胶质细胞

A small portion of BrdU and NeuN double labeled cells could be detected in the cortex at the early stage of development, and in the striatum and CA of the hippocampus in all groups. The majority of BrdU labeled cells were neuroglia, and the number of neuroglia cells decreased dramatically with brain maturation. Neurogenesis is the major cytogenesis in the dentate gyrus.

皮层在发育早期以及纹状体和海马CA区在发育各期检测到极少数为新生神经元细胞，多数分化为胶质细胞；海马齿状回以神经元细胞再生为主；胶质细胞的再生随脑组织发育的成熟而逐渐减少。

The positive expression of Slit2 located in the cytoplast on oligodendrocyte and astrocyte.The positive cells were found at 3d after spinal cord injury, reached peak on 7d after injury, declining after 14d. The change of Slit2 was correlative with the rehabilitation and regeneration of the axon on the forepart period.

免疫组织化学检测：Slit2蛋白定位于星型胶质细胞和少突胶质细胞的胞浆，呈棕黄色染色，于脊髓损伤后第3 d出现，阳性细胞逐渐增多，于脊髓损伤后第7 d，Slit2阳性表达的细胞数达到高峰并趋于稳定，伤后第14 d开始逐渐下降，呈一过性增高。

It has been found that ectogenic GDNF has nervous protective effec,but ectogenic GDNF cannot enter blood brain barrier.

传统观点认为，星形胶质细胞是神经系统的支持细胞，构成神经组织的网架而起支持作用，与神经元的解剖位置关系紧密，参与中枢神经系统的免疫反应，参与构成血脑屏障，目前研究发现，脑缺血时星形胶质细胞异常活跃，通过合成多种蛋白质、细胞因子和神经营养因子等对神经元起保护作用，对缺血性脑损伤的发展和转归具有重要意义。

Results (1) In the controls and the dilated intestine segment of the HD,CAD-positive ganglionic cells were observed in myenteric and submucosal plexus.

结果 （1）在正常对照组与HD扩张段组肠壁肌间和黏膜下层可见CAD只对神经丛中神经节细胞阳性表达，对神经纤维与神经胶质细胞均不表达。S-100染色与CAD染色相反，神经纤维与神经胶质细胞均阳性表达，神经节细胞阴性表达，表现为阳性神经丛中细胞状&空白区&。

Result bFGF immunoreactivity was found in neuron and gli al cell in corte x,hippocampus habenular nucleus,and found in macrophage surrounding hematoma; Ln immunoreactivity was found in neurons,glial cells and blood vessles in front a l cortex,hippocampus,thalamus hypothalamus.

结果 脑出血后脑内bFGF主要在扣带皮质、海马、缰核及其他区域表达，阳性细胞骨折主要有神经哮喘元、神经哮喘胶质、吞噬细胞骨折、室管膜细胞骨折、伸长细胞骨折等；Ln不仅在大脑皮质、海马、缰核、下丘脑等区域的神经哮喘元表达，且在这些区域的神经哮喘胶质细胞骨折也表达，血管也有阳性反应。

Hours after operation, most nerve cells disappeared and the glial cells were degenerated. There were many mono-nucleus cells infiltrating in transplantation group while a few in control group. One week after operation, tissues in the infarction zone were liquescent with many monocaryons and lymphocytes infiltrating around them in control group.

术后48 h神经细胞大片消失，胶质细胞变性，自体骨髓干细胞移植组有大量单个核细胞浸润，对照组相对较少。1周后对照组梗死区脑组织液化变软，坏死灶周围可见较多的单核和淋巴细胞浸润；自体骨髓干细胞移植组大鼠部分坏死灶被新生毛细血管和胶质细胞增生修复，炎性细胞浸润不明显。

Microglia is always considered to be a kind of most representative immune cell in the central nervous system.

小胶质细胞一向被认为是中枢神经系统中最有代表性的免疫细胞，生理情况下小胶质细胞占脑细胞总数的10％左右，其生理功能以吞噬清除细胞残骸为主，保证脑内内环境的稳定。

Oligodenderocyte and oligodendroglioma cells in vitro were able to secret beta-trace protein and explained why the concentration of β-tp in CSF was higher than that in serum, it was suggested that the level of β-tp released depended on both of cell member and type.

胶质细胞和人脑胶质瘤细胞能分泌β-tp，细胞分泌β-tp水平的高低由细胞类型及细胞的多寡决定。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

Methods The astrocytes were isolated from cerebral cortex of neonatal rats which are born within three days, cultivating by DMEM media added with 15% fetal calf serum and depurating by swinging and passaging, to simulate nulli-nutrition model by substituting medium for PBS buffer solution 3h then applying methylprednisolone and recnutrition immediately, we will go on culturing for three days, verificating astrocytes by immunofluorescent technique; detecting activity of AST; determining GFAP of change by PCR.

采用出生3d内的SD大鼠的新生鼠的大脑制成细胞悬浊液后，应用含15%胎牛血清的DMEM培养液中培养，通过摇床和逐渐传代纯化星形胶质细胞，通过PBS缓冲液代替培养基模拟缺营养模型3h后即刻应用甲基强的松龙(10umol/l)，然后继续培养3d，免疫荧光技术鉴定星形胶质细胞；MTT法检测细胞的增殖活性和凋亡；PCR技术测定细胞GFAP表达的变化。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