胶质细胞

Between P14 and P21,CIAPIN1 immunoreaction in the brain,heart and liverbecame much lower. However,between P21 and P28,CIAPIN1 immunoreactionin the heart,brain,liver and skeletal muscle became much lower,while with thekidney development,CIAPIN1 immunoreaction in the kidney became higher. Invarious tissues from adult mouse,CIAPIN1 immunoreaction could be seen incardiac muscle cell,brain,hepatocyte,epithelium of renal tubule,skeletal muscle,lung tissue,gastric mucosa and gland,acinus lienalis.2. Distribution of CIAPIN1 in normal fetal and adult human tissuesTo reveal the possible physiological role of CIAPIN1,we examined theexpression and distribution of CIAPIN1 in fetal and adult human tissues usingimmunohistochemistry. We found that CIAPIN1 was ubiquitously distributed infetal and adult tissues,and was localized in both the cytoplasm and the nucleus.

然而，在3个月大的成年鼠中，CIAPIN1阳性反应物在心、脑、肝和肾小管中的表达强度要低于P28小鼠；但CIAPIN1阳性反应物在成年鼠骨骼肌中较P28小鼠高。2、CIAPIN1蛋白在人5个月胚胎及成人多器官组织内的表达在人5月胚胎多器官组织中，CIAPIN1阳性反应物见于心脏、胆囊单层柱状上皮和粘膜、结肠粘膜、小肠粘膜和绒毛、肝脏、直肠腺体、胃粘膜、肾上腺束状带、甲状腺滤泡、脾索、胸腺小叶间隔、皮肤真皮层和汗腺、睾丸白膜和间质、脑组织内神经元和神经胶质、肺小支气管和肺泡、骨骼肌、肾脏皮髓质和肾小管、子宫内膜、胰腺腺泡和胰岛、卵巢、输卵管粘膜等绝大多数组织细胞。

The combination of epidermal growth factor, basic fibroblast growth factor and insulin-like growth factor can effectively promote the directional differentiation of BMSCs into oligodendrocytes.

碱性成纤维细胞生长因子、表皮生长因子与胰岛素样生长因子联合应用能够有效促进骨髓间充质干细胞向少突胶质样细胞定向分化。

Transduction of TNF-α gene into TIL of human glioma by retroviral vectors and study of its biological activity in vitro. The development of retroviral vectors for TNF-α gene transfer and preparation of carrier cells-TIL have al- lowed transducing marked gene NeoR and purpose gene TNF-α into TIL cells respectively by use of monoclone tilter virus PLC-2 and PLJC-5. Detect the proliferation of TIL, expression of TNF-α and the anti-tumor activity of glio- ma TJ8510 cells in vitro.

在TNF-α逆转录病毒转移系统的建立及运载细胞TIL制备的基础上，利用构建的单克隆高滴度病毒细胞株PLC-2和PLJC-5将标记基因NcoR和目的基因TNF-α分别导入到TIL细胞中；对TNF-α基因转导后的TIL细胞进行细胞的增殖情况、TNF-α表达水平及对人脑胶质瘤TJ8510细胞的体外抗瘤活性的检测。

Previous studies have shown thatlymphocytes from patients with malig-nant disease, when encountering abasic protein fraction isolated fromhuman glioma, display a slowingeffect to macrophage electrophoreticmobility.

对用人脑胶质瘤碱性蛋白免疫的38只豚鼠和23只对照组豚鼠进行了巨噬细胞电泳试验，其巨噬细胞电泳减缓率免疫组为6.13±1.06%，对照组为0.96±0.90%；经统计学处理，两组间有显著区别，表明GBP能激发豚鼠的细胞免疫反应性。

Results ① The percentage of positive TPOAb and TGAb were 91.4% and 74.4%, respectively in all the AIT patients. 47.6% of the patients had TSH levels within normal range (0.3～5mu/L).② All of the slides had different grades of lymphocytic infiltration. 49.3% had germinal center, 32.8% had Askanazy cells, 26.9% had plasma cells, 22.4% had colloid, and 9% had multinuclear giant cells.③ Lymphocytic infiltration was divided into four degrees. The levels of TSH and TPOAb increased significantly in the extremely heavy lymphocytic infiltration grade than in the others (P.05). There was no relationship between serologic markers and other cytopathologic features.

