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In this paper,the Lactate dehydrogenase, malate dehydrogenase,superoxide dis-mutase of five tissues of Carassius auratus gibelio; Cyprinus carpio Linnaeus; Esox americanus Gmelin; Leuciscus waleckii in Dalai Lake were studied by vertical polypropylene gel electrophoresis and the isozyme composion of four kinds of fishes were obtained. Then the analysis of the expressing specify of 3 kinds of isozyme in different tissues and loci were carried on and the genetic base of isozyme system was discussed.

本实验采用垂直板聚丙烯酰胺凝胶的电泳方法对达赉湖的野生鲤鱼、银鲫、狗鱼、东北雅罗鱼的五种组织脏器(肌肉、心脏、肝脏、脑、眼睛)的三种同工酶(苹果酸脱氢酶、乳酸脱氢酶、超氧化物岐化酶)进行了初步研究,获得了达赉湖四种鱼类物种的同工酶谱,在此基础上对同工酶在不同组织的表达差异和基因位点进行分析,探讨其同工酶系统的遗传基础。

The essence of deacetylation reaction of chitin was the hydrolysis of N-alkyl ethanamide.

甲壳质脱乙酰化反应其本质是N-烷基乙酰胺的水解。

Eamamined by fluorescin staining and Giemsa staining, 0.2 mg/L colcemid was considered suitable for inducing high percentage of micronuclei in A9 (neo12) cells, without causing death of a mass of cells.

通过荧光染色和吉姆萨染色分析,结果表明,A9(neo12)细胞经0.2 mg/L秋水仙素酰胺处理48 h后,89﹪的细胞产生微核化,每个细胞平均形成10个微核。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色,建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型,通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用;采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用;利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

The pegylated polylactide-poly glycolide nanoparticle, whose surface was co-modified withmaleimide-polyethylene glycol and methoxy-polyethylene glycol, was made by double-emulsion and solvent evaporation method andcovalently conjugated with thiolated lactoferrin via its maleimide functionalgroup.

第一章首先由复乳/溶媒蒸发法制备马来酰亚胺—聚乙二醇(Maleimide-PEG,MAL-PEG)和甲氧基聚乙二醇表面共修饰的聚乳酸-聚羟基乙酸纳米粒,然后通过其表面MAL-PEG与巯基化Lf共价连接而制备Lf-NP_。

In this dissertation, the sodium bentonite was modified using hexadecyl trimenthyl ammonium bromide and cationic polyacrylamide.

本文以市售钠基膨润土为原料,分别以十六烷基三甲基溴化铵和阳离子聚丙烯酰胺为改性剂,制各了新型复合插层有机膨润土。

The adsorption characteristics of p -nitrophenol in wastewater on honeycomb briquet slag modified by triethanolamine or hexadecyl trimethylamine bromide were studied.

利用三乙醇胺、溴化十六烷基三甲胺分别改性蜂窝煤渣,研究了改性煤渣吸附水中对硝基苯酚的性能。

The sodium bentonite was modified by organic reagent hexadecyl trimenthyl ammonium bromide and cationic polyacrylamide. The structures of modified organo-bentonite and the effect of concentration of the intercalating reagent and reacting time on decoloring capacity were studied.

用阳离子有机改性剂十六烷基三甲基溴化铵和阳离子聚丙烯酰胺复合插层改性钠基膨润土,分析了改性膨润土的结构,研究了改性剂质量浓度及二次改性时间对复合插层膨润土脱色效果的影响。

This prevented multiple mechanisms of biochemical dysfunction: activation of protein kinase C, activation of the hexosamine pathway, increased glycation and oxidative stress.

这可以阻止引起生化功能障碍的多重机制:蛋白激酶C的活化、己糖胺途径的激活、糖基化及氧化应激等。

As for its molecular mechanism, there are four hypotheses: glucose-induced activation of protein kinase C isoform; increased formation of glucose-derived advanced glycation end-products; increased glucose flux through the polyol pathway; and increased flux through hexosamine pathway.

对于其发病的分子机制,目前有四种假说:多元醇途径的增加;糖基化终末产物(advanced glycation end products,AGEs)的形成增加;蛋白激酶C(protein kinase C,PKC)的激活;己糖胺途径的增加。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

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