胶质细胞

Results The hind limb function of the injured rats recovered at different degrees, the most extent recovery occurred during the time site 1~2 week, recovery continued from 2 to 3 week and, BBB was up to 12 at the end of the third week, but there was no significance recovery during 3~4 week. the astrocyte caudal to the injury plane began hyperplasy and hypertrophy; astrocyte in which GFAP in expression was positive the gray matter increased obviously from 3 days to 14 days after hemisection. The expression of MBP is same as that of GFAP.

结果 伤后后肢均有不同程度的恢复，1~2周时恢复幅度最大，2~3周时后肢运动功能继续恢复，3周时BBB评分最高达12分，3~4周运动功能无显著性恢复，损伤后1 d损伤远端3~6 mm处GFAP阳性星形胶质细胞开始增生肥大，3~4 d灰质中星形胶质细胞明显增多，2周时达到高峰，损伤近端3~6 mm处少突胶质细胞的增生反应过程与星形胶质细胞相似。

The GFAP is a specific marker of astrocyte, its expression is more higher in the activity astrocyte, and finally the GFAP become the main composition of scar formations. The S -100 is a kind of acidity, dissolubilites, low molecular quantity and calcium hydronium conjugated protein, and it is mainly existed in neuroglial cell and schwann cell. It can promote the growth of axon, glial hyper-plasia and nerve divide and calcium's stability inside of cell, thus regulatin g the shape and metabolism of astrocyte . The quantity of S -100 protein and the degree of ischemia have direct proportion .

胶质纤维酸性蛋白是星形胶质细胞的特异性标记物，在活性星形胶质细胞中GFAP的表达相对更高，且最后GFAP成为胶质疤痕的主要成份。S-100蛋白是一种酸性、可溶性、低分子量的钙离子结合蛋白，主要存在于胶质细胞和雪旺细胞中，它可促进轴突生长、胶质增生、神经分化和细胞内钙的稳定，从而调节星形胶质细胞的形态和代谢。S-100蛋白与缺血的程度是成正比的。

Neuroectoderm; neural crest; neural plate; neural tube; neuraxis; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；神经管；轴索；成神经细胞；神经干细胞；neuroprogenitor细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；神经节发生；胚胎发生；轴突发生；视网膜发生；神经胶质发生；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管缺陷；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发育阶段

Glia; gliogenesis; glioma; glial cells; central nervous system; CNS; glial progenitor cells; glial fibrillary acidic protein; ependymocytes; ependoma; cytokeratins; vimentin; tanycytes; ion transport; lining cells; cerebrospinal fluid; CSF; ventricular system; floor plate; axon guidance; choroidal cells; choroid plexus epithelium; Chiari malformations; congenital aqueductal stenosis; neuroblast migration disorders; gap junctions

神经胶质；gliogenesis；神经胶质瘤；胶质细胞；中枢神经系统；CNS；神经胶质祖细胞；胶质原纤维酸性蛋白；室管膜细胞；ependoma；细胞角蛋白；波形蛋白；tanycytes；离子转运；衬细胞；脑脊髓液；CSF；脑室系统；底板；；脉络膜细胞；脉络丛上皮；恰里畸形；先天性导水管狭窄；成神经细胞迁徙扰乱；缺隙连接点

Results (1)Astrocytes and oligodendrocytes in mixed culture appeared better growth and differentiation than the respectively purified neuroglial, especially for the oligodendrocytes.(2) There were many pseudopod process among the astrocytes, which appeared centripetal growth mode.(3) The passaged astrocytes kept good abilities of division and proliferation and completely fused after 1 week of growth, and the purity was over 95%.

