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Organic scale from asphaltene precipitation is one of main causes of formation damage,Therefore to take some effective prevention and remove measures is very necessary.

在原油开采过程中,原油中沥青质、胶质等重有机质会在岩石表面沉积,形成有机垢,造成不同程度的油气层损害,并且某些损害是永久性的、不可逆的,因此采取有效的预防措施至关重要。

Finally, two short-path distillation processes and one falling film evaporator are applied in the regeneration of waste lubricating oil. In the test, the diesel oil, gasoline and lubricating oil are distilled respectively, while the gelatinoid materials, asphaltene and carbon residue remain in the draft. The regenerative lubricating oil accords with the standards of base oil. And the recovery efficiency is up to 75 percent. The technology used in the test doesnt comprise processes like pickling and alkali neutralization, which would cause secondary pollution like acid draff and sulfur dioxide and so on. As a result of this, our technology is friendly to the environment.

最后,利用一级薄膜蒸发和两级短程蒸馏工艺对废润滑油进行了再生回收工业应用试验,将柴油、汽油等轻油以及润滑油馏分分别蒸馏出来,而大部分胶质、沥青质、碳垢存在于残渣中,试验得到的再生润滑油符合基础油的标准,回收率达到75%,达到废润滑油再生的目的;同时再生过程中没有酸洗、碱中和等工序,不产生酸渣、二氧化硫等二次污染,是一种清洁环保的废润滑油再生工艺。

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P<0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P <0.001.

结果:构建了带有HSV-tk基因的反转录病毒载体GINaTK,应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317,成为产病毒细胞PA317TK,用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞,命名为C6TK细胞,对GCV和ACV的敏感性分别高于亲本450倍和10倍;成功地建立了大鼠脑胶质瘤模型,并证实转染细胞C6TK的成瘤效应未改变,存活期约为15天;而经ACV治疗后,含有C6TK细胞的肿瘤生长明显被抑制,大鼠生存期延长为57.8±8.07天,尤其是采用PA317TK细胞混和治疗组和原位治疗组,肿瘤基本消失,大鼠生存期显著延长,混和治疗组存活120天以上,原位治疗组存活至71.4±36.1天。t检验,P值均小于0.001。

In the present study,we intend to establish a new model of traumatic brain-steminjury,and to study the pathological changes of neuron,axon,astrocyte,plasmaproteins,blood vessel endothelia,synapse,neurotransmitter and cytokines in rat'sand human injured brain-stem,using routing histochemistry,immunohistochemistry,in situ hybridization,LSCM and computerized image analysis.

综合现有文献,本研究拟建立新的脑干损伤动物模型,并采用常规病理组化、免疫组化、原位杂交、LSCM以及计算机图像分析等技术,对动物及人体脑干组织中的神经元、星形胶质细胞、血浆蛋白及血管内皮细胞,以及轴索、突触、神经递质及细胞因子等进行研究,并对脑干损伤的致死性病变进行初步定位,以确立对脑干损伤的鉴定和推断脑干损伤时间有意义的客观指标。

But the latent period for reaching the platform above the water in Morris Water Maze of the Mn-exposed group was not different from that of control group. There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高;(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高;(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

Bone marrow stromal cells are cells got rid of hemopoietic stem cells in marrow cell of mature individual, which is approved to be multi-differentiation potential including transforming into neural stem cell, and differentiation into neurs and neuroglial cell.

骨髓基质细胞(Bone marrow stromal cells,BMSCs)是成体骨髓细胞中除造血干细胞之外的另一类骨髓干细胞,近年来的研究证明其具有多向分化的潜能,包括向神经干细胞转化,并可进一步分化为神经元和神经胶质细胞。

We have researched distribution of TGF β and its receptor in various age group and discs of various degeneration grade, found that positive ratio of TGF β and its receptor is higher in below 40 year old group and discs of MRI Ⅰ and Ⅱ grade, mainly occur in fibrous cartilagous cells of fibrous annulus and nucleus pulpous; In group of 40-60 years old and grade Ⅱ and Ⅲ discs, TGF β decreased and TGF β R decreased not as much as TGF β; In above 60 years old group, TGF β and its receptor almost disappear.

生长因子包括TGF-β对细胞功能有重要调节作用,它是一种相对分子质量为2.5×10〓的二聚体多肽,几乎参与了哺乳动物所有细胞的病理,生理过程,尤其是在细胞外基质的产生及调控方面意义重大,且TGF-β已被公认为是与胶原代谢关系最密切的细胞因子。胡有谷、陈宏等,应用杂交技术检测了TGF-β对椎间盘Ⅰ、Ⅲ型胶原MRA的调节作用。

Not mainly the ageing of resins and asphaltenes but that of saturates and aromatics cause the ageing of asphalt.

沥青热氧老化的原因主要是饱和分和芳香分的氧化,而不是胶质和沥青质的氧化。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。