胶质型的

The liver and kidney were partly removed and fixed with 4 % paraform phosphate buffer. The remnant of the liver was stored in liquid nitrogen and -80℃. 4.examination: Serum: ALT, AST, BUN, Scr in serum were measured by biochemical analyses and MDA,SOD by spectrophotometric methods and HA , LN, C-IV by radioimmunological methods.

指标检测：血清：全自动生化分析仪检测血清中谷丙转氨酶、谷草转氨酶、尿素氮、血肌酐的含量，放射免疫的方法检测血清透明质酸、层粘连蛋白、IV型胶原，分光光度计法检测血清中超氧化物歧化酶活性及丙二醛的变化。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The ultimate result of chronic HC injury is the accumulation of extracellular matrix with high density type I collagen within the subendothelial space of Disse, culminating in cirrhosis with hepatocellular dysfunction.

慢性HC损伤的最后的结果是在内皮下间隙高密度的1型胶原的细胞外基质堆积，最后为肝细胞的功能障碍、肝硬化。

BACKGROUND: Based on documents abroad and home, both alginic acid and carbomer are used to prepare gel matrix of lithium carbonate tablets; furthermore, hydroxypropyl methyl cellulose, a kind of water-soluble skeletal material, can be used as controlled release matrix of lithium carbonate tablets.

摘要背景：目前国内外文献报道的碳酸锂缓释片多采用海藻酸、卡波姆等制备凝胶型骨架片，同样作为水溶性骨架材料的羟丙基甲基纤维素，也可作为碳酸锂的缓释基质。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失