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After 10 days, 20 days and 30 days for grass carp (Ctenpharyngoden idellus Cuvier et Valencinnes) immunized by being injected Aeromonas hydrophilia, the ANAE (acid α-naphthyl acetate esterase) positive cells ratioes increased, and the same time it was found that the ACP activity increased up too, it showed that the fish bodies were being at immune responding situation and their immnue activities enhanced.

通过对草鱼免疫器官的六种同工酶的分析发现:LDH在血浆、血液细胞、脾脏和胸腺中都表达为5条区带,前肾还额外显示出X酶带,血浆只有3条酶带;ADH除了胸腺为一条带以外,其它的均为3条酶带;MDH和3-GPDH在这些组织中均表达为3条酶带;EST在前肾中为7条酶带,其余的都为6条酶带;POX只在前肾和脾脏中表达为2条酶带。

RokoszAdenine, along with guanine, thymine and cytosine, makes up DNA structures and is responsible for helping the body metabolize energy.

腺嘌呤,随着鸟嘌呤,胸腺嘧啶和胞嘧啶,构成了DNA的结构,并帮助身体能量代谢负责。

The most abundant bases are cytosine, thiamine, and uracil and adenine and guanine.

最丰富的碱基是胞嘧啶,胸腺嘧啶,尿嘧啶,腺嘌呤和鸟嘌呤。

DNA consists of a chain made from four types of nucleotide subunits: adenine, cytosine, guanine, and thymine.

的DNA链组成一个由4种核苷酸亚基:腺嘌呤,胞嘧啶,鸟嘌呤和胸腺嘧啶。

The DFT (B3LYP/6-31 + G) method was used to study the relative stabilities and probable proton transfer in isolated and monohydrated 2,4-dithiothymine molecules in gas phase. The effects of water on relative stabilities and the rate of the proton transfer were analysed. The results indicate that only one tautomer for 2,4-dithiothymine can exist in gas phase.

采用密度泛函方法在B3LYP/6-31+G水平上研究了2,4-二硫基胸腺嘧啶孤立分子和水合物的异构体的相对稳定性和可能的质子迁移反应,分析了水分子的参与对 2,4-二硫基胸腺嘧啶异构体的相对稳定性和质子迁移速率的影响。

The results of this study indicated that there were changes on the distribution of T-lymphocyte subsets in patients with asthenia gravis.

MG患者存在有明显的T细胞亚群的改变,符合自身免疫性疾病的特点:胸腺切除手术近期对患者周围血中各淋巴亚群无明显影响;胸腺切除围手术期使用TP-5治疗对其发挥免疫调节作用的主要机理之一。

Compared with control,①the mean tumor weight of H22 of SXKA Granules three dose groups were decreased significantly(P.01,P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 30%;②the mean tumor weight of S180 of SXKA Granules three dose groups were decreased significantly(P.01),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;③the mean tumor weight of EAC of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20 g/kg dose groups were above 38%;④the mean tumor weight of Lewis carcinoma of SXKA Granules three dose groups were decreased significantly(P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10、5 g/kg dose groups were above 36%;⑤the mean tumor weight of W256 of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;⑵Compared with control,SXKA Granules 20、10 g/kg dose groups had extended the survial time of the P388-bearing mice respectively(P.01),and the mean prolong rates of SXKA Granules 20、10 g/kg dose groups were above 50%;⑶Compared with S180-bearing group, SXKA Granules 20、10 g/kg dose groups could increase the weight of thymus and spleen, Spleen index and thymus index were increased, SXKA Granules 5 g/kg dose group could increase thymus index(P.05);⑷As Compared with control group, SXKA Granules 20、10 g/kg dose groups could improve mouse serum half hemolysis value depressed by transplanted tumor dramatically(P.01), which revealed the SXKA granules could improve the mouse humoral immunity system;⑸SXKA Granules 20 g/kg dose group could increase of englobe indexαon S180-bearing mice remarkably(P.01), which indicated the SXKA Granules could improve their cellular immunity system.

