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The purpose of this study was to investigate the CDK1 expression levels in gliomas and the effects of CDK1 knockdown on phenotype of glioma cells.

目的探讨周期蛋白依赖性激酶1(CDK1)在胶质瘤组织中的表达及其沉默对胶质瘤细胞恶性表型的影响。

He type of ossein in group B was mainly type Ⅰ collagen while that in group A type Ⅱ and it exhibited as a process of fiber formation in group C. The reason for why TGF-β〓 can promote ossification induced by BMP is that can promote the differentiation and proliferation of mesenchymal cells to supply more target cells for BMP, promote the differentiation and proliferation of mature osteocytes and chondrocytes, inhibit metalloproteinase to decrease the destruction of bone tissue and promote calcification of bone matrix.

GF-〓能促进BMP骨诱导作用主要因其能促进间充质细胞的分裂增殖为BMP提供丰富的靶细胞、促进成熟骨及软骨细胞的分裂增殖、促进骨细胞产生Ⅰ型胶原而抑制软骨细胞产生Ⅱ型胶原,抑制金属蛋白酶的活性以减少组织破坏、促进基质钙化等作用而使骨生成能力增加,提示它和BMP具有协同作用。

Based on the short gel-time features of ordinary cement sodium-silicate and alkali-activated salic cementitious materials, an ideal structure model of high performance sodium-silicate suspension two-shot grouting material was established. An experiment was undertaken to verify the solidification value of Na~+ and water-erosion resistance of a grouting material combined with cement and metakaolin-sodium silicate. This paper put forward a compact structure with a dominant ingredient of-Si-Al-H zeolite gel formed on later age and a minor part of C-S-H formed on early age, which composed of sodium-silicate, substances that could release Ca~(2+) spontaneously and stably or by the aid of sodium-silicate, and active salic substances.

结合普通水泥-水玻璃快速胶凝和碱激发硅铝质胶凝材料的特点,本文建立了高性能水玻璃悬浊型双液灌浆材料的理想结构模型,并通过水泥复合偏高岭土-水玻璃灌浆材料的Na~+固化性能和抗水溶蚀性能进行了试验验证,提出其组成结构应是以-Si-Al-H类沸石凝胶物质为主、C-S-H为辅的复合共存密实结构,其组成要素为水玻璃、能自发稳定地或在水玻璃作用下迅速释放出Ca~(2+)的物质、活性硅铝质材料。

Dose-dependent toxic effects on primary cultured astroglia could be induced by being exposed to rotenone, but astroglia base higher tolerance to the same dose of rotenone compared with neurons.

鱼藤酮对体外培养星型胶质细胞可以产生明显的毒性损伤作用,但与神经元比较,星型胶质细胞对相同剂量的鱼藤酮染毒具有更强的耐受能力。

MTT assay and morphological observation were used to evaluate the toxicity of rotenone on astroglia,cell proliferation and the expression of CX43 mRNA were detected by growth curve analysis and RTPCR.

观察染毒星型胶质细胞形态,检测细胞活力,通过生长曲线分析鱼藤酮对星型胶质细胞增殖的影响以及RTPCR检测缝隙连接蛋白(connexin43,CX43)mRNA表达。

Dosedependent toxic effects on primary cultured astroglia could be induced by being exposed to rotenone,but astroglia have higher tolerance to the same dose of rotenone compared with neurons.

鱼藤酮对体外培养星型胶质细胞可以产生明显的毒性损伤作用,但与神经元比较,星型胶质细胞对相同剂量的鱼藤酮染毒具有更强的耐受能力。

Dosedependent toxic effects on primary cultured astroglia could be induced by being exposed to rotenone,but astroglia have higher tolerance to the same dose of rotenone compared with neurons.

鱼藤酮对体外培养星型胶质细胞可以产生明显的毒性损伤功能,但和神经元比较,星型胶质细胞对相同剂量的鱼藤酮染毒具有更强的耐受能力。

MTT assay arid morphological observation were used to evaluate the toxicity of rotenone on astroglia, cell proliferation and the expression of CX43 mRNA were detected by growth curve analysis and RT-PCR.

观察染毒星型胶质细胞形态,检测细胞活力,通过生长曲线分析鱼藤酮对星型胶质细胞增殖的影响以及RT-PCR检测缝隙连接蛋白(connexin43, CX43)mRNA表达。

The objectives of the study were to investigate the characteristics and influencing factors of myocardial interstitial collagen of right ventricle in tetralogy of Fallot.Tissues of the infundibular septum from 22 patients with TOF were studied and 8 patients without cardiovascular disease and collagen system disease were used as control.Collagen were determined by biochemical methods.

为了解法洛四联症肥大右室心肌间质Ⅰ、Ⅲ型胶原改建的特点及其影响因素,用生化等方法定量分析了22例法洛四联症病人和8例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ、Ⅲ型胶原比值,以及法洛四联症病人的血氧饱和度等影响因素。

Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.

构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体,将其导入逆转录病毒包装细胞PA317,通过G418抗性筛选,得到携带目的基因的包装细胞并鉴定;应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞,观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响;建立C6胶质瘤大鼠脑内移植动物模型,将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中,观察其治疗作用,并初步探讨其机制。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。