能发育的

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

猪是多胎动物，从猪卵巢上获得的卵母细胞数量虽然较多，但成熟度比较低；由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内，跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应；如果卵胞质成熟或活化程度不够，加之圆形未成熟精细胞成熟度比较差等原因，很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足，引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等，使ROSI显微受精失败。

When the embryonic original cell divided to form multicellur proembryos, the cell wall of the external cell was thickened to form a clear cell boundary to separate from ambient cells that was a phenomenon of isolation The protoderm began to be differentiated in the period of globular embryoid, and the procambium differentiated clearly in period of torpedo embryoid.

正常的球形胚和某些畸形球形胚继续发育，在心形胚或鱼雷胚阶段又出现出畸形胚，可以分为：①无茎端生长点；②原形成层发育不正常；③外形畸形，如有的是连胚，有的呈指状，有的呈叶片状等等。畸形胚有的败育，有的能继续发育，在子叶胚阶段形成畸形的植株。

This paper has studied the physiological and biochemical changes during the period of forming strong buds of tree peony, and expect to provide academic basis for extending its view value and economic benefit . The main results are as the following:1 Under the field condition, Characteristics of photosynthesis of tree peony are very regular. From before blooming to the prophase of abducting buds that after blooming ,that is April,May and June,the net photosynthetic rate of tree peony is higher than the other period .It indicates that this period is very important for accumulating photosynthetic products of tree peony . So, if we want to get quality flower of tree peony ,it is very important to strengthen the management of tree peony cultivation and to ensure provide the fertilizer and water.2 From the middle ten days of June, with the time elapsing and with the environment conditions worsening, the characteristics of photosynthesis of tree peony decline deeply. But it is different among different tree peony breeds. For example , cv. Wu Long Peng Sheng can also has higher net photosynthetic rate, it indicates that cv. Wu Long Peng Sheng is super than the other two tree peony breeds when adapting the environment conditions and cv. Wu Long Peng Sheng has wide prospect to extend .3 The main factors of effecting the characteristics of photosynthesis are stomatal factor and non-stomatal factor ,that is the ability of photosynthesis of leaf cells. Under the environment of high light intensity in midsummer, if overshadow tree peony properly, then it can slow the declining of chlorophyll content, and increase the net photosynthetic rate and benefit for the accumulating of photosynthetic products.4 The content of soluble sugar in the root of tree peony is the highest among different organs during the period of forming strong buds . According to analyse ,we believe that the soluble sugar will compose starch then, and provide the solid base of material for the growth and development next year. It provide us strong academic basis of adopting the method of cultivating root and protecting root under thefield environment conditions .In other words ,it will help to increase the health level of the whole plant of tree peony and will be beneficial to forming quality tree peony flower if we strengthen the management of root. 5 To tree peony, the endogenesis hormone is important for its growth and development .In the prophase ,that is about the blooming of tree peony and the mid-summer ,IAA and ZR contents are higher and GA content is lower and its changes are very little, and the content of ABA is decreasing . This indicates that IAA and ZR act the important promote function for growth and development of tree peony in this period, and the function of GA is not important. Bur in the period of anniversary senescence of tree peony , the content of IAA and ZR begin to decrease ,while the ABA content has a certain rebound .T

本研究对几个牡丹品种壮芽形成过程中的某些生理生化变化做了研究，以期了解牡丹生长发育过程中生理生化的变化规律，探讨大田栽培条件下提高牡丹花质量的栽培措施和管理技术，为提高牡丹的观赏价值和经济效益提供理论依据，结果如下：1 大田栽培条件下，牡丹光合特性在生长发育过程中表现出极强的规律性，牡丹各品种的光合速率从牡丹开花前，到牡丹花谢后的花芽诱导期前期（6月10日之前），也就是在4、5、6月份，各牡丹品种的净光合速率相对与其他时间都维持在比较高的水平，这说明，在这段时间，是牡丹光合产物积累的重要时期，因此，要保证得到高质量的牡丹花，在这段时间加强对牡丹的栽培管理，保证这个时期的肥水供应十分重要。2 从6月中旬后，随时间的推移，环境条件的恶化，牡丹的光合性能剧烈下降，但是不同牡丹品种间仍有差异，比如，乌龙捧盛仍可维持较高的光合水平，这说明了乌龙捧盛这个牡丹品种对于环境的适应性较之其他牡丹品种高，有着广泛的推广前景。3 影响牡丹光合性能的主要因子是气孔因素和非气孔因素即叶肉细胞的光合能力，在盛夏高温高光强的环境条件下，对牡丹进行适当遮荫，能缓解牡丹叶绿素水平的下降，适当提高Pn，有利于光合产物的积累。4 可溶性糖在牡丹各品种的壮芽形成过程中，根中可溶性糖的含量在牡丹各部位中始终维持最高水平，分析认为这些可溶性糖在根系中进一步合成淀粉等储藏性物质，为来年牡丹的生长提供雄厚的物质基础，这为我们在大田栽培条件下对牡丹采取养根护根的栽培措施提供了有力的理论依据，即在大田栽培条件下，加强对牡丹根系的管理，有助于提高牡丹整株的健壮程度，有益于形成高质量的牡丹花。5内源激素对牡丹的生长发育调节作用是十分明显的，在前期，即开花前后和盛夏，IAA和ZR含量维持在比较高的水平，GA含量低且变化不明显，ABA含量逐渐降低，说明了IAA和ZR对牡丹在这段时间内的生长发育起到重要的促进作用，GA的作用不是很明显，ABA可能在高温高光强下有明显的升高，也说明了ABA的确有一些促进作用，但是到了牡丹周年衰老期，IAA和ZR的含量开始下降，ABA含量却有了一定程度的反弹，说明了IAA和ZR的作用开始降低，ABA起到了促进衰老的作用

