胶质

The glioma's invasive tissue is the recurring source of glioma in clinical treatment, even after resecting glioma.

在胶质瘤治疗的过程中，即使肿瘤组织自身被切除，浸润组织则是胶质瘤复发的根源。

They were all right-handed.12 cases using conventional surgery were the control group(eight cases of glioma,3 cases of meningioma,1 case of brain metastases),eight cases of men,four cases of women;the average of 42 years old. The other patients were the experimental group(six cases of glioma,2 cases of meningioma, 2 cases of brain metastases,cavernous angioma 1 case),seven cases of men,four cases of women,the average of 45 years old.

其中12例设为常规手术对照组(胶质瘤8例，脑膜瘤3例，脑转移瘤1例)，男性8例、女性4例，平均年龄42岁，11例设为实验组(胶质瘤6例，脑膜瘤2例，脑转移瘤2例，海绵状血管瘤1例)，男性7例、女性4例，平均年龄45岁。

Aerobes can decompose kenaf gums thoroughly into carbon dioxide and water with oxygen and generate a large amount of ATP. However, anaerobes can not decompose the kenaf gums thoroughly and generate much less ATP.

好氧微生物在有氧的条件下能将胶质彻底分解为二氧化碳和水并产生大量的ATP；而厌氧微生物在厌氧的条件下对胶质的分解不彻底，产生的ATP要少得多。

Methods: The number of microglia treated with different doses of carbachol and GM-CSF was determined by MTT assay.

培养的小胶质细胞系，加入不同浓度的卡巴胆碱或卡巴胆碱与粒细胞巨噬细胞集落刺激因子(Granulocyte-Macrophage Colony-Stimulating Factor， GM-CSF)，用MTT方法检测细胞活性，观察对小胶质细胞数量的影响。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

Methods The astrocytes were isolated from cerebral cortex of neonatal rats which are born within three days, cultivating by DMEM media added with 15% fetal calf serum and depurating by swinging and passaging, to simulate nulli-nutrition model by substituting medium for PBS buffer solution 3h then applying methylprednisolone and recnutrition immediately, we will go on culturing for three days, verificating astrocytes by immunofluorescent technique; detecting activity of AST; determining GFAP of change by PCR.

采用出生3d内的SD大鼠的新生鼠的大脑制成细胞悬浊液后，应用含15%胎牛血清的DMEM培养液中培养，通过摇床和逐渐传代纯化星形胶质细胞，通过PBS缓冲液代替培养基模拟缺营养模型3h后即刻应用甲基强的松龙(10umol/l)，然后继续培养3d，免疫荧光技术鉴定星形胶质细胞；MTT法检测细胞的增殖活性和凋亡；PCR技术测定细胞GFAP表达的变化。

After treated in 4% paraformaldehyde,we applied Hematoxylin-Eosin and Nissl staining to exhibit the structure of the retinae and ganglion cells respectively,and also we used immunohistochemical ABC method to display the distribution of GFAP-immunoreactive cells that were specifically classified as the astrocytes.

E 染色以显示视网膜结构，Nissl染色显示神经节细胞，免疫组织化学ABC法染色以显示星形胶质细胞特征性标志物胶质纤维酸性蛋白的阳性反应细胞的分布。

Objective To investiga te the ability of dendritic cells ac quiring antigen from apoptotic glioma cells and inducing CTLs activati on.

目的 观察树突状细胞从凋亡脑胶质瘤细胞获取抗原后，体外诱导抗肿瘤免疫应答及对胶质瘤细胞的特异性免疫杀伤效果。

GFAP overexpessed but NOS neuron decreased, distorted and pale. The glia in SM-DB complex of ONR hypertrophied, but NOS neuron no degeneration. The increased GFAP immunoreactivity and the morphometric and cytological changes in SM-DB complex astrocytes may reflect a sustained upregulation of cellular activity with the insults of neurons, resulting in hypertrophy of glial perikarya and cell processes.

老年减退组星形胶质细胞的细胞体密度较老年正常组和青年组明显增加（P 在GFAP免疫细胞化学和NOS组织化学双重染色的切片上，老年减退大鼠SM-DB星形胶质细胞明显肥大，突起粗壮弯曲，GFAP超量表达，而NOS阳性神经元数量减少，胞体变形，突起减少，染色深度下降。

Oncolytic adenovirus H101 could replicate and cause oncolysis effects on glioblastoma U251 cells, it shows greater oncolysis effects and much more H101mRNA expression by enhanced virus tite or prolonged time.

1。溶瘤病毒制剂H101对体外培养的人类胶质母细胞瘤U251存在明显的抑制作用，能够在肿瘤细胞内复制，并最终起到溶瘤作用，并且胶质母细胞瘤U251细胞内病毒的产量随时间的延长而增大。2。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。