胸腺的

Objective To study the morphologic changes with age in thymocytes and to explore the effect of luteolin on the aging changes in thymocyte morphology,immunity and anti-oxidation activity in aged mice.

目的 探讨不同年龄胸腺细胞在胸腺内区域性分布的形态学变化，研究木犀草素对衰老大鼠胸腺形态学及免疫功能和抗氧化能力的影响。

In PsNPV infected treatment, the color of the gland was purple-black dyed with eosin and the cells were distortedly squeezed together. The tracheae nearby showed grievous pathological changes and a lot of white granules deposited around it. The pathological changes in PsGV infected treatment showed another pattern. The gland body was relative small with smaller individual cells, most of which were red dyed with eosin. The cell limits were obscure.In coinfected treatment,there were few gland cells colorated by esoin.

结果表明，不同感染组粘虫前胸腺腺体都有不同程度的组织病变，PsNPV感染组在感染晚期与前胸腺相连的气管严重病变，出现大量白色颗粒状物累积，被伊红染成紫黑色，腺体细胞被挤压变形；PsGV感染组的前胸腺腺体变小，单个细胞也小，细胞界限不十分明显；两种病毒混合感染组的腺体细胞小，能被伊红染色的细胞极少。

The results showed : thymus, Bursa, Harderian gland and spleen weight increase with age, gradually increasing; Thymus index, Bursa index and Harderian gland, the index increased with age gradually and the spleen index increase with age in the first increased and then decreased.

结果表明：胸腺、法氏囊、哈德氏腺和脾脏的重量随日龄的增长，逐渐增大；胸腺指数、法氏囊指数和哈德氏腺指数随日龄的增长逐渐变小，脾脏指数随日龄的增长，先增加后减小。

In the article, we took part sample out of 158 patients to exam the expression level of Bcl-2 and Fas in myasthenic thymus tissue, and to find the role of which in whole pathogenesis. we also try to find the role of neopterin and sIL-2R through examing the concentration of neopterin and sIL-2R in peripheral blood of 26 MG patients. Material and methods1.select of case: to choose 158 patients who wear followed up from 1988 to 2002 after operation. All patients were diagnosed as MG, and treated with same medical cure during period of preoperation and post operation. 2.Collectionofdata:.To collect all data through designing a uniform follow-up table for thymectomized patients ,and to analysis the effect of factors including age, gender, disease duration of preoperation, clinic types and pathological types to prognosis at three follow-up period?

本文拟在对全组病例的远期预后进行流行病学统计分析的基础上，通过抽取一定量样本，研究重症肌无力患者胸腺组织中伽 12、Fas的异常表达及生发中心的形成在重症肌无力发病机制中可能的作用，以及它们对胸腺切除术后远期预后的影响；与之相似，通过检测部分样本外周血清中新碟吟和可溶性IL－2受体的浓度及其与正常对照组的差异，以期推测两者在重症肌无力起病及病情进展中的作用和意义，并分析其对胸腺切除术后远期预后的影响。

The biological behavior of thymoma with or without capsule might not be assessment standard for benign or malignancy.

有包膜的胸腺瘤和无包膜的胸腺瘤的生物学行为不是判断胸腺瘤良恶性的最佳指标。

The hepatocytes which were injected into thymus could survive more than 2 weeks after injection and appeared to be irregular hepatic cells cords and sinusoid in shape, and severe vacuolar degeneration in 3 weeks.

本实验在未用任何免疫抑制剂的情况下，肝细胞胸腺内注入后1周、2周，显微镜下见植入胸腺内的肝细胞已生长出不规则的肝索及肝窦样结构。3周时的胸腺内植入的肝细胞仍存活，但空泡变性明显。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

RESULTS The results indicated that PCF could significantly increase the viability of thymocytes and enhance the activities of SOD, GSH-Px, CAT in cells. PCF decreased the amounts of ROS and the rate of apoptosis in cells. PCF inhibited the morpholog alteration of the thymocytes irradiated by UVB and maintained the ultrastructure of cells in normal. PCF attenuated UVB caused DNA fragmentation in thymocytes. While combined ERKs inhibitor yielded typical DNA fragmentation, co-treatment of thymocytes with PCF and JNKs and p38 kinase inhibitors completely blocked UVB-induced DNA fragmentation.

结果 PCF能显著提高UVB辐射后小鼠胸腺淋巴细胞的活性；提高胞浆中SOD、GSH-px、CAT的活性，降低ROS含量及细胞凋亡率；抑制UVB辐射所致的胸腺淋巴细胞凋亡的形态学改变，维持细胞的正常超微结构；抑制UVB所致的胸腺淋巴细胞DNA片段的出现；预先应用ERK抑制剂使DNA片段增加，给予JNK和P38抑制剂后协同PCF使DNA片段带减少。

The numbers of ANAE(superscript +) T cells in the spleen and head kidney were more than those of thymus, therefore, it is suggested that the matured T cells are emigrated thymus to spleen and head kidney.

实验结果进一步证明了胸腺中的T细胞多为不成熟的T细胞，此外，还含有大量的IgM细胞；而脾脏、头肾内的ANAE T细胞多于胸腺，推测胸腺成熟的T淋巴细胞迁移到头肾和脾脏。

To study the effects on the body of animals after incorporation of 3H-TdR and its mechanism, the laws of distribution A retention and absorbed dose in blood , thymus, spleen, femur, heart, lung,kidney, liver, brain , spermary , small intestine and muscle were detected by liquid scintillation counter after 3H-TdR was injected into the tail vein of mouse; the process of dynamic incorporation of in the lymphocytic cell nucleus of blood , thymus , spleen and the nucleus of femur was monitored; the absorbed dose in the cell nucleus was estimated; the influence to RNA"s synthesizing and cell"s survival was observed using the technology of CLSM; A segment of Egr-1 gene was cloned with reverse transcriptase- polymerase chained reaction, the influence to the expression of Egr-1 gene in the spleen cells was observed.

为了探讨有机结合氚内污染对生物机体的影响及其作用机理，本课题应用均相液体闪烁测量技术研究了氚标记胸腺嘧啶核苷(~3H-TdR)经尾静脉注入小鼠机体后，在血液、脑、胸腺、心脏、肺脏、肝脏、脾脏、肾脏、小肠、睾丸、肌肉和股骨共12种组织器官的生物动力学分布规律及其吸收剂量；应用纸片法液体闪烁测量技术研究了~3H-TdR内污染在外周血、胸腺和脾脏淋巴细胞及骨髓细胞的动态滞留规律，估算了细胞核的吸收剂量；应用共聚焦显微镜观察了~3H-TdR内污染对脾细胞核酸合成及其存活率的作用，应用反转录PCR技术研究了~3H-TdR内污染对脾细胞早期生长反应基因-1(Egr-1)转录表达的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。