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The EAE rats were induced by injecting subcutaneously an emulsion of GPSCH and CFA. In addition, each rat received an intracutaneous injection of pertussis. Albumin in serum and CSF samples was examined on the days of 4,6,8,10,12,14,16,18 and 20 p.i.. The integrity of BBB was assessed by caculating CSF to serum albumin quotient. At the same time the brains, spinal cords, thymus and inguinal lymph nodes were removed for paraffin sections cutting. These sections were used for HE staining and ICAM-1 semiquantitative immunohistochemical staining. The results showed as follows:1. None of EAE rats on days 4,6 and 8 p.i. showed clinical signs whichbecame apparent on day 10 p.i.(2.33±0.76; n=6). On days 12 and 14 p.i.

检测p.i。第4天、6天、8天、10天、12天、14天、16天、18天、20天血清和CSF中ALB含量,其CSF/S ALB比值作为评价BBB损害的指标;同时段取动物脑、脊髓、胸腺和腹股沟淋巴结制成石蜡切片行HE染色和ICAM-1半定量免疫组化分析,结果显示如下: 1、EAE大鼠p.i.4-8天无任何临床表现;p.i.10天症状开始明显,症状评分为2.33±0.76(n=6);p.i.12-14天为发病高峰期,症状评分为3.75±0.54(n=12),病情由最初的活动减少,体重减轻逐渐发展为肢体无力、瘫痪,共济失调,甚至濒死状态;p.i.16天大部分动物症状开始缓解,评分为2.08±0.66(n=6);p.i.20天疾病症状完全消失。

In the whole rats, the following tissues or organs were extracted and fixed in the most appropriated fixator (Bouin liquid or Formalin at 10%) then included in paraffin blocks : all the organs showing significant macroscopic lesions, encephalon (brain hemisphere, cerebellum and annular protuberance), spinal cord, sciatic nerve, salivary glands, esophagus, stomach, duodenum, jejunum, ileon, caecum and colon, rectum, liver, spleen, bone marrow, lymph node close to and at distance, thymus, heart, aorta, trachea, lungs, thyroid/parathyroid, surrenals, pancreas, hypophysis, kidney, bladder, gonad, uterus, mammary glands, epididymis, prostate, eyes, lachrymal glands, bones, skin, thigh muscle and implantation sites (2 per animal).

从整体大鼠内,下列组织或器官提取后用最适宜的固化剂( Bouin 液或10%的甲醛溶液)进行固定,然后包入石蜡块内:所有显示有明显肉眼可见损害的器官、脑髓、脊髓、坐骨神经、唾液腺、食道、胃、十二指肠、空肠、回肠、盲肠、大肠、直肠、肝脏、脾、骨髓、近端和远端淋巴结、胸腺、心脏、主动脉、气管、肺、甲状腺/甲状旁腺、肾上腺、胰腺、垂体、肾、膀胱、性腺、子宫、乳腺、附睾、前列腺、眼睛、泪腺、骨骼、皮肤、股肌以及假体植入部位(每只动物2处)。

The control tissues were negative.Four 45-day-old SPF pigs infected with PRRSV BJ-4 isolate and four stillborn fetuses were deteced with the probe.Positive hybridization signals could be detected in lung,tonsil,lymph nodes,thymus gland,brain,etc.

应用探针对人工感染PRRSV北京分离株BJ-4的4头SPF猪及4例死胎的组织进行了检测,均可在肺门淋巴结、胸腺、脑、扁桃体等组织中发现阳性杂交信号。

Human have different hemopoietic organs at different times, such as fetal liver, thymus, lymph node and bone marrow, etc.

人类不同的发育时期有不同的造血组织,如胎肝、胸腺、淋巴结、骨髓等。

T lymphocyte percentages of peripheral blood, lymph node, spleen, thymus and bone marrow of maters of interspecific, intraspecific embryo implantation and pseudopregnant mice at corresponding time were measured using fluorescence labeled monoclonal antibody and flow cytometry.

利用荧光标记的单克隆抗体染色结合流式细胞术,检测种间、同种胚胎移植以及同时期假孕母体外周血、淋巴结、脾脏、胸腺、骨髓中T淋巴细胞的百分率。

"The most highly organized components are the thymus and lymph nodes, and the least organized are the cells that wander in the loose connective-tissue spaces under membranes lining most body systems, where they can establish lymph nodules (local lymphocyte production centre) in response to antigens."

其中结构最为完备的是胸腺和淋巴结。结构最小的是分散在衬接多数身体系统的黏膜下的疏松结缔组织里,它们在这里对抗原做出反应而形成淋巴小结(局部的淋巴球细胞生发中心)。

"The most highly organized components are the thymus and lymph node s, and the least organized are the cells that wander in the loose connective-tissue spaces under membranes lining most body systems, where they can establish lymph nodules (local lymphocyte production centre) in response to antigens."

其中结构最为完备的是胸腺和淋巴结。结构最小的是分散在衬接多数身体系统的黏膜下的疏松结缔组织里,它们在这里对抗原做出反应而形成淋巴小结(局部的淋巴球细胞生发中心)。

"The most highly organized components are the thymus and lymph nodes, and the least organized are the cells that wander in the loose connective-tissue spaces under membrane s lining most body systems, where they can establish lymph nodules (local lymphocyte production centre) in response to antigens."

其中结构最为完备的是胸腺和淋巴结。结构最小的是分散在衬接多数身体系统的黏膜下的疏松结缔组织里,它们在这里对抗原做出反应而形成淋巴小结(局部的淋巴球细胞生发中心)。

The invasion and metastasis occurring in footpads and lymph nodes were observed and computed, the expression of VEGF, MVD were also me asured with immunohistochmeistry staining.

结果 麝黄消瘤方治疗组与模型组相比,其局部瘤组织的侵袭和腘窝淋巴结转移程度及淋巴结指数有降低趋势,其中大剂量组有显著性差异(P<0.05);大中剂量组可使胸腺指数明显升高(P<0.05)。

After inoculation of leukemia virus,the changes in ANAE,AcP and β-Gase reactions were observed in the thymocyte and thymie nurse cell in thymus cortex of experimental mice.

对接种白血病病毒后30d、75d 小鼠胸腺和淋巴结的组织结构及酸性非特异性酯酶、酸性磷酸酶、β~葡糖苷酸酶反应类型进行了观察,并与正常组作对照。

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