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

Fertile stamens 3, in a few flowers only anterior 2 fertile, posterior 1 depauperate or absent, often anterior 2 also depauperate; filaments densely white lanate; staminodes 3; antherodes 3-sect. Capsule ovoid, 1.5--1.7 cm, acute at apex.

能育雄蕊3，在数花内只前面的2枚能育，后面1发育不全或无，通常前面2也发育不全；花丝浓密的白色绵状毛；退化雄蕊3；退化花药 3全裂；蒴果卵球形，1.5-1.7厘米，锐尖的在先端。

Our study displayed that HmgD overexpression mutant died as late third instar larvae and malformed pupae during the larvae-prepupa transition,suggests that overexpression of HmgD has effects rather on the third instar larvae stage than on the first and second instar larvae from the mutant could not pupate.

结果表明，HmgD广泛过量表达突变体幼虫在一龄和二龄期发育正常，而在三龄幼虫化蛹时发育异常，发育停滞在三龄幼虫末期至预蛹的阶段，即HmgD过量表达的幼虫不能正常化蛹。

No tapetal cells were formed or tapetam collapsed at very early stage in some anther chamber during the microsporogenesis; Pollen mother cells in another chamber were not developed and microspore vacuolized; Tapetum cells developed abnormally, giantized and crammed the anther chamber; Some tapetum cells developed normally, but PMCs developed abnomally, which deformed at sporogeneous stage, and then were abhensive each other and finally degenerated. Nearly no PMCs could complete the process of meiosis, and form the tetrad, leading to the failure of normal microspore development.

药室的绒毡层细胞不形成或提前解体；药室内的花粉母细胞不发育或小孢子液泡化；绒毡层细胞发育异常，出现巨型化而挤满整个药室；绒毡层细胞虽发育正常，但花粉母细胞发育异常，在造孢细胞时期即开始出现变形现象，随后发生粘连退化；绝大部分花粉母细胞不能完成正常的减数分裂过程形成四分体，导致小孢子退化。

The situation of abnormal development of male cells is as follows:microspore mother cell can't enter into meiosis because of intense vacuolation,shrink and disintegration of its cytoplasm;although vacuolated microspore mother cell can enter into meiosis,it can't form normal dyad and degenerate in the middle process;dyad and tetrad become vacuolated and can't develop normally;cytoplasm of microspore shrinks around the nucleus at the stage of central nucleus microspore,the shape of microspore is twisted into crescent or irregular shape,at last its cytoplasm and nucleus are disintegrated and crescent vacant microspore presents;nutritive substances can't be accumulated at the stage of vacuolated microspore,cytoplasm is disintegrated,and microspore turns into a big vacant pollen.

雄性细胞异常发育有几种情况：小孢子母细胞强烈液泡化，细胞质收缩解体，不能进入减数分裂；小孢子母细胞液泡化，虽能进入减数分裂，但不能形成正常二分体而中途退化；二分体、四分体细胞液泡化，不能进行正常发育；单核小孢子中央期，细胞质收缩包围核，小孢子形状扭曲呈月牙形或不规则形，最终细胞质和核解体而呈月牙形的空壳小孢子；单核液泡期的小孢子不能积聚营养物质，细胞质解体而成为大的空壳花粉粒。

Rhythm has swinging cradle should be training new child vestibular balance after the first step, then, through the newborn can be turned prone exercises, as a pre-school preparation to climb through the hearing with the eyes, as the objects and the subsequent The combination of the rise, not only promoted the development of infants and neck muscles, but also with the management of audio-visual and other sensory nerve muscle contact.

摇篮有节律摆动应是锻炼新后儿前庭平衡能力的第一步，以后，通过对能转头的新生儿的俯卧练习，作为学爬前的准备，通过听觉与抬眼视物以及后来的抬头的结合，不仅促进了婴儿颈部肌肉的发育，也使视听等感觉与管理肌肉的神经联络。

In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。