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胶质细胞

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With time, this network of collagen and elastin fibers breaks down, and hyaluronic acid molecules and fat cells that create volume are depleted.

随着时间的推移,这个网络的胶原蛋白和弹性蛋白纤维分解,透明质酸分子和脂肪细胞产生量日益枯竭。

The result demonstrates that the capillaries and fibrils in dermis of PSS have marked changes, but less marked in that of LS.

结果表明,真皮层毛细血管的内皮细胞、基底膜和胶原原纤维及其周围的基质均发生明显的变化。

RESULTS: Light microscope showed that the collagen fibers arranged regularly in AECM with blankness of bone lacunas by HE, Mallory-Heidenhain rapid one-step dyeing and that the region around bone lacunas was stained different degrees of blue-green by Alcian blue dyeing.

结果 HE、Mallory- Heidenhain氏快速一步法染色后镜下可见AECM胶原纤维排列规则,骨陷窝空虚;阿利新蓝染色可见 AECM骨陷窝周边染色呈不同程度蓝绿色;免疫组织化学染色可见 FN和 L N的抗体染色阳性部位在细胞外基质。

This research is taking sea perch as the material, and cloning the Syntaxin 1B gene. After extracting RNA from sea perch tissue, we gained the first chain of cDNA by RT-PCR. Then designing a pair of primers according to the sequence of Syntaxin 1B from other organism. Based on the first chain of cDNA cloned from sea perch, the partial sequence of Syntaxin 1B gene is been cloned by using TD-PCR and we received the 751bp fragment at last.

本实验从鲈鱼脑组织中提取总RNA,通过反转录得到cDNA第一条链,根据已克隆出的其它生物的Syntaxin 1B基因序列,设计并合成引物,在妒鱼syntaxin1B基因部分序列克隆与分析 cDNA第一条链的基础上使用降落PCR,进行体外扩增妒鱼 Syntaxin IB基因的部分序列,得到长度为75 lbP的片断,在胶中洗脱下该片段,与pMD 18一T载体链接,转化入感受态细胞,挑取阳性克隆扩大培养,提取质粒,经HindIH和x一gaH两内切酶消化得到75 1 bp的片断,证明链接成功。

Methods TIL in the patients with PHC were separated with combinative enzymatic digestion of type Ⅰ,Ⅳ collagenases,type Ⅴ hyaluronidase and type Ⅰ deoxyribonuclease followed by discontinuous density gradients.Cytotoxicity of TIL to liver cancer cells was detected with MTT method.

采用Ⅰ型和Ⅳ型胶原酶、Ⅴ型透明质酸酶和Ⅰ型DNA酶四联酶消化,应用不连续密度梯度离心分离TIL,采用MTT比色法检测TIL对自体癌细胞及肝癌细胞株的细胞毒。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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