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胶质细胞

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Methods Wistar rats of 6 months old were randomly divided into normal control groupand IR group. Each group had eight rats. Euglycemic insulin clamp technique was used to determine IR and glycose infusion rate was used to IR. Cardiocyte apoptosis was evaluated by TUNEL. Heart weight and body weight were measured to calculate HW/BW. Fine structure of heart and ultramicrostructure of cardiocyte were observed.

6月龄Wistar大鼠,分为正常对照组和IR组,正常血糖胰岛素钳夹技术测定各组大鼠IR,葡萄糖输注速率表示IR情况;测量大鼠心脏重量和体重并计算心脏重量与体重之比;原位末端酶标记法检测各组大鼠心肌细胞凋亡情况;观察心脏微细结构和心肌细胞超微结构;免疫组化检测心脏间质Ⅰ、Ⅲ型胶原水平。

Result: The divided functional recipes of DHZCP could significantly decreased the deposit of lipid and the atherosclerotic plaque area in aorta intima, relieve the histopathological changes of atherosclerosis,and inhibited the proliferation of vascular smooth muscle cells and collagen to reduce pachynsis of vascular intima. The divided functional recipes of DHZCP also reduced IT, IMT and IT/MT and reversed the contractive vascular remodeling.

结果:大黄虫丸各功效组分均能减少家兔主动脉内膜脂质沉积,使AS斑块面积减小( P 。05);抑制血管平滑肌细胞增殖和胶原纤维增生,减轻内膜增厚;降低IT,IMT,IT/IMT比值( P 。05),干预收缩性血管重塑;减轻线粒体肿胀,减少泡沫样细胞形成。

Fig 4 Few of rough endoplasmic reticula, mitochondrid and co llagen can be seen in the cytoplasm of fibroblast, the ribosome of rER is degran ulated, the nucleous is abnormal, there are a lot of granule (maybe the antisens e oligodeoxynucleotide) in the cytoplasm TEM×5200

图4反义核酸组的瘢痕成纤维细胞粗面内质网较少、有脱颗粒现象,线粒体少,胞核畸形,胞内有吞噬颗粒,细胞周围胶原纤维较少TEM×5200

Therefore, collagen metabolism is the center of the extracellular matrix remodeling in intracranial saccular aneurysms, while the essence of the remodeling process is a kind of autoimmunological and inflammatory reaction.

因此,胶原代谢是囊性动脉瘤细胞外基质重塑的核心内容,而重塑过程的实质是一种以细胞免疫为主的免疫炎性反应。

Low reaction of fetus to inflammation, different extracellular matrix, component of collagen fiber and cell factors may take part in scarless healing process. Regulation of gene expression may play an important role in the process. But the concrete mechanism of scarless healing is not clear yet and still needs more researches and elucidations.

胎儿对炎症的低反应性、胎儿不同的细胞外基质、胶原纤维的成分、细胞因子可能也参与了无瘢痕愈合过程、基因表达调控在其过程中可能也起重要作用,但无瘢痕愈合的具体机制还不明了,有待进一步研究阐明。

The ultimate result of chronic HC injury is the accumulation of extracellular matrix with high density type I collagen within the subendothelial space of Disse, culminating in cirrhosis with hepatocellular dysfunction.

慢性HC损伤的最后的结果是在内皮下间隙高密度的1型胶原的细胞外基质堆积,最后为肝细胞的功能障碍、肝硬化。

The infected tissues were changed at the cellular level in morphological structure and physiological and biochemical metabolism, such as cell wall collapse, tylose plugging in air cavities, reduction of glairin and amylum, cell lignification, and brown gum in vessels. The crude toxin produced by F.

受侵染的植物组织在细胞水平上发生一系列形态结构和生理生化代谢的病理变化,包括细胞壁消解、侵填体堵塞气腔、粘胶质减少、淀粉量减少、细胞木质化和黄褐色胶状物堵塞导管等。

Apoptosis of myocytes was observed in the peripheral infarcted area, which appeared as shrinkage of cells and a condensation or marginating of chromatin. Large fragmentations and ladder bands were seen in DNA gel electrophoresis.

梗塞边缘区可见凋亡心肌细胞,其形态表现为细胞皱缩,细胞核染色质聚集成块或边集;DNA凝胶电泳可见大片段或梯形带。

Results The physically and chemically treated SIS was pure with more hollows on the surface and undamaged collagen fibers. The cartilage cells could grow, adhere to and differentiate on the layer of SIS and enter into the hollows, secrete a great deal of extracellular matrices.

结果 经物理和化学处理的SIS纯度高、孔隙多,胶原纤维未受损;软骨细胞在SIS材料上生长、黏附、增殖,并能长入材料的孔隙内,分泌大量的细胞外基质成分。

METHODS: The hUV-MSCs were isolated by the collagenase digestion method. At 1×108/L, hUV-MSCs were incubated in a 24-well culture plate, and then divided into two groups. These in the experimental group were treated with 150 g/L VEGF, whereas those in the control group were left intact.

采用胶原酶消化法分离人脐静脉间充质干细胞,以1×108 L-1的密度接种于24孔培养板,设立2组,实验组加入150 g/L血管内皮细胞生长因子,对照组不加入任何细胞因子。

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