对荷W256大鼠,生兴克癌冲剂20、10、5 g / kg三组的平均瘤重明显低于对照组(P.01,P.05),生兴克癌冲剂20、10 g / kg组的平均肿瘤抑制率均大于32 %;⑵与空白对照组相比,生兴克癌冲剂20、10 g/ kg能显著地延长移植小鼠白血病P388小鼠的存活天数(P.01),生兴克癌冲剂20、10 g/ kg对荷白血病P388小鼠生命延长率均在50%以上;⑶与S180荷瘤组相比,生兴克癌冲剂对荷瘤鼠的免疫器官重量、胸腺指数和脾指数有一定的提高趋势,其中生兴克癌冲剂5 g / kg组对荷瘤小鼠的胸腺指数有一定的提高作用(P.05);⑷与S180荷瘤组相比,生兴克癌冲剂20、10 g /kg组可提高由荷瘤引起的小鼠血清半数溶血素值的降低(P.01),表明其可提高荷瘤小鼠体液免疫功能;⑸与对照组相比,生兴克癌冲剂20 g /kg组可提高荷S180肉瘤小鼠的免疫吞噬系数α值(P.01),表明其可提高荷瘤小鼠细胞免疫功能。

Since hyperplastic changes were seen in 35% of MuSK-negatie patients, the thymus could be inoled in some of these cases.

然而在35%的MuSK抗体阴性的病人中可见明显胸腺增生,提示胸腺在此类病人的致病机制中有一定的作用。

Results: 1. The value of all experimental indexes decreased significantly after heat stress, and reached the lowest at the time 2-8 hours after heat stress. This indicates that the immunity of the mice is damaged to the largest extent at 2-8 hours after heat stress. 2. L-arginine supplementation with appropriate dose could remit the acute atrophying of thymus and spleen tissue caused by heat stress. 3. After L-arginine supplementation with appropriate dose, the lymphocyte proliferation, the level of concentration of IL-2 and * expression of IL-2R raised in the group with room temperature; in the heat stress group the level of three indexs decrease significantly. Furthermore, the decrease of the group given 1 .Smglg.bw L-arginine is the smallest. This result indicates that the concentration of 1 .Smglg.bw L-arginine supplementation could remit the depressment of the immunity caused by heat stress. 4. The [Ca2~] in activated thymocytes of the group with L-arginine -2- supplementation is significantly higher than that of the group with water supplementation. This indicates that L-arginine supplementation could protect thymoeytes of mouse under heat stress. We also found that the fluorescence intensity of [Ca2~] in activated thymocytes of the group given 1 .Smglg.bw L-arginine is the highest.

结果:1、热应激后小鼠的各项免疫指标均有显著性下降,其中在2.8小时降到最低,提示在这个时间段小鼠的免疫功能受到最大损害;2、适量补充精氨酸有助于缓解热应激导致小鼠胸腺和脾脏的急性萎缩;3、常温组,补充适量精氨酸后小鼠淋巴细胞增殖活性、IL-2的浓度及IL-2R的表达均有显著性上升,而热应激后三指标均显著降低,其中在精氨酸给予浓度为1.5mg/g.bw时三指标的降低程度最少,提示该浓度可最大程度缓解热应激对小鼠免疫功能的抑制;4、补充精氨酸组的活化胸腺细胞[Ca~(2+)]较给水组有显著性上升,说明精氨酸对热应激小鼠胸腺细胞具有保护作用,并发现在精氨酸给予浓度为1.5mg/g.bw时活化胸腺细胞的胞浆钙离子荧光强度达到最大;5、热应激后小鼠血清NO的浓度均有不同程度的上升,补充精氨酸后血清NO上升更加明显,但并非随精氨酸给予浓度的增加而增加。

With the methods of Reverse transcription-PCR and cDNA sequencing, the expression of FHIT gene was detected in radiation carcinogenesis. The results show that: 1, The FHIT gene's sequence in normal BALB/c mice compare with the sequence of mice in Ge neBank which absents exon 3, but the function of FHIT protien doesn't alter.

结果发现:1、正常BALB/c 小鼠的FHIT基因序列同GeneBank中小鼠的FHIT基因序列比较缺少了外显子 3,但其表达的蛋白功能并未发生改变;2、FHIT基因在辐射损伤与辐射致癌的早期过程中,对照组血液、胸腺及骨髓均未出现异常的FHIT转录本的表达,不同剂量照射组中血液、胸腺及骨髓均有部分样品出现分子量较小的异常FHIT 转录本的表达。

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