能力缺失

Suffering from abulia; showing abnormal inability to act or make decisions.

遭受意志力丧失的；表现出行为或决断的反常的能力缺失。

Methods The clpE-deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The impact of clpE mutant on the virulence of S. pneumoniae was evaluated in a mouse model. In addition, we also studied the effect of clpE mutant on adherence and invasion of host cells. Real time RT-PCR was used to measure the mRNA expression levels of autolysin A, pneumococcal surface adhesion A, pneumolysin, pneumococcal surface protein A and neuraminidase The clpE gene was replaced completely by erm cassette.

用长臂同源多聚酶链式反应方法失活clpE基因，用PCR、测序鉴定缺失菌株，通过动物实验观察clpE基因缺失株毒力改变情况，同时用细胞实验比较clpE基因缺失株和野生菌对宿主细胞的粘附和侵袭能力，最后用实时荧光定量PCR分析自溶素(major autolysin A，lytA)、表面黏附素A(pneumococcal surface adhesion A，psaA)、溶血素(pneumolysin，ply)、肺炎球菌表面蛋白A（pneumococcal surface protein A， pspA和神经氨酸酶（neuraminidase， nanA）的表达。

To better understand the roles of LsrA protein in nodulation, a series of analyses of nodules using scanning electron microscopy, genetic and biochemical approaches have been carried out. Our analyses suggest that the lsrA1 nodules contain bacteroids in the invasion and establishing zone only. The lsrA gene expression is active early in the invasion zone and activated later than bacA genes. The lost of LsrA protein dramatically reduced the expression of nifA and fixK, and completely blocked the expression of the nifH gene for nitrogenase. LsrA protein functions early in the bacteroid development and it is essential for the development nitrogen fixing bacteroids.

前期的工作中，苜蓿中华根瘤菌Rm1021中90个候选LysR基因已经被定向插入突变，并筛选在自生生长时期、共生生长时期的表型，以期寻找更多在自生状态或共生固氮中有功能的LysR转录因子。1 针对前期鉴定出的共生固氮必需的lsrA基因，我们应用了一系列扫描电子显微镜技术、生物化学、分子遗传学等方法，发现lsrA基因主要在根瘤侵染区开始表达，表达时序也在侵染阶段左右，但晚于bacA基因表达；LsrA蛋白缺失后根瘤固氮区中缺乏具有固氮能力的类菌体，nifA和fixK基因的转录水平降低，nifH基因的转录被完全阻断，因此LsrA蛋白为根瘤发育所必需，是新的根瘤发育信号传导途径成员。2 通过表型筛选我们鉴定了苜蓿中华根瘤菌的oxyR基因，并研究了它的调节特性。oxyR突变后，苜蓿中华根瘤菌对过氧化氢敏感性提高，适应性降低。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Goodbye Alice in Wonderland Jewel It's four in the afternoon I'm on a flight leaving L.A. Trying to think about my life My youth scattered along the highway Hotel rooms and headlines I've made a living with a song Just a guitar as my companion Wanting desperately to belong Fame is filled with spoiled children They grow fat on fantasy I guess that's why I'm leaving I crave reality So goodbye Alice in Wonderland Goodbye yellow brick road There is a difference between dreaming and pretending I did not find paradise It was only a reflection of my lonely mind searching For what was missing in my life I'm embarassed to say the rest is a rock and roll cliche I hit the bottom when I reached the top I never knew it was you who was breaking my heart I thought you had to love me You did not Yes a heart can hallucinate If it's completely starved for love Can even make monsters seem like Angels from above You forged my love like a weapon And turned it against me like a knife You broke my last heartstring But you opened up my eyes So goodbye Alice in Wonderland Goodbye yellow brick road There is a difference between dreaming and pretending That was not love in your eyes It was only a reflection of my lonely mind wanting what was missing in my life And growing up is not the absence of dreaming It's being able to understand the difference between the ones you can hold And the ones that you've been sold Dreaming is a good thing cause it brings new things to life Pretending is an ending that perpetuates a lie Forgetting what you are Seeing for what you've been told Well, truth is stranger than fiction And this is my chance to get it right Life is much better without all of your pretty lies So Goodbye Alice in Wonderland You can keep your yellow brick road there is a difference between dreaming and pretending There's not tears in my eyes It was only a reflection of my lonely mind searching It was only a reflection of my lonely mind finding I find what's missing in my life

