胶骨

Objective To investigate the expression of type Ⅱ collagen in the articular chondrocyte of osteoarthritis patients and normal human.

目的 通过观察Ⅱ型胶原mRNA在骨关节炎(osteoarthritis，OA)患者关节软骨细胞与正常关节软骨细胞中的表达情况，探讨Ⅱ型胶原在骨关节炎中的作用。

Methods All archival serial celloidin sections of temporal bones are stored in the temporal bone bank of PLA general hospital.

方法所有颞骨火棉胶切片均来自解放军总医院颞骨库，基于颞骨火棉胶切片的DNA提取、PCR扩增、原位杂交、原位PCR和免疫蛋白染色技术被用来研究老年性耳聋的分子病理机制。

OBJECTIVE: Using membrane guided tissue regeneration technique, to observe the healing velocity and quality of repairing rabbit radius segmental defect combined collagen membrane with allogeneic bone, further more, to contrast the result with pure collagen membrane.

目的：利用膜引导组织再生技术，观察胶原膜复合同种骨颗粒修复兔桡骨节段性骨缺损的成骨速度和质量，并与单纯胶原膜进行比较。

Both of the G ranula group and the chondrogenic inductor group can promote the expression of collagen Ⅱ, which is the highest in the G ranula group. The is no expression of collagen Ⅱ in the blank group.

透骨消痛颗粒组﹑软骨诱导剂组均有Ⅱ型胶原表达，而且透骨消痛颗粒组比软骨诱导剂组表达强，空白对照组没有Ⅱ型胶原表达。

Bushen Zhuanggu granules were consisted of deerhorn glue, Chelonia Glue, Leech and Common Yan Rhizome; Xianling Gubao capsule was offered by Guizhou Tongjitang Pharmaceutical Co. Ltd.

补肾壮骨颗粒主要由鹿角胶、龟胶、山药、水蛭等药物组成；仙灵骨葆胶囊由贵州同济堂制药有限公司提供；维甲酸由武汉远城科技发展有限公司提供。

Bushen Zhuanggu granules were consisted of deerhorn glue, Chelonia Glue, Leech and Common Yan Rhizome; Xianling Gubao capsule was offered by Guizhou Tongjitang Pharmaceutical Co.

补肾壮骨颗粒主要由鹿角胶、龟胶、山药、水蛭等药物组成；仙灵骨葆胶囊由贵州同济堂制药有限公司提供；维甲酸由武汉远城科技发展有限公司提供。

In this study, immunohistochemical staining was performed with antibodies against D2-40, S100, pankeratin, epithelial membrane antigen, brachyury, and glial fibrillary acidic protein in 4 cases of chordoid glioma, 6 skeletal myxoid chondrosarcomas, 10 chordoid meningiomas, 16 extraskeletal myxoid chondrosarcoma, 18 chordomas, 22 low-grade chondrosarcomas, and 27 enchondromas.

本研究中，我们给4例脊索样胶质瘤、6例骨的黏液样软骨肉瘤、10脊索样脑膜瘤、16例骨外黏液样软骨肉瘤、18例脊索瘤、22例低级别软骨肉瘤和27例内生性软骨瘤做了D2-40、S100、pankeratin、上皮膜抗原、brachyury和胶质纤维酸性蛋白的免疫组化染色。

This in vivo study was to examine the historical changes of implanted novel chitosan/collagen composite barrier for confirming the clinical feasibility. Four other commercial GTR membranes were chosen for comparison. Among the resorbable GTR membranes, BioMend Extend and Peri-Aid are collagen base, and GORE-TEX OSSEOQUEST is synthesized membrane, while GORE-TEX e-PTFE (Expanded polytetrafluoroethylene) is synthesized but non-resorbable. Beagle dogs were used as animal model. Buccal mucoperiosteal flaps were reflected in the bilateral mandibular premolar and molar areas. Buccal alveolar bone was reduced on 1st、2nd premolar and molar to a level 5 mm apical to the cemento-enemel junction. Root surface was denuded of periodontal ligament and cementum, and notches were placed at the bone level of each root. The tested GTR barriers were implanted in critical bone defect areas. Flaps were coronally positioned and sutured. Two beagle dogs were sacrificed each time as the designed time period after surgery. Histological and histometirc evaluation at 7 days、14 days、28 days、3 months were performed post-operatively to determine the healing response of each treatment modality.

本研究之目的在评估与工研院生医中心合作发展出可吸收性去乙醯几丁聚醣/胶原蛋白复合之组织导引再生膜片，并选择与可吸收性之BioMend Extend 及Peri-Aid 胶原蛋白膜片，和GORE-TEX OSSEOQUEST 合成高分子膜片，及不可吸收之GORE-TEX e-PTFE合成高分子膜片，等其他四种市售商品材料共同对照评估，应用8只年龄为12个月的雄性小猎犬，均分4组(分别为7天、14天、28天、3个月)，为动物活体评估模式，在实验犬之左、右下颚第一、二小臼齿及大臼齿的颊侧区制造骨缺损后，分别植入组织导引再生膜片，依实验设定时间将小猎犬牺牲，取下缺损区骨头，以光学显微镜观察量测其牙垩质再生高度与齿槽骨再生高度之变化，以探讨其组织再生模式及评估新膜片在临床应用之适用性。

Encapsulated psoralen were evaluated by dialyzation using psoralen gel as the control. Changes of liposome????encapsulated psoralen and release rate were detected during a 3????week storage at 4 ℃ for evaluating the stability of the liposomal formulation.

以同浓度的补骨脂素凝胶为对照，用透析法检测补骨脂素脂质体凝胶的体外释药模式，并对其在4 ℃下贮存3周的释药稳定性进行研究。

METHODS: The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition, and cultured in vitro with collagenase digestion. All cells were divided into 3 groups: in the BMP-2 group, cells were cultured with medium containing 0.1 g/L vitamin C, 10 mmol/Lβ-sodium glycerophosphate and 10μg/L BMP-2 for 10 minutes, followed by 4-14 days inoculation with density of 18×104 cells per pore. In the BMP-7 group, cells were cultured with BMP-7 with the same methods as BMP-2 group. The cells were cultured with simple culture medium in the control group.

无菌切取成年新西兰大白兔皮下脂肪，胶原酶消化法体外分离培养脂肪干细胞，分为3组：骨形成蛋白2组加入含0.1 g/L维生素C、10 mmol/L β-甘油磷酸钠、10 μg/L骨形成蛋白2的诱导培养基培育15 min，然后按18×104个细胞/孔接种，再培育4~14 d；骨形成蛋白7组培养方法基本相同，仅将骨形成蛋白2更换为骨形成蛋白7；对照组同法加入单纯培养基进行培育。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。