胶质细胞

The adventitia is the outermost layer of the artery and typically consists of a loose matrix of elastin, smooth muscle cells, fibroblasts, and collagen.

动脉外膜是动脉的最外层，典型上，包含了疏松的弹力蛋白基质、平滑肌细胞、纤维母细胞、以及胶原。

MATERIALS: The hircine bone mesenchymal stem cells were in vitro cultured, and resuspended with algin solution to obtain BMSCs-calcium alginate gel.

材料：体外培养并扩增健康成年雄性山羊的骨髓基质干细胞，收集约3×107细胞，加入1 mL藻酸钠溶液重悬形成骨髓基质干细胞-藻酸钙凝胶材料。

Objective To investigate the feasibility of biological fibrin glue/cancellous bone matrix as scaffold for chondrocyte.

目的 探讨生物蛋白胶，松质骨基质作为软骨细胞培养支架的可行性。

RESULTS: After 3 days of operation, the BMP positive cells were found in the matrix of experimental group I.

将自体成骨样细胞即刻种植在胶原包埋的聚羟基乙酸基质材料上，然后将该复合体或单纯基质材料移植到兔颅骨的一侧全层骨缺损区，作为实验侧或实验侧。

In order to study the expression and functional characteristics of dehydrin gene during different growth, and make the polycolonal antibody, using wheat plumelet under the water deficit condition as experiment material, and its total RNA was extracted. The target dehydrin gene through RT-PCR was got, connect to the cloning vector PUCM-T, recombination expression plasmid PET-32a-wzy1-1 was constructed according to recombination of cloned vector PUCM-T-WZY1-1 of wheat dehydrin gene in this experiment.

为研究小麦在不同时期脱水素基因的表达情况和生物学功能及抗体制备，以小麦幼芽为材料，经干旱胁迫处理后，提取总RNA，通过RT-PCR得到小麦类脱水素基因片段(WZY1-1)，再连接至克隆载体PUCM-T，并成功构建重组表达质粒PET-32a-wzy1-1，将阳性重组质粒转化于受体菌BL21(DE3)感受态细胞中，经IPTG诱导表达，进行表达产物的聚丙烯酰胺凝胶电泳检测。

Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

软骨组织工程的材料学研究主要体现在两类基质材料方面的进展，一类为天然基质材料，如胶原、壳聚糖、纤维素等；一类为人工合成可降解材料，如PGA、PLA等，但两者在来源、塑形能力及细胞识别信号等方面存在不足。

Results Incubation of TEC with MAC increased the expression of collagen type Ⅳ(P.05) in comparison with unstimulated cell.Conclusion MAC can i...

结论亚溶破剂量的MAC能使HKC的Ⅳ型胶原表达上调，可能是引起肾小管间质纤维化细胞外基质增多的原因之一。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Transmission electron microscopy demonstrated that fibroblast was full of endoplasmic reticulums that cisternal-like expanded in the rhEGF nanoparticle group, and collagen proteins were synthesized in cavity.

透射电镜下可见rhEGF纳米微球组成纤维细胞胞质内有大量的内质网，扩张成池状，腔内充满了合成的胶原蛋白。

We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast interaction with collagen fibers by stimulating b1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.

我们指出基质修改激素松弛肽通过分泌活性b1-integrin ，从而增加了瘤相关纤维原细胞与胶原纤维的相互作用。这对于基质金属蛋白酶对纤维的重塑很重要。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