胸膜肺炎

Rabbits were inoculated with formaldehyde-inactivated bacteria suspensions of Actinobacillus pleuropneumoniaestrains of 12 serotypes with bee glue as an adjuvant.to prepare a set of monospecific antisera against each serotypes of APR.

将猪传染性胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae APP)全部12个血清型的国际参考菌株接种培养基大量培养，回收菌体、甲醛灭活后加入蜂胶佐剂制成疫苗，分别免疫健康的试验用白兔，获得了针对APP菌单一血清型的抗血清。

Objective To explore the cress immunoprotection of Actinobacillus pleuropeumoniaeg of various serotypes.

目的 探讨猪胸膜肺炎放线杆菌不同血清型间交叉免疫保护的关系。

By far, it happens in many regions of our country.15 serotypes of APP have been recognized by far. There is no or weak cross relation in different serotypes, which brings many troubles to diagnosis and prevention of this disease.

已发现的胸膜肺炎放线杆菌有15个血清型，不同血清型之间没有或仅有弱的交叉保护作用，这给临床诊断和免疫预防带来很大困难。

The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae， App)鞭毛蛋白编码基因的重组表达质粒，对其编码蛋白进行生物信息学分析，并将其在原核细胞中进行表达。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

With the pig breed and breath ask for virus integratedly (the cause of disease that prrsv) happening mixes or afterwards hair contracts is microbial, include a pig streptococcic, deputy pig is bloodsucking bacillus, pig is pneumonic a former put oneself in another's position, haemorrhage defeats courage and uprightness pleural and pausteur bacterium, pneumonic bloodsucking bacili and pig grippe virus.

和猪繁殖和呼吸综合征病毒发生混合或继发感染的病原微生物，包括猪链球菌、副猪嗜血杆菌、猪肺炎支原体、出血败血性巴斯德氏菌、胸膜肺炎嗜血杆菌和猪流行性感冒病毒。

Contagious ovine pleuropneumonia is a chronic respiratory infectious disease caused by Mycoplasma ovipneumoniae,which is one of primary sheep diseases and prevalent in many countries.

绵羊传染性胸膜肺炎是由绵羊肺炎支原体引起的一种绵羊慢性呼吸道传染病，在世界许多国家均有流行，为危害绵羊的主要传染病之一。

To construct an attenuate Actinobacillus pleuropneumoniae serovar 10 strain apxIC-/p36+ for new vaccine development.

构建血清10型胸膜肺炎放线杆菌弱毒菌株，为胸膜肺炎放线杆菌减毒活疫苗研究奠定基础。

PCP is one of important respiratory diseases in intensive pig farms, which continues to have a worldwide economic significance. The peracute or acute form may lead to a lot of pig death; the subacute or chronic form may also cause severe economic losses to the swine industry by lowering the speed of growth and the conversion efficiency of feed.

胸膜肺炎是规模化猪场最重要的呼吸道病之一，影响着全球的养猪业，最急性和急性胸膜肺炎可造成死亡，而亚急性和慢性病例则可导致猪生长缓慢，饲料报酬降低。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。