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The white carbon black formed good thermal conductivity network with the molecular chain of the rubber, and the dispersion of the white carbon black in the natural rubber matrix was improved when the surface of the white carbon black was treated with Si-69, and the thermal diffusivity of the rubber was improved greatly.

白炭黑在Si-69的表面改性作用下,能与胶料分子链形成较好的导热网络结构,使其在天然橡胶基质中的分散程度得到改善,从而使胶料的热扩散系数得以提高。

The gene regulating sequence of human collagen type Ⅰ is located at 5'end and first intron. The carriers of reporting gene was made, and the function of promoter, enhancer and inhibiter was examined in this study. On the basis of above work, MBP ectopic expression carriers were further built. The result laid a foundation for investigating the feasibility, and validity to specially express MBP gene of human nervous system, in tendon fibroblast.

人Ⅰ型胶原基因调节序列位于5'端和第一内含子,本研究构建了带报告基因的载体并检测了其启动子,增强子和减弱子的功能,以此为基础,进一步构建了MBP异位表达载体,为探索在神经系统少突胶质细胞特异表达的人脑髓鞘碱性蛋白MBP基因转至肌腱成纤维细胞表达的可行性和有效性奠定了基础。

RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ,Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes.

结果:种植于不同基质的纤维环细胞形态学观察无明显差别;在两种基质上的纤维环细胞甲苯胺蓝染色无差异;Ⅰ,Ⅱ型胶原及聚集蛋白聚糖mRNA及细胞膜整合素β1的表达亦无明显差异;纤维环细胞可以黏附于硅橡胶膜上生长。

Infected oligodendrocytes or type 2 PML cells with oval enlarged showing replacement of granular chromatin by basophilic or amphophilic, homogenized, ground-glass like viral inclusion bodies different from eosinophilic viral inclusion bodies of Cowdry type

感染的少突胶质细胞,或2型PML细胞,伴卵圆形拉长的核,显示颗粒状染色质变成嗜碱性或双嗜性、均质、毛玻璃样病毒包涵体(与Cowdry型的嗜酸性病毒包涵体不同)。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml,经percoll液分离后密度梯度离心,〓/ml密度接种培养,观察原代细胞的贴壁、增殖状况,细胞长满瓶底后进行传代培养;传至第三代细胞,重新消化后以500转/分钟轻度离心5分钟,〓/ml接种,改用化学限定培养基代替完全培养基培养,倒置显微镜观察细胞生长情况及形态变化,甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖,免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成,RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml,经 percoll液分离后密度梯度离心,10'砌l密度接种培养,观察原代细胞的贴壁、增殖状况,细胞长满瓶底后进行传代培养;传至第三代细胞,重新消化后以500转/分钟轻度离心 5分钟,10V加 l接种,改用化学限定培养基代替完全培养基培养,倒置显微镜观察细胞生长情况及形态变化,甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM)中的蛋白多糖,免疫细胞化学染色检测ECM中*胶原的蛋白合成,RT干CR鉴定诱导细胞*胶原mRNA的表达。

At present, the study on NSCs has been gotten delightfully. But how to induce the NSCs to the specific neuron or specific neuroglial cell efficiently need to be studied further.

目前神经于细胞研究已经取得了可喜的进展,但如何高效地诱导神经干细胞分化为待定递质类型的神经元或特定类型的神经胶质细胞有待进一步研究。

The higher resin and asphalt the crude oil contains, the more demulsifier is needed.

胶质和沥青质含量高的原油对破乳剂的需要量也大。

BACKGROUND: Basic fibroblast growth factor can accelerate the bone marrow mesenchymal stem cells proliferation and differentiation into nerve cells, which is considered as a mitogen of glial cells.

背景:碱性成纤维细胞生长因子可以促进骨髓间充质干细胞的增殖和向神经细胞方向分化,并被认为是胶质细胞的分裂原。

Chitosansilica hybrid beadsbearing with metal ion chelating group was prepared by solgel method and microemulsion technique. The condition for the preparation of CSHB, as well as its structure and morphology were investigated.

采用溶胶凝胶及微乳液技术制备了以壳聚糖硅基杂化材料为骨架并带有金属离子螯合官能团的球形基质,并对该基质的制备条件及结构形貌进行了研究与表征。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。