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The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae, App)鞭毛蛋白编码基因的重组表达质粒,对其编码蛋白进行生物信息学分析,并将其在原核细胞中进行表达。

The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板,根据其鞭毛区与其它细菌高度同源性设计引物,利用PCR方法扩增鞭毛蛋白基因flic片段,将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定,再将其亚克隆与载体pGEx-kG分别双酶切和连接,构建重组表达载体pGEX-flic,并将其转化大肠杆菌工程菌BL21,进行原核表达。

HRCT still can show tubercle and lobar flocculus structure and the concern of its and pleura.

HRCT还可显示结节和肺小叶结构及其和胸膜的关系。

With the pig breed and breath ask for virus integratedly (the cause of disease that prrsv) happening mixes or afterwards hair contracts is microbial, include a pig streptococcic, deputy pig is bloodsucking bacillus, pig is pneumonic a former put oneself in another's position, haemorrhage defeats courage and uprightness pleural and pausteur bacterium, pneumonic bloodsucking bacili and pig grippe virus.

和猪繁殖和呼吸综合征病毒发生混合或继发感染的病原微生物,包括猪链球菌、副猪嗜血杆菌、猪肺炎支原体、出血败血性巴斯德氏菌、胸膜肺炎嗜血杆菌和猪流行性感冒病毒。

The concept of hemothorax, resource variation of pathophysiology and clinical manifestations of hematocele of pleural cavity; during treatment how to discriminate non-progressive ,progressive and coagulating hemothorax and adopt corresponding non-operative therapy and operative therapy .

血胸的概念,胸膜腔积血的来源,病理生理改变,临床表现。治疗必须区分非进行性,进行性血胸或凝固性血胸而采取非手术疗法及手术治疗。

In a supine position, pleural fluid collects in the posterior medial hemothorax and the dependent portion of the chest, and may present as a diffuse opacity on the affected side in the anteroposterior projection.

在一个仰位置,胸膜流体在后部中间hemothorax和胸口的依赖部分收集,并且也许提出作为在受影响的边的散开不透明在anteroposterior投射。

Hemothorax: accumulation of blood in the pleural cavity (the space between the lungs and the walls of the chest).

血胸:各种原因所致的胸膜腔内积血、肺萎陷称为血胸。

This is another sqamous cell carcinoma that extends from hilum to pleura.

这是肺鳞状细胞癌的另一实例,图示可见癌组织从肺门处扩展至胸膜处。

Nitrosamines, fiberglass, hydrocyanic acid, thorium oxide, beryllium and other pulmonary diseases (such as tuberculosis and chemical substances and lipid aspiration pneumonia), could lead to pleural mesothelioma.

亚硝胺、玻璃纤维、氢氰酸、氧化钍、铍及其它肺部疾病(如结核和化学物质及脂质吸入性肺炎),均可导致胸膜间皮瘤。

Abstract] Objective To investigate the characteristic CT findings of pleural indentation due to benign pulmonary lesions.

摘要] 目的研究观察肺内良性病变的胸膜凹陷的CT表现特征。

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