胶骨

Objective To investigate bio characteristics of bone stromal cells in different concentrations of alginate combined with xenograft.

目的 探讨不同浓度的海藻酸盐凝胶与去抗原异种骨构建组织工程载体的生物学性能。

Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment.

试验采用24h内昆明乳鼠，拉颈处死，无菌取出头盖骨，在超净工作台内将其剪碎，先用0.25%胰蛋白酶37℃消化30min，然后用0.2%胶原酶37℃振荡消化60min，反复吹打骨片分离更多细胞。

Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment. The bone pieces were digested by 0.25% trypsin for 30 min at 37℃ at first, then digested by 0.2% collagenase at 37℃ in constant temperature oscillator for 60 min, and rinsed repeatedly to disaggregate more single-cells.

试验采用24h内昆明乳鼠，拉颈处死，无菌取出头盖骨，在超净工作台内将其剪碎，先用0.25%胰蛋白酶37℃消化30min，然后用0.2%胶原酶37℃振荡消化60min，反复吹打骨片分离更多细胞。

In experimental group, a bone hole was made by using a diamond-tipped burr at the following coordinates: anterior 1mm from bregma and lateral 3mm relative to the midsagittal sinus, a 10ul solution containing 106 C6 gliomaous cells was stereotactically implanted into right caudate nuclei to establish brain glioma model.

于前囟前1mm、矢状缝右旁开3mm处用牙钻钻一骨孔，用静脉注射套管针立体定向注射10ul含有约106个C6细胞的混悬液于鼠脑右侧尾状核内，建立鼠脑胶质瘤模型。

Objective To investigate the feasibility of biological fibrin glue/cancellous bone matrix as scaffold for chondrocyte.

目的 探讨生物蛋白胶，松质骨基质作为软骨细胞培养支架的可行性。

Signal intensity on T2-weighted depends on the amount and degree of bony trabeculae, cellularity and collagen.

在T2WI所呈现的信号特点依赖于骨小梁、细胞成份及胶原纤维的多少。

Hydrophobically modified anion polyelectrolytes based on Fenugreek gumwere prepared by fixation of dodecylor cetylchains onto carboxymethyl Fenugreek gum backbone via ester functions.

将十二烷基与十六烷基以酯键方式分别引入经羧甲基化改性的香豆胶大分子骨架上，制备了基于香豆胶的疏水改性阴离子聚电解质衍生物。

RESULTS: Light microscope showed that the collagen fibers arranged regularly in AECM with blankness of bone lacunas by HE, Mallory-Heidenhain rapid one-step dyeing and that the region around bone lacunas was stained different degrees of blue-green by Alcian blue dyeing.

结果 HE、Mallory- Heidenhain氏快速一步法染色后镜下可见AECM胶原纤维排列规则，骨陷窝空虚；阿利新蓝染色可见 AECM骨陷窝周边染色呈不同程度蓝绿色；免疫组织化学染色可见 FN和 L N的抗体染色阳性部位在细胞外基质。

Osteoblasts were collected from the rabbit fetus skull by collagenase digestion associated with tissue nubbles cultivation and cultured in vitro.

酶消化-组织块联合培养法体外培养胎兔颅骨成骨细胞：取新西兰大白兔胎兔颅骨骨块，制成2mm 2mm骨块，Ⅰ型胶原酶室温下消化。

Cell sheet transplantation regenerated both new bone and cementum connecting with well-oriented collagen fibers, while only limited bone regeneration was observed in control group where cell sheet transplantationwas eliminated.

结果发现，细胞膜片移植再生出了新骨、牙骨质以及连接二者的排列整齐的胶原纤维，而在没有进行细胞膜片移植的对照组只观察到了有限的骨再生。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。