胸腺细胞

Objective To study the toxicity of fluoride on thymic epithelial cells and thymocytes , and to explore its mechanism.

目的 研究氟化物对胸腺上皮细胞的毒性作用及对胸腺细胞发育的影响，探讨其作用机制。

Objective To study the morphologic changes with age in thymocytes and to explore the effect of luteolin on the aging changes in thymocyte morphology,immunity and anti-oxidation activity in aged mice.

目的 探讨不同年龄胸腺细胞在胸腺内区域性分布的形态学变化，研究木犀草素对衰老大鼠胸腺形态学及免疫功能和抗氧化能力的影响。

The effect of F 1C on joint swelling and the immune respones of AA rats were evaluated by lymphocytotic reaction, flow cytometry, and ELISA. Results F 1C by ig administration to AA rats decreased the primary joint swelling of AA rats, stronger than TW and weaker than CsA, promoted the decreased splenocyte proliferation induced by Con A, and inhibited the augmented thymocyte proliferation induced by Con A in AA rats. The evaluation in vitro showed the F 1C inhibited the augmented thymocyte proliferation from AA rats and promoted the deficient IgG produced by splenocyte from AA rats.

结果 ig给予F1C(30mg／kg)对AA大鼠原发性足肿胀具有明显的治疗作用，该作用较TW强，较CsA弱；F-1C对AA大鼠低下的脾细胞增殖反应具有改善作用；对亢进的胸腺细胞增殖反应具有抑制作用。F-1C体外对AA大鼠低下的脾细胞产生IgG水平具有明显的促进作用，并能抑制亢进的胸腺细胞增殖反应。

We found that intravenously administered arginine significantly decreased the spleen weight, the serum interleukin-6 level, and IL-6 production by leukocytes, splenic macrophages, and thymocytes; significantly decreased tumor-necrosis factor-α production of leukocytes and thymocytes; and significantly increased the stimulating index of cell proliferation in T-thymocytes in peritonitic rats.

结果显示，静脉给予arginine显著降低腹膜炎大鼠脾脏重量、血清间白素-6、与白血球、脾脏巨噬细胞及胸腺细胞IL-6分泌量，且显著降低白血球与胸腺细胞肿瘤坏死因子-α分泌量，但显著增加胸腺细胞增生的刺激指数。

The protein had the same antigenicity as IgG, and the change of the protein had the same tendency as SHP-2, which was higher in canceration groups than in uncanceration groups and control groups in thymus cells, but there weren't significant change in bone marrow cells;⑧ The change law of the expression of SHP-1 and SHP-2 during γ-ray induced malignant transformation in BEAS-2B cell is that the expression of SHP-1 was increased synchronistically with malignant transformation of cell, and the expression of SHP-2 didn't have significant correlation with malignant transformation of cell.

癌变组胸腺细胞中SHP-2 mRNA编码PTPase催化区的700～1096bp间可能出现甲基化；⑦癌变组的胸腺细胞中存在一相对分子质量约为55×10〓的蛋白质，它与IgG具有相同的抗原性，其表达量的变化趋势与SHP-2的蛋白表达变化趋势基本一致，即在胸腺细胞中明显高于对照组及未癌变组，但在骨髓细胞中对照组高于癌变组；⑧SHP-1、SHP-2在γ射线诱发的BEAS-2B细胞恶性转化过程的变化规律：SHP-1蛋白表达量的升高与细胞的恶性转化在时间上基本一致，而SHP-2蛋白表达量与恶性转化无明显关系。

Besides, the splenocyte culture supernatant(IL-2) of Trionyx sinensis increased obviously thymocyte proliferation,which was also neutralized by polyclonal anti-human IL-2 antisera,and eliminated the suppressive effect of rabbit anti-thymocyte antisera on thymocyte.

中华鳖脾细胞培养上清液(含IL-2)对中华鳖胸腺细胞也有较明显的促增殖作用并能消除兔抗中华鳖胸腺细胞血清对中华鳖胸腺细胞增殖的抑制作用。

Results: 1. The value of all experimental indexes decreased significantly after heat stress, and reached the lowest at the time 2-8 hours after heat stress. This indicates that the immunity of the mice is damaged to the largest extent at 2-8 hours after heat stress. 2. L-arginine supplementation with appropriate dose could remit the acute atrophying of thymus and spleen tissue caused by heat stress. 3. After L-arginine supplementation with appropriate dose, the lymphocyte proliferation, the level of concentration of IL-2 and * expression of IL-2R raised in the group with room temperature; in the heat stress group the level of three indexs decrease significantly. Furthermore, the decrease of the group given 1 .Smglg.bw L-arginine is the smallest. This result indicates that the concentration of 1 .Smglg.bw L-arginine supplementation could remit the depressment of the immunity caused by heat stress. 4. The [Ca2~] in activated thymocytes of the group with L-arginine -2- supplementation is significantly higher than that of the group with water supplementation. This indicates that L-arginine supplementation could protect thymoeytes of mouse under heat stress. We also found that the fluorescence intensity of [Ca2~] in activated thymocytes of the group given 1 .Smglg.bw L-arginine is the highest.

结果：1、热应激后小鼠的各项免疫指标均有显著性下降，其中在2.8小时降到最低，提示在这个时间段小鼠的免疫功能受到最大损害；2、适量补充精氨酸有助于缓解热应激导致小鼠胸腺和脾脏的急性萎缩；3、常温组，补充适量精氨酸后小鼠淋巴细胞增殖活性、IL-2的浓度及IL-2R的表达均有显著性上升，而热应激后三指标均显著降低，其中在精氨酸给予浓度为1.5mg/g.bw时三指标的降低程度最少，提示该浓度可最大程度缓解热应激对小鼠免疫功能的抑制；4、补充精氨酸组的活化胸腺细胞［Ca~(2+)］较给水组有显著性上升，说明精氨酸对热应激小鼠胸腺细胞具有保护作用，并发现在精氨酸给予浓度为1.5mg/g.bw时活化胸腺细胞的胞浆钙离子荧光强度达到最大；5、热应激后小鼠血清NO的浓度均有不同程度的上升，补充精氨酸后血清NO上升更加明显，但并非随精氨酸给予浓度的增加而增加。

The thymuses of ZD rats appeared atrophy, with thin cortex, unclear boundary of thymus cortex and mudulla, and decreased thymocytes.

3显微镜下观察，ZD组大鼠胸腺明显萎缩，皮质变薄、疏松，皮、髓质分界模糊，胸腺细胞明显减少，胸腺小体的数量也明显减少。补锌后，ZS组胸腺的病理结构有明显恢复，与ZA〓组无明显差异。

The result indicated different expression of CD3,CD4,CD8 on large thymocytes and small thymocytes.

结果表明，胸腺中大胸腺细胞和小胸腺细胞的CD3、CD4及CD8的表达不同，在大胸腺细胞中，CD3的表达比小胸腺细胞的CD3 表达要高出10个百分点。

Fas and Fas-L are both important molecules which mediate heat stress-induced apoptosis of thymocytes in vitro.

HPS70是热应激胸腺细胞损伤的标志，Fas与Fas-L是介导热应激诱导胸腺细胞凋亡的重要分子。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。