再见，梦游仙境的爱丽丝下午四点我在飞离洛杉矶的班机上试着去理顺生活我的青春散落在公路上就宿于各地旅馆，无数次登上头条我以一首歌成功立足吉他为伴我强烈渴望被接受和认可有名的人大多是被宠坏的孩子幻想使我们贪求无餍我猜想这就是我离开的原因我渴望活在现实里因此再见吧，梦游仙境的爱丽丝再见吧，黄砖路梦想和矫饰不同我没有找到天堂那不过是我孤独心灵的一种反映，我渴望找到生命中缺失的东西尽管尴尬，我不得不说一句摇滚乐界的陈词滥调当我的事业达到顶峰时，我的心情跌落到了谷底我从未想过，会是你让我心碎我以为你一定会爱我但你没有是的，如果心对爱饥渴它会产生幻觉，认假为真它甚至可以把妖魔认作下凡的天使你把我的爱练成一种武器然后把它像刀子一样捅向我你断了我最后一根心弦但你让我看清了现实因此再见吧，梦游仙境的爱丽丝再见吧，黄砖路梦想和矫饰不同你眼里的不是爱不过是我孤独心灵的一种反映，我努力寻找生命中缺失的东西成长不是梦想的消失而是一种能分辨你坚守的东西与导致你被骗的东西的能力并且梦想是个好东西因为它给生命带来新鲜事物但矫饰是一个终结它使谎言无限期地延续忘了你是谁只着眼于人们告诉你的那些东西真实比虚构更奇怪而这是我求取真实的机会没有那些漂亮的谎言，生活会变得更美好因此再见吧，梦游仙境的爱丽丝你可以留住你的黄砖路梦想和矫饰不同我眼里的不是泪水不过是我孤独心灵的一种反映，我意识到不过是我孤独心灵的一种反映，我意识到我已经找到了生命中缺失的东西

In iron-deficient cells, photosynthetic pigments content decreased, efficiency of photosynthesis was low, With the increase of the concentration of the iron-chelator 2, 2'-Dipyridyl in media, Fv/Fm decreased, indicating photoinhibition increased, the results were consistent with previous research; Heterocyst induction had one delayed time dependent on the status of iron-deficiency, More severe of the iron-deficiency condition, more late of the heterocyst induction, It is possible that certain iron-related genes may directly regulate heterocyst development; under different iron limitation conditions, Protein pattern were different, more serious of the iron-deficiency conditions, less bands of the protein pattern, We could not find the iron-deficiency induced protein; In general the toxic effects of ROS were potentiated by excess iron, But our results revealed that even under iron-deficiency conditions, Oxidative damage existed.

铁限制的细胞光合色素含量下降，光合作用能力下降，随着铁螯合剂2，2'-dipyridyl浓度的增加，叶绿素荧光F〓／F〓逐渐降低，显示光抑制变强。与前人的研究结果相似；依赖铁缺失状态，异形胞诱导有一段推迟的时间，铁缺失越严重，诱导越迟，推断某些铁相关的基因会直接调节异形胞发育；在不同铁营养条件下，蛋白质图谱的变化趋势很明显，缺铁越严重，则蛋白质谱带数愈少，但本文未能找到特异诱导蛋白；一般认为铁过量情况下ROS产生加强，本文结果显示即便在铁缺失条件下，也有氧化胁迫伤害，而且缺铁越严重，活性氧伤害越大。

Second, the emergence of collective actions is closely associated with the management incapability of government.

第二个观点是，群体性事件的大量产生，与当今政府的治理能力缺失存在很大关联。

To partially emphasize employment education deviate a school's work from the focus of training talents,and leads to such negative results as students' deficiency of creativity,maladaptation to the ...

片面强调"就业教育"会使学校工作偏离了人才培养这个重心，并导致学生创新能力缺失、适应职业岗位变化能力变差等不良后果。

This article in elaborated how develops in the brand strategyfoundation, discovered our country enterprise has the brand influenceinsufficiency, the innovation anergia in the brand strategydevelopment direction, cannot conquer the consumer, the financingability is limited and so on the question.

本文在阐述如何发展品牌策略的基础上，发现我国企业在品牌策略的发展方向存在着品牌影响力不足、创新能力缺失、不能征服消费者、融资能力有限等问题。

That means Pho85 kinaseand calcineurin were involved in salt tolerance with an identical target protein or in 中科院上海生化与细胞所博士学位论文摘要 the same pathway. Inhibition of calcineurin decrease the YPH499, pho80? mutant,and pap1 (pcl7)? mutant Mn2+ tolerance but not that of pho85? mutant and thepho85? mutant was more sensitive to Mn2+ than YPH499, pho80? mutant, and pap1(pcl7)? mutant even with the addition of cyclosporin A. Therefore, the conclusioncould be drawn that PHO85 gene played a dominant role in Mn2+ homeostasisregulation in compare with calcineurin. As for Ca2+ tolerance, cyclosporin A canincrease the tolerance to Ca2+ of all the mutant mention above, that means Pho85kinase and calcineurin function antagonistically in regulation of Ca2+ homeostasis.In Bioinformatics, BRI3 gene is a novel gene without any function clue but givehigh conservation in mammalian.

我们通过钙调磷酸酶的特异抑制剂环孢菌素A研究了YPH499、+2+pho85缺失株、pho80缺失株、pap1缺失株在钙调磷酸酶失活后对Na、Mn、2+Ca金属离子敏感性的变化，结果显示Pho85蛋白激酶和钙调磷酸酶通过直接+或间接激活同一个靶蛋白或途径来增强细胞对Na的耐受；和钙调磷酸酶相比，2+PHO85的缺失对酵母细胞Mn耐受性的破坏是相对控制性的，钙调磷酸酶的失2+活不能进一步降低pho85缺失株对Mn的耐受能力；Pho85和钙调磷酸酶在对2+Ca的耐受调节中是相互拮抗的，钙调磷酸酶的失活能增加pho85缺失株和2+pho80缺失株的Ca耐受。i中科院上海生化与细胞所博士学位论文摘要对本实验室克隆的人新基因BRI3进行系统的生物信息学分析，发现它是一个在哺乳动物中保守的，但功能未知的基因。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。