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In order to mimic this repair process of apoenzyme, we synthesized two model compounds, in which tryptophan and/or tyrosine was covalently linked to cyclobutane thymine dimer, and measured the splitting quantum yield of dimer under 295 nm light. By investigating the photosensitized splitting properties of the dimer, some new insights into the intramolecular electron-transfer process were gained.

为了模拟酶蛋白的这一修复过程,合成了色氨酸和/或酪氨酸与胸腺嘧啶二聚体共价连接的化合物,作为酶-底物复合物的模型,研究了它们在295 nm光照射下氨基酸残基光敏化二聚体裂解的性质,测定了二聚体裂解量子产率,获得一些新的结果并对其进行了分析。

By using the assessment method of health care,the content of serum lysozyme,the thymus index,spleen index,the phagocytic function of the macrophage and the delayed type hypersensitivity were tested to analyse the immunoregulatory function of Hericium erinaceus polysaccharide.

应用保健功能的评价方法,通过对血清溶菌酶含量、胸腺指数与脾指数、腹腔巨嗜细胞吞噬功能的测定以及迟发型超敏反应,来定性分析猴头菌丝多糖的免疫调节作用;通过对血清SOD、CAT、MDA含量的测定,来分析猴头菌丝多糖的抗氧化功能。

Methods 1. The mice were divided into control and 3 Ganodermalucidum spores oil groups (at low, moderate and high doses), orally given forconsecutive 30 days. The body, spleen, thymus weight were observed, study on theeffect of the drug on the cellular immune function by proliferation and transformationof spleen lymphocytes test; study on the effect of the drug on the humoral immunefunction of mice by serum erythrocytolysin test; study on the effect of the drug on themononuclear macrophage function by carbon clearance and neutral red test.

实验方法 1设玉米油对照组和低、中、高三个灵芝孢子油剂量组,连续灌胃30天后观察小鼠体重、胸腺、脾脏重量指数;采用吞噬中性红和碳廓清实验测定对小鼠单核-巨噬细胞功能的影响;血清溶血素实验测定对小鼠体液免疫功能的影响;ConA诱导脾淋巴细胞转化实验测定对小鼠细胞免疫功能的影响;MTT法测定对小鼠NK细胞杀伤活性、脾细胞产生TNF-α和IL-2含量的影响。

Besides as nucleus protein, ProTαparticipate in cell multiplication and it could apply to knubbly forecasting, diagnosis and treatment, which is studied by scientists mainly.

由于在免疫和细胞生长调控方面的广泛作用,ProTα在其被发现后的20多年里,一直作为胸腺素家族中的研究重点而备受人们的关注。

The result fights allergic experiment to show, dispel the wind stops land of can different level restrains urticant Ding allergy of akin and passive skin mixes the bandicoot that antiserum causes 2, the sex of small rat late hair of chloric benzene be caused by exceeds 2 nitro- of 4- quick the thymus index that reaction; still can increase small rat when dosage greatly and lienal index; stop urticant experiment shows, dispel the wind stops urticant Ding can reduce the time of fit of small rat itching with dextrose lead anhydride and itching duration apparently, can increase Guinea pig to be able to bear or endure those who suffer phosphoric acid histamine send urticant threshold.

结果抗过敏试验显示,祛风止痒酊能不同程度地抑制抗血清诱发的大鼠同种被动皮肤过敏和2,4-二硝基氯苯所致的小鼠迟发性超敏反应;大剂量时还可增加小鼠的胸腺指数及脾指数;止痒试验显示,祛风止痒酊能明显降低右旋糖酐诱导的小鼠搔痒发作次数及搔痒持续时间,并能增加豚鼠耐受磷酸组胺的致痒阈。

Each group of rats was kept in a separate cage. All of the rats drank water freely and were fed on stand food for rats.The experiment lasted 4 weeks. Indexes to be examined are as follows:⑴spleen T lymphocyte transform value,⑵spleen NK cell activation alternation value,⑶abdominal cavity macrophagocyte IL-1 and IL-2 activation alternation value.