结果 (1)混合培养的星形胶质细胞和少突胶质细胞生长分化状况均优于纯化后的生长，以少突胶质细胞表现更为明显；(2)星形胶质细胞呈向心性生长，细胞相邻的接触面有较多的伪足；(3)传代培养的星形胶质细胞保持较强的分裂增殖能力，可在1周左右生长至完全融合，其纯度可达95%以上。

After cocultured with reactive astrocytes, olfactory ensheathing cells can promote the proliferation of reactive astrocytes,and reduce the astrocyte gliosis and expression of inhibitory cytokines.

嗅鞘细胞作用于活化后的星形胶质细胞以后，能够促进星形胶质细胞的分裂增殖，同时能够减少星形胶质细胞胶质化，降低抑制性细胞因子的表达。

Results: ApoD was observed positively expressed in type2 astrocytes and oligodendrocytes from O2A lineage, while it was not expressed in type1 astrocytes from T1A lineage. Accordingly, we can verify the phenomenon found in the study of T1A and T2A gene different expression profile that ApoD mRNA was highly expressed in T2A and lowly expressed in T1A. Conclusion: It can be concluded that lipid metabolism related genes such as ApoD play important roles in the generation and differentiation of O2A lineage, which closely correlate with internal mechanism of lipid metabolism in the brain and myelogenesis.［Key words］astrocyte(T1A, T2A); oligodendrocyte; O2A lineage; apolipoproteinApoD

我们已采用基因芯片技术在mRNA水平研究两型星形胶质细胞的基因表达谱差异，发现ApoD在2型星形胶质细胞中差异高表达，故本文拟用激光共聚焦双重免疫荧光标记技术进一步在蛋白质水平观察ApoD在体外分化成熟的两型星形胶质细胞以及少突胶质细胞中的表达情况，以期为我们深入研究ApoD等脂代谢相关基因在脑内脂质代谢和神经髓鞘发育机制中所起的作用提供有用的实验依据，依此探讨胶质细胞发生和分化的信号机制。

Results: the findings on ct and mri were as follows: 57 cases of oligodendroglioma, 15 cases of ganglioglioma , 5 cases of dysembryoplastic neuroepithelial tumor , 3 cases of pleomorphic xanthoastrocytoma .conclusion:the images on ct and mri of cerebra peripheral tumor occur some characters , so ct and mri were certain worth on the diagnose and differentiate diagnose of cerebra peripheral tumor.

结果：少枝胶质瘤47例，间变性少枝胶质瘤10例，节细胞胶质瘤15例，胚胎发育不良性神经上皮瘤5例，多形黄色细胞瘤3例。结论：脑浅表肿瘤存在影像特征，ct及mri对其诊断有一定的价值。少枝胶质细胞瘤；节细胞肿瘤；胚胎发育不良性神经上皮瘤；多形性黄色星形细胞瘤；ct；mri

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用：主要表现为：(1)对受损神经元具有直接的保护作用，可提高受损神经元的活性和存活率，降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量；(2)对受损的星形胶质细胞也有直接的保护作用，可提高其活性、存活率以及培养液蛋白质的含量；(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力；(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达，降低受损神经元对bax和caspase-3的表达；(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元，因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用，统计学上具有显著性差异。

Results Tag was found in all of 8 ependymomas, 2 choroid plexus papillomas, 2 human glioma cell lines, 90% of pituitary adenomas (9/10), 73% of astrocytomas (11/15), 70% of meningiomas (7/10), 50% of glioblastomas multiforme (4/8), and 33% of medulloblastomas (2/6). None of the 8 normal brain tissues were positive for Tag, nor were 5 oligodendrogliomas and 1 pineocytoma.

结果 Tag在8例室管膜瘤、2例脉络丛乳头状瘤及2株人脑胶质瘤细胞系中全部表达；垂体腺瘤Tag阳性率为90%(9/10)，星形胶质细胞瘤73%(11/15)，脑膜瘤70%(7/10)，多形性胶质母细胞瘤50%(4/8)，髓母细胞瘤33%(2/6)；5例少枝胶质细胞瘤、1例松果体瘤及8例正常人脑组织无Tag表达。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。