能分泌的

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Xanthomonas campestris pv. campestris is the causal agent of black rot disease of crucifers worldwide. In both plant and mammalian Gram-negative pathogenic bacteria, type III secretion systems, encoded by hrp cluster, play a crucial role in interactions with the host. The proteins assembled TTSS were highly conserved.

野油菜黄单胞菌野油菜致病变种是一种能在全球范围内引起所有十字花科植物黑腐病的重要病原细菌。hrp基因簇编码的Ⅲ型分泌系统在动植物病原细菌和寄主互作过程中起着极其重要的作用。

The coral polyp is a kind of coelenterate in the ocean, it, in order to pray on the tiny planktonic organism in the ocean as the food, can absorb calcium and carbon dioxide in the sea water in the course of growing, then secrete out the lime stone, turn into the outer cover that oneself survives.

珊瑚虫是海洋中的一种腔肠动物，它以捕食海洋里细小的浮游生物为食，在生长过程中能吸收海水中的钙和二氧化碳，然后分泌出石灰石，变为自己生存的外壳。

a cyanotype mi the gua fasten h2 is stop by body, have a strong nti- sour secrete a function.this text elaborateds its toxicity reaction from 8, though has many poison side effect, still don't lose to one safety stronger medicine, clinical with extensive application, want ～only full understanding, attention observation, ability decrease poison side effect reaction of occurrence.

甲氰咪胍系h2受体阻断剂，有强大的抗酸分泌作用。本文从八个方面阐述了其毒性反应，尽管有诸多毒副作用，仍不失为一安全性较强的药物，临床以广泛应用，只要充分了解，注意观察，能减少毒副作用反应的发生。

Globulus oil has the anti-inflammatory effect on chronic bronchitis induced by lipopolysaccharide in rats and the inhibitio effect on hypersecretion of airway mucins.

蓝桉油对脂多糖引起的大鼠慢性支气管炎具有一定的抗炎作用，并能抑制其气道黏蛋白高分泌现象。

And in sufficient sunlight, the body's adrenaline, thyroxin and the secretion of the title compound level will be improved, can effectively improve depression and other adverse psychological state.

而在充足的日照下，人体的肾上腺素、甲状腺素以及性腺素分泌水平都会有所提升，能有效改善情绪低落等不良心理状态。

Prostratin and the DPP as early as the 1990s were from the rain forests and arid regions of the two resin plant extract, when scientists discovered through research, Prostratin have two effects, first of all, it can to reduce the secretion of cells by HIV Body so that the AIDS infection rate of decline Secondly, it can be extracted hidden in immune cells within the AIDS virus.

Prostratin和DPP早在上世纪九十年代分别从雨林和干旱地区的两种树脂类植物中提取出来，当时科学家通过研究发现，Prostratin有两种功效，首先，它能促使体细胞减少分泌艾滋病病毒受体，使艾滋病感染率下降；其次，它可以逼出隐藏在免疫细胞内的艾滋病病毒。

VEGF can promote synthesis and secret of pulmonary surfactant protein B; antagonises under-regulation of expression of AEC II receptors by Dexamethasone.

VEGF能促进肺泡发育和肺表面活性物质结合蛋白B的合成、分泌，拮抗地塞米松下调肺泡Ⅱ型细胞受体的表达。

The increased ET -1 level in patients with EHF might be involved in development of acute renal failure.

在本实验中首次发现，EHFV体外感染HUVEC后，能刺激ET-1和PGI〓的分泌，这可能是EHF患者早期血浆ET-1和PGI〓升高的原因。

Just as the spit from the Gila Monster has provided a treatment for diabetes, now skin secretions from a South American ""shrinking"" frog could be used to treat type 2 diabetes also.

正像毒蜥可以作为糖尿病的一种治疗一样，现在，南美&皱缩&的青蛙皮肤分泌物也能用来治疗2型糖尿病

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。