所有动物分笼饲养,各组均自由饮水,喂食标准鼠类饲料,实验时间为4周。4周后,ME组和FE组作一次性力竭游泳运动后和其他各组大鼠股动脉放血处死,留取血清,无菌取胸腺和脾脏,行以下指标检测:⑴脾脏T淋巴细胞转化值;⑵脾脏NK细胞毒活性的变化值;⑶腹腔巨噬细胞IL-1的活性变化值和脾脏分泌的IL-2的活性变化值。

And it could help to reduce the resistance of iron to zinc and enhance both the zinc and iron in crural bone and could enhance calcium and zinc in pygal muscle of mice. 4 Effects of LBP on obesity LBP could cure obese mice induced by monosodium glutamate.

连续灌胃5mg·kg〓·d〓、10mg·kg〓·d〓和20mg·kg·d〓的LBP-4三周后可显著减少雌性幼鼠的体重增加量,提高食物转化率;显著提高小鼠的耐力;减少小鼠体内的脂肪含量;增大胸腺脂数,降低肝脏指数和脾脏指数。

Thymosin 1(Tα1) is a acidic protein composed of 28 amino acid residues with isoelectric point 4.2 and molecular weight 3102.Its amino acid sequence is: N-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu -Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-C. T1 is a T-cell potentiating factor.

胸腺素α1(thymosin 1,Tα1)是由28个氨基酸组成的酸性多肽,等电点4.2,相对分子量3108,其氨基酸序列为:N-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-C[1,2]。Tα1具有多种免疫活性,可以促进T淋巴细胞的成熟和分化,促进成熟的T细胞、自然杀伤细胞分泌各种细胞因子如IL-2、INF-α等,同时也促使高亲和力白介素-2受体的生成[3-10]。

During the first two weeks after birth, the growth performances were not significant between the mutant mice and the normal ones, but after that, the mutant mice grew much slower. The differences were significant in the indexes of eyes, lungs, thymuses, and adrenal glands between the genders. At the same time, the differences were significant in the organ indexes, such as lungs, adrenal glands, uterine and testicles between the normal phenotype mutant mice and the abnormal ones. And these two kinds of mice showed different amount of hemoglobin and red blood cell.

结果:B6-Co小鼠不育率22%、平均产仔数5.62±2.55只、离乳成活率56.59±41.22显著低于正常的B6小鼠,而胎间隔29.50±10.50天比正常B6小鼠短。B6-Co小鼠出生后2周内生长发育与正常B6小鼠基本一致,2周后,其生长发育慢于正常B6小鼠。B6-Co小鼠雌雄间的眼球、肺、胸腺、肾上腺的脏器指数差异有统计学意义。B6-Co表型异常小鼠与B6-Co表型正常小鼠相比,肺、肾上腺、子宫、睾丸的脏器系数差异有统计学意义。B6-Co小鼠表型异常小鼠在血红蛋白和红细胞数量上异于B6-Co表型正常小鼠。

Results: B6-Co mice showed higher sterile rate, and significant lower average litter size and survival rate at weaning than the normal B6 mice, but the time interval between deliveries was shorter in the mutant population. During the first two weeks after birth, the growth performances were not significant between the mutant mice and the normal ones, but after that, the mutant mice grew much slower. The differences were significant in the indexes of eyes, lungs, thymuses, and adrenal glands between the genders. At the same time, the differences were significant in the organ indexes, such as lungs, adrenal glands, uterine and testicles between the normal phenotype mutant mice and the abnormal ones. And these two kinds of mice showed different amount of hemoglobin and red blood cell.

结果:B6-Co小鼠不育率22%、平均产仔数5.62±2.55只、离乳成活率56.59±41.22显著低于正常的B6小鼠,而胎间隔29.50±10.50天比正常B6小鼠短。B6-Co小鼠出生后2周内生长发育与正常B6小鼠基本一致,2周后,其生长发育慢于正常B6小鼠。B6-Co小鼠雌雄间的眼球、肺、胸腺、肾上腺的脏器指数差异有统计学意义。B6-Co表型异常小鼠与B6-Co表型正常小鼠相比,肺、肾上腺、子宫、睾丸的脏器系数差异有统计学意义。B6-Co小鼠表型异常小鼠在血红蛋白和红细胞数量上异于B6-Co表型正常小鼠。

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