胶质细胞瘤

Result:The monoclonal antibody H12 against human glioma which the hybridizing cell secretions can consociate specifically with human glioma.

结果：该杂交瘤细胞株分泌抗人脑胶质瘤细胞单克隆抗体H12具有与脑胶质瘤细胞特异性结合的特性。

Purposes: To observe intakes of 〓p-sodium-orthophosphate and 〓P-chromium- orthophosphate at various concentrations by brain gliomas cell; To make thorough inquiry of the inhibition to the SHG44 cell by 〓P-sodium-orthophosphate and 〓P-chromium-orthophosphate at various concentrations; To find out the regularities about the accumulation and elimination in the major organs of rats after inter-brain injection with 〓P-sodium-orthophosphate and 〓P-chromic-orthophosphate; To investigate the therapeutic effects of intert-injection with 〓p-chromium-orthophosphate on the neoplasia transplanted in the brain and hypoderma; To estimate the absorbed dose of the neoplasia body in rats after internal radiation; To study the pathological changes in the rat cells and tissues caused by the radiation exposure from 〓p.

目的：观察人脑胶质瘤SHG44细胞对不同浓度的〓P-磷酸钠和〓P-磷酸铬的摄取；探讨不同浓度〓p-磷酸钠和〓p-磷酸铬对SHG44细胞生长的抑制；揭示〓p-磷酸钠和〓p-磷酸铬在大鼠颅内注射在时全身主要脏器的蓄积与排除规律；观察〓p-磷酸铬大鼠颅内瘤体、皮下瘤体内注射的治疗效果；估算大鼠瘤体组织的吸收剂量；观察〓p照射引起的细胞及组织的病理学改变，为临床脑胶质瘤内照射治疗提供实验依据和吸收剂量计算方法。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

Results: The findings on CT and MRI were as follows: 57 cases of Oligodendroglioma, 15 cases of Ganglioglioma , 5 cases of Dysembryoplastic neuroepithelial tumor , 3 cases of Pleomorphic xanthoastrocytoma .

结果：少枝胶质瘤47例，间变性少枝胶质瘤10例，节细胞胶质瘤15例，胚胎发育不良性神经上皮瘤5例，多形黄色细胞瘤3例。

Methods:Thirty-seven glioma specimens were classified as astrocytoma(25 cases, including 7 fibrillary cases; 6 protoplasmic cases; 12 anaplasfic cases), and glioblastoma( 12 cases,including 4 GBM cases).

37例人脑胶质瘤标本按WHO分类标准(1990)分为：星形细胞瘤(25例，纤维型7例，原浆型6例，间变型12例)，胶质母细胞瘤(12例，包括GBM4例)。

The expression of PCNA mirrors the general rule of cell multiplication and differentiation of glioma.

PCNA的表达反应了脑胶质瘤细胞增殖与分化的一般规律， PTEN表达的降低在脑胶质瘤的发生、发展过程中可能起着非常重要的作用，PCNA和PTEN的联合检测有利于更准确地判断胶质瘤生物行为和评估患者预后。

The expression of PCNA mirrors the general rule of cell multiplication and differentiation of glioma. The decrease of the expression of PTEN may have very important effect on development and growth of glioma.

PCNA的表达反应了脑胶质瘤细胞增殖与分化的一般规律， PTEN表达的降低在脑胶质瘤的发生、发展过程中可能起着非常重要的作用，PCNA和PTEN的联合检测有利于更准确地判断胶质瘤生物行为和评估患者预后。

Results Only DCs cocultured with apoptotic cells can induce special CTLs, while the group of U937 cells and culture cells can not.

结果 与凋亡胶质瘤细胞共培养之DCs可以有效提呈胶质瘤细胞抗原，有强烈的免疫应答，刺激的细胞毒T淋巴细胞特异性杀伤胶质瘤细胞。

TIL of human glioma is of good ability of proliferation in vitro, and this can not be affected significantly by retrovirus-mediated TNF-α gene transduc- tion into it. TIL is suitable as carrier cells for lymphokine gene therapy of human glioma.

1。人脑胶质瘤TIL具有良好的体外增殖能力，逆转录病毒中介TNF-α基因导入人脑胶质瘤TIL后，其体外增殖能力未受明显影响，符合人脑胶质瘤淋巴因子基因治疗运载细胞的要求。

Oncolytic adenovirus H101 could replicate and cause oncolysis effects on glioblastoma U251 cells, it shows greater oncolysis effects and much more H101mRNA expression by enhanced virus tite or prolonged time.

1。溶瘤病毒制剂H101对体外培养的人类胶质母细胞瘤U251存在明显的抑制作用，能够在肿瘤细胞内复制，并最终起到溶瘤作用，并且胶质母细胞瘤U251细胞内病毒的产量随时间的延长而增大。2。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