结果 ①82例患者中TPOAb、TGAb的阳性率分别为91.4%和74.4%，约有半数(47.6%)TSH值位于正常值范围内(0.3～5mu/L)；②所有患者细胞病理学均存在淋巴细胞不同程度的浸润，49.3%可见生发中心，32.8%可见嗜酸性变细胞，26.9%可见浆细胞，22.4%可见胶质，9%可见多核巨细胞；③将淋巴细胞浸润程度分级，分别与TSH、TPOAb及TGAb进行相关性分析，发现仅淋巴细胞极重度浸润者其血清TSH、TPOAb水平较其他组显著升高(P.05)，其他细胞病理学改变与血清学指标之间未发现显著相关性。

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

近年来脑胶质瘤的基因治疗发展迅速，应运而生的方法有自杀基因、免疫基因、多药耐药基因以及抗血管生成基因等，其中自杀基因被认为是最有前景的基因治疗方法，它又称病毒介导的酶／药物前体疗法，是利用转基因技术将哺乳动物细胞中所不含有的自杀基因转入到哺乳动物肿瘤细胞中，该基因表达的产物可将无毒的药物前体转化为毒性药物，从而选择性杀伤该肿瘤细胞，常用的自杀基因有单纯疱疹病毒-胸苷激酶基因和大肠杆菌胞嘧啶脱氨酶基因，前者催化无毒性抗病毒核苷类似物如丙氧鸟苷、无环鸟苷等成为单磷酸核苷衍生物，然后在内源性细胞激酶作用下转化为具有明显毒性的三磷酸核苷，作为DNA合成链的终止剂和DNA合成酶的抑制剂，干扰细胞DNA的合成；后者编码的胞嘧啶脱氨酶可催化5-氟胞嘧啶（5-FC）脱氨成为5-氟尿嘧啶（5-FU），然后代谢为有毒性的5-氟尿嘧啶-5′三磷酸（5-FUTP）和5-氟-2′脱氧尿嘧啶-5′磷酸（5-FdUTP），5-FUTP通过与UTP竞争性结合而抑制mRNA和tRNA的合成，5-FdUTP则作用于胸苷合成酶，导致TMP衰竭而阻止DNA的合成，最终诱导肿瘤细胞凋亡。

In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.

Wstar大鼠术后1周的暗适应视杆反应，a波潜伏期缩短o叼。05）；术后2周的暗适应视杆反应，a波潜伏期延长o功刀5）：术后1周的暗适应最大反应，b波潜伏期缩短o功刀5卜＊CS大鼠术后4周的暗适应视杆反应和明适应反应，a波振幅增大o功刀匀；术后4周的暗适应视杆反应，a波潜伏期缩短o＜0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡，导致FERG的a、b波振幅明显降低；视网膜下腔仍存在感光细胞外节盘膜碎屑，外丛状层缺乏感光细胞的突触末端，同时Muler细胞出现反应性胶质纤维增生。

This study was to investigate the impact of all-trans retinoic acid on the gene expression profile of glioblastoma cell line SHG-44, and to provide basic data for further research on gene therapy for human astrocytoma.

本研究旨在检测全反式维甲酸(all-trans retinoic acid， ATRA)处理后胶质瘤SHG-44细胞的基因表达谱改变，为进一步研究胶质瘤的基因治疗奠定基础。

LRIG3 could be a targeted protein for gene therapy of glioma.

LRIG3可通过EGFR信号系统影响胶质瘤细胞的生长，有望成为胶质瘤分子治疗的靶点。

Histologic examination revealed pleomorphic cellular proliferation with xanthomatous, multi and mono nucleated giant cells.

免疫组织化学染色显示瘤细胞胶质纤维酸性蛋白阳性，网质纤维染色显示单个瘤细胞间着色。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。