胶质型的

MMP9 and collagen Ⅳ both expressed in basement membrane.

MMP9和Ⅳ型胶原主要表达于细胞外基质中的基底膜上。

Objective To study the differentiation of human glioma cells BT-325 induced by sodium butyrate in vitro.

目的 研究丁酸钠体外诱导人脑多型性胶质母细胞瘤细胞系 BT-325细胞的分化。

Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Results: GINaTK retroviral vector containing HSV-tk was constructed and transduced into PA317 retrovirus packaging cell by lipofectin (named PA317TK). Rat glioma cell C6 was infected by the deficiency recombinant retrovirus (named C6TK). The sensitivity of C6TK cells to GCV or ACV increased 450 or 10 times than that of C6 cells respectively. Rat C6 glioma model was successfully built, and SD rats inoculated intracerebrally C6TK cells had normal gliomas. The average survival periods of the rats were about 15 days. However, after treated with ACV, the growth of C6TK tumors obviously regressed, and the survival periods extended to 57.8±8.1 days, especially in rats injected with mixed PA317TK and C6 cells or in situ PA317TK cells, which the tumors nearly disappeared after ACV administration and the survival periods extended to over 120 or 71.4±36.1 days respectively, P ＜0.001.

结果：构建了带有HSV-tk基因的反转录病毒载体GINaTK，应用脂质体转移方法将GINaTK导入反转录病毒包装细胞PA317，成为产病毒细胞PA317TK，用带有HSV-tk基因的复制缺陷型反转录病毒感染C6细胞，命名为C6TK细胞，对GCV和ACV的敏感性分别高于亲本450倍和10倍；成功地建立了大鼠脑胶质瘤模型，并证实转染细胞C6TK的成瘤效应未改变，存活期约为15天；而经ACV治疗后，含有C6TK细胞的肿瘤生长明显被抑制，大鼠生存期延长为57.8±8.07天，尤其是采用PA317TK细胞混和治疗组和原位治疗组，肿瘤基本消失，大鼠生存期显著延长，混和治疗组存活120天以上，原位治疗组存活至71.4±36.1天。t检验，P值均小于0.001。

Objective To study the toxic effects induced by rotenone on primary cultured astroglia.

目的 观察鱼藤酮对体外培养星型胶质细胞毒性作用的特点。

RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ,Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes.

结果：种植于不同基质的纤维环细胞形态学观察无明显差别；在两种基质上的纤维环细胞甲苯胺蓝染色无差异；Ⅰ，Ⅱ型胶原及聚集蛋白聚糖mRNA及细胞膜整合素β1的表达亦无明显差异；纤维环细胞可以黏附于硅橡胶膜上生长。

The granule could significantly ameliorate mesenchymal deposit of collagenⅠ,Ⅲ,Ⅳ and α-SMA due to ureter ligation in rats with UUO, lower hyperexpression of TGF-β1 mRNA and TIMP-1 mRNA after renal injury, decrease the number of cells infiltrating into renal mesenchyma.

在单侧输尿管结扎大鼠中补肾生血颗粒剂能明显改善输尿管结扎所致的Ⅰ、Ⅲ、Ⅳ型胶原和α-SMA在肾间质中的沉积，降低肾脏损伤后亢进的TGF-β1 mRNA和TIMP-1 mRNA的水平；减少肾间质中浸润的〓T淋巴细胞数目。

We have researched distribution of TGF β and its receptor in various age group and discs of various degeneration grade, found that positive ratio of TGF β and its receptor is higher in below 40 year old group and discs of MRI Ⅰ and Ⅱ grade, mainly occur in fibrous cartilagous cells of fibrous annulus and nucleus pulpous; In group of 40-60 years old and grade Ⅱ and Ⅲ discs, TGF β decreased and TGF β R decreased not as much as TGF β; In above 60 years old group, TGF β and its receptor almost disappear.

生长因子包括TGF-β对细胞功能有重要调节作用，它是一种相对分子质量为2.5×10〓的二聚体多肽，几乎参与了哺乳动物所有细胞的病理，生理过程，尤其是在细胞外基质的产生及调控方面意义重大，且TGF-β已被公认为是与胶原代谢关系最密切的细胞因子。胡有谷、陈宏等，应用杂交技术检测了TGF-β对椎间盘Ⅰ、Ⅲ型胶原MRA的调节作用。

Results Incubation of TEC with MAC increased the expression of collagen type Ⅳ(P.05) in comparison with unstimulated cell.Conclusion MAC can i...

结论亚溶破剂量的MAC能使HKC的Ⅳ型胶原表达上调，可能是引起肾小管间质纤维化细胞外基质增多的原因之一。

Methods: rabbit bone marrow mesenchymal stem cells were purified, and then cultured in inducing medium containing transforming growth factor-β1; mtt assay was employed to evaluate the proliferative activity of induced cells; immunohistochemical assay was used to detect the expression of type ⅱ collagen; induced cells were transplanted into the knee of rabbit, and x-ray photography was employed to observe the repair and formation of cartilage.

纯化兔骨髓间充质干细胞；用含转化生长因子β1的诱导液培养骨髓间充质干细胞；以mtt测定诱导细胞的增殖活性；免疫组化检测ⅱ型胶原蛋白的表达；诱导后的细胞移植于白兔膝关节内，x线摄片观察软骨修复形成情况。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失