胸膜

Actinobacillus pleuropneumoniae is a very important respiratory pathogen for swine and causes great economic losses in pig industry worldwide.

胸膜肺炎放线杆菌是重要的猪呼吸道病原菌，给世界养猪业造成严重的经济损失。

There is no report about PCR method for detect APXIVA gene from tissue of swine. This research provided a new method for diagnosing actinobacillus pleuropneumoniae.

从猪血液、组织中直接提取DNA检测猪胸膜肺炎放线杆菌的APXⅣ基因，缩短了诊断该病的时间，在国内还未见报道。

Key words: swine; Actinobacillus pleuropneumoniae; PCR; clinical application

猪；胸膜肺炎放线杆菌；PCR；临床应用

The susceptible rates of 18 isolated Actinobacillus pleuropneumoniae strains to Florfenicol, Amikacin, Ceftriaxone were 77.78%, 83.33% and 83.33% respectively , which are the first to be chosen in control of PCP.

分离株对氟苯尼考、阿米卡星、头孢曲松敏感的比例分别为77.78％、83.33％和83.33％，它们可作为临床控制猪传染性胸膜肺炎的首选药物。

Following purification by Ni affinity chromatography and stepwise renaturation by dialysis, rB7-B5 showed sound antimicrobial activity in porcine actinobacillus pleuropneumoniae and antibiotics-resistant E. coli. The present study provides basis for the research and development of novel antibacterial preparations.

以经Ni亲和层析柱纯化和多步透析法复性的rB7-B5，对猪胸膜肺炎放线杆菌和耐药性大肠杆菌具有很好的抑菌活性，本研究为新型抗菌制剂的研制和开发奠定了基础。

To amplify transferrin-binding protein 2 (Tbp2) gene by PCR from Actinobacillus pleuropneumoniae, a pair of primers were designed according to sequence Z46774 of A.

参照猪传染性胸膜肺炎放线杆菌血清5型菌株序列Z46774设计1对特异性引物，用PCR方法扩增转铁结合蛋白2(Tbp2)基因，得到1条1624bp的片段，然后克隆到pMD18-T载体中，经测序比较，与参考序列的核苷酸同源性达99。

Key words: Actinobacillus pleuropneumoniae;OmlA gene;CPS gene;multiplex PCR;detection

胸膜肺炎放线杆菌；外膜蛋白基因；荚膜多糖基因；多重PCR；检测

Results In model group, alveolitis increases (much inflammatory cell infiltration), pulmonary interstitial fibroblast increases.

结果］模型组7d时肺泡腔内大量炎性细胞浸润，可见少量成纤维细胞，Ⅰ型细胞受损，Ⅱ型细胞明显增生；28d时肺泡炎减轻，肺间质和胸膜层胶原纤维明显增多。

Pulmonary embolism pain may be pleuritic when infarction of the lung results in pleuritis or may be anginal when right ventricular ischemia occurs secondary to sudden o et of pulmonary hyperte ion.

肺栓塞性疼痛可因肺梗死引起胸膜炎而呈胸膜性，也可因继发于突发性肺动脉高压的右心室缺血而呈绞痛样。

The right lung is atelectatic and floating in bloody fluid filling the right chest cavity as a consequence of trauma.

图示血胸。外伤后，大量出血，充满胸膜腔，使右肺萎陷，并在血性液体中飘浮。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。