful associates. No wonder we tap-dance to work. But nothing is more fun for us than getting together with our shareholder-partners at Berkshire's annual meeting. So join us on April 30th at the Qwest for our annual Woodstock for Capitalists.

查理跟我真是幸运，每天能够从事我们喜爱的工作，同时由这么多有干劲的同仁协助，也难怪我们每天都能够边跳舞上班，当然每年能够与股东伙伴们同乐更是一大乐事，所以再次欢迎大家到Qwest中心参与资本主义家的伍斯达克年度音乐会。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。