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胶质细胞

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In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

Acellular allogeneic dermal matrix is the dermal replacement of heterogenous human skin in which the cellular elements have been removed by special technique and basement membrane complex have been remained. It becomes a kind of 3D porous acellular dermal scaffold. It supports fibroblast infiltration, neovascularization and epithelialization in the absence of an inflammatory response.

异体脱细胞真皮基质(acellular allogeneic dermal matrix, ADM)来源于正常的异体皮肤组织,经过特殊的理化处理去除真皮中的细胞成分,保留原有的胶原三维结构和完整的基底膜,成为一种多孔的三维膜性支架,其空间网状结构有利于细胞的增殖、毛细血管增生,加速组织的愈合过程,移植后可以迅速被血管化和自体细胞重建。

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞,使之转化为成骨细胞;用RT-PCR检验成骨细胞;不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基,作用于成骨细胞,并分析生长曲线,分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

Then test of film cell growth by high sugar, LPS, high sugar plus LPS was done with the result showing that FANG KIDNEY capsule can restrain the growth of film cell and composition of external base, it can be concluded that it has obvious effect in preventing the hardening of kidney small cell.

为进一步探讨糖肾胶囊的作用机制,还采用国内外经典细胞培养方法,成功地培养出肾小球系膜细胞,在此基础上进行高糖、LPS、及高糖加LPS诱导细胞膜增生,结果发现糖肾胶囊能抑制系膜细胞增生及细胞外基质胶原合成,证明其对防止肾小球硬化有明显作用。

The part of the embryonic mesoderm, consisting of loosely packed, unspecialized cells set in a gelatinous ground substance, from which connective tissue, bone, cartilage, and the circulatory and lymphatic systems develop.

间叶细胞胚胎的中胚层部分,由生长于胶状基质的、结构松散的、无特定功能的细胞组成,这些细胞将会发育成结缔组织、骨、软骨、血液及淋巴系统

Human/rat homology collagen type I siRNA plasmid not only direct repress collagen type I gene expression in rat inesangial cells, but also have the same inhibition action of TGF-β1 induced overexpression of collagen type Ⅰ.

本实验构建的人鼠同源Ⅰ型胶原siRNA质粒,在大鼠系膜细胞内不仅能够直接抑制Ⅰ型胶原的基因表达,而且对TGF-β1诱导的I型胶原表达增加同样具有较好的抑制作用。

The part of the embryonic mesoderm, consisting of loosely packed , unspecialized cells set in a

间叶细胞胚胎的中胚层部分,由生长于胶状基质的、结构松散的、无特定功能的细胞组成,这些细胞将会发育成结缔组织、骨、软骨、血液及淋巴系统

Results In model group, alveolitis increases (much inflammatory cell infiltration), pulmonary interstitial fibroblast increases.

结果]模型组7d时肺泡腔内大量炎性细胞浸润,可见少量成纤维细胞,Ⅰ型细胞受损,Ⅱ型细胞明显增生;28d时肺泡炎减轻,肺间质和胸膜层胶原纤维明显增多。

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry. The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.

取健康的猪甲状腺细胞培养24h后,加入不同浓度的碘化钾,观察细胞的形态改变;琼脂糖凝胶电泳、流式细胞术分析细胞DNA的降解;MDA法测定细胞膜的脂质过氧化程度。

Results Microscopically, different degrees of hepatocytic degenerative changes and hyperplasic fibrous tissue and typical false lobule formation could be seen. Timm's staining result showed uneven distributed black granular deposits in the hepatocytes. No specific PAS staining was observed. Utrastructurally, the mitochondria were increased in volume and dramatically different in shape. The number of lysomes were increased.

结果 光镜下肝细胞表现为不同程度的退行性变,胶原纤维增生以及典型假小叶形成;Timm's染色阳性,发现不均匀分布黑色颗粒或团块样物质沉积;PAS染色则普遍缺乏特异性染色;超微结构显示线粒体形态多样,体积增大,溶酶体增多,粗面内质网管腔扩张,附着核糖体的脱颗粒,以及胞质水肿,质膜溶解。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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