胶质
- 与 胶质 相关的网络例句 [注:此内容来源于网络,仅供参考]
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Methods DCs were prepared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4. Apoptosis of glioma cells were induced with γ-radiation. We design these experiment groups including (1) coculture of DCs and apoptotic glioma cells and T cells,(2) coculture of DCs and U937 cells and T cells,(3) coculture of DCs and cultured glioma cell and T cells,(4) coculture of Des and T cell.
用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡,将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养,同时设计不同类型肿瘤细胞(U937及培养胶质瘤细胞)作对照,分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。
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After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.
实验方法:1、培养原代的人RPE细胞,经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后,通过AO/PI染色技术确定培养细胞的存活率,描记其生长曲线,第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后,通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后,采用MTT法观察其对细胞的增殖力的作用;通过流式细胞仪观察其对细胞周期的影响;通过扫描电镜观察其对细胞形态的影响;通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用;采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率,评价其对细胞间通讯功能的影响;通过制作RPE细胞损伤模型,观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质,应用等电聚焦电泳和SDS-PAGE双向电泳技术,银染显示分离出的蛋白质斑点,经凝胶图像分析软件对两个样本进行胶图分析,寻找差异蛋白点。
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Cyte including protoplasmic astrocyte and fibrous astrocyte, oligodendrocyte, microglia, and epen
细胞和纤维型星形胶质细胞,少突胶质细胞、小胶质细胞和室管膜细胞;在周围神经系统中
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A case is made that areas of chronic demyelination arise because of concurrent loss of oligodendrocyte progenitor cells and oligodendrocytes and that because of the slow rate of repopulation that occurs in old individuals the recruited OPCs are not exposed to the acute inflammatory environment required to generate remyelinating oligodendrocytes.
一种说法是慢性脱髓鞘的区域产生系因少突胶质原始细胞和少突胶质细胞并存,而且因老年个体中缓慢的再生率,补充的OPCs没有暴露于产生髓鞘再生少突胶质细胞需要的急性炎症的环境。
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By culturing human brain microvascular endothelial cells and glioma cells separatedly and simutaneously ,and then analyzing their pre- and post-cultune FasL levels, we study the effect of the grouth of glioma cells upon FasL level of human brain microvascular endothelial cells.
我们通过将人脑毛细血管内皮细胞与胶质瘤细胞分别培养及共同培养,对培养前后人脑毛细血管内皮细胞与胶质瘤细胞FasL表达水平的分析,来探讨胶质瘤细胞的生长对人脑毛细血管内皮细胞FasL表达的影响。
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Methods At first, retrovirus vectors encoding IFN-γ or IL-4 gene were constructed. They were transfected into PA317 packaging cells by lipofectamine, PA317IFN-γ and PA317IL-4 cells were obtained. C6 glioma cells were infected with replication deficiency retrovius containing IFN-γ or IL-4 gene, cell morphology、cell growth curve and cloning efficiency assay were examined. The intracranial C6 glioma animal model was established in immunocompetent Wistar rats. PA317IFN-γ and PA317IL-4 cells were stereotactically implanted into the tumor areas alone or together. The survival of tumor bearing rats were examined, tumor volumes were measured and immunohistochemical analyses of CD4〓 and CD8〓 T lymphocyte infiltration were performed.
构建和鉴定携带IL-4或IFN-γ基因的逆转录病毒载体,将其导入逆转录病毒包装细胞PA317,通过G418抗性筛选,得到携带目的基因的包装细胞并鉴定;应用携带IL-4或IFN-γ基因的复制缺陷型逆转录病毒感染C6胶质瘤细胞,观察对C6胶质瘤细胞形态、细胞生长曲线和克隆形成率的影响;建立C6胶质瘤大鼠脑内移植动物模型,将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中,观察其治疗作用,并初步探讨其机制。
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The expression of PCNA mirrors the general rule of cell multiplication and differentiation of glioma.
PCNA的表达反应了脑胶质瘤细胞增殖与分化的一般规律, PTEN表达的降低在脑胶质瘤的发生、发展过程中可能起着非常重要的作用,PCNA和PTEN的联合检测有利于更准确地判断胶质瘤生物行为和评估患者预后。
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The expression of PCNA mirrors the general rule of cell multiplication and differentiation of glioma. The decrease of the expression of PTEN may have very important effect on development and growth of glioma.
PCNA的表达反应了脑胶质瘤细胞增殖与分化的一般规律, PTEN表达的降低在脑胶质瘤的发生、发展过程中可能起着非常重要的作用,PCNA和PTEN的联合检测有利于更准确地判断胶质瘤生物行为和评估患者预后。
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Human hair keratin ; spinal cord injury ; rat ; tissue engineering ; tissue-engineered spinal cord ; connector ; morphology ; praxiology ; neuron ; axis-cylinder ; nerve fiber ; myelin sheath ; astrocyte ; oligodendrocyte ; microglia ; ependymal cell ; neural stem cells ; macrophage ; multinuclear giant cell ; lysosomal enzyme ; ubiquitin system ; suppression subtractive hybridization ; gene express ; CytohromeoxidaseⅢ; amino acid transporter system A2(Ata2); Peroxiredoxins 5(prd 5); cysteine proteinase inhibitor cystatin c
国家自然科学基金;广东省自然科学基金人发角蛋白;脊髓损伤;大鼠;组织工程学;组织工程化脊髓;桥接物;形态学;行为学;神经元;轴突;神经纤维;髓鞘;星形胶质细胞;少突胶质细胞;小胶质细胞;室管膜细胞;神经干细胞;巨噬细胞;多核巨细胞;溶酶体酶;泛素肽酶系;抑制消减杂交;基因表达;细胞色素氧化酶Ⅲ;氨基酸转运蛋白系统A2;抗过氧化物蛋白5;半胱氨酸蛋白酶抑制剂
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Results: We found that carbachol decreased the number of microglia even in the presence of GM-CSF in dose-dependent manner, with the most effective concentration of 10μM.
结果:我们在培养的小胶质细胞系发现,卡巴胆碱显著减少小胶质细胞的数量,并能有效抑制粒细胞巨噬细胞集落刺激因子诱导的小胶质细胞数量增多,并存在剂量依赖效应,10μ M浓度效果最为显著。
- 推荐网络例句
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The split between the two groups can hardly be papered over.
这两个团体间的分歧难以掩饰。
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This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.
这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。
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The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.
聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力