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The level of ADAR gene expression was expressed as ratio expression rate of ADAR gene /β-actin acc...

ADAR3在正常人脑胶质细胞及苯乙酸处理前后的胶质瘤细胞中,均未见表达。结论ADAR2 mRNA水平高表达,可能与高恶性度胶质瘤的发生有关。

Rat in Group B was set an air bladder which was empty and not perfused water. Water of 0.6 ml, 0.8 ml, 1.0 ml, 1.2 ml, 1.4 ml and 1.5 ml was perfused respectively into the air bladders which were set in the colon of rats in group C, D, E, F, G and H. Then the changes of ethology of the rats were recorded in interval of an hour.

应用疼痛行为学的观察方法,观察1h各组动物的行为学改变,并采用免疫荧光化学的方法,观察大鼠DCN中神经元被激活的标志c-fos及胶质细胞的胶质原纤维酸性蛋白(glial fibrillary acidic protein, GFAP)和小胶质细胞特异性补体OX42的表达情况,空白对照组是在疼痛高峰点(1.2 ml)处死取材。

Methods 50 SD rats of C6 brain glioma into the caudate nucleus by stereotactic methods were established.

目的 建立稳定可靠的大鼠C6脑神经胶质瘤模型,并运用1.5 T MRI观察脑胶质瘤瘤体生长的变化规律,为进行脑胶质瘤的在体实验研究提供基础。

Of or relating to or having the properties of a colloid.

胶质的,有关胶质的,或有胶质的特性的。

Nowadays, as more and more attention are paid to glia, which is considered to play an important role in epileptic pathogenesis,In this study, we chose fluoroacetate, a reversible neuroglia metabolic inhibitor as well as a rodenticide that can induce epileptic seizures, to set up a new epileptic animal model by acting on neuroglia to induce seizures.

目前常用的急性化学致痫模型其致痫机制均是作用于神经元,通过对CNS递质的作用来诱发癫痫。虽然,随着胶质细胞在癫痫的发病机制中的作用日益受到关注,但由于缺乏作用于胶质细胞的癫痫模型,使研究胶质细胞在癫痫发病机制中的作用及其与神经元的关系带来了障碍。

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞,用G〓选择压力筛选,细胞免疫荧光检测表明,pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白,这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

Objectives: To study the relationship between matrix metalloproteinases and their inhibitors and the invasion and metastasis of human glioma, as well as the relationship between the expression of MMP-2 and TIMP-2 in human primary and recrudescent glioma and the grade of malignancy.

目的:探讨基质金属蛋白酶类与胶质细胞瘤浸润性生长之间的关系及其在胶质瘤复发中的作用,以及基质金属蛋白酶及其抑制因子(MMP-2、TIMP-2)的阳性表达与胶质细胞瘤病理分级及预后的关系。

Kangdai Ⅰ has protective function to the damaged neurons and astrocytes: Main results:(1) It has direct protective function to the damaged neurons. It can increase the activity and survival rate, decrease the mortality and the transudation rate of LDH in cultured medium and the strong positive cell count of NOS expression of injured neurons.(2) It has also directly protective function to the damaged astrocytes. It can increase the activity and survival rate and protein content in conditioned medium.(3) It can strengthen the ability of BDNF, GDNF, bFGF, HSP and IL-6expression in damaged astrocytes.(4) It can also strengthen obviously the expressions of NSE, bFGF-receptor and bc1-2, lower the expression of bax and caspase-3.(5) It can indirectly protect and restore the damaged neurons by astrocytes. Because the effect of ACMK (ACM interfered by Kangdai Ⅰ) is stronger than ACM+K (ACM associated with Kangdai Ⅰ).

抗呆Ⅰ号对受损的神经元和星形胶质细胞均具有保护作用:主要表现为:(1)对受损神经元具有直接的保护作用,可提高受损神经元的活性和存活率,降低细胞培养液LDH的漏出率、细胞死亡率和NOS染色强阳性细胞的表达量;(2)对受损的星形胶质细胞也有直接的保护作用,可提高其活性、存活率以及培养液蛋白质的含量;(3)能增强受损星形胶质细胞分泌BDNF、GDNF、bFGF、HSP和IL-6的能力;(4)可明显增强受损神经元对NSE、bFGF的受体和bc1-2的表达,降低受损神经元对bax和caspase-3的表达;(5)抗呆Ⅰ号可通过星形胶质细胞间接地保护和修复受损的神经元,因为在多数实验组中经抗呆Ⅰ号作用的ACM的作用远大于ACM与抗呆Ⅰ号联合应用的作用,统计学上具有显著性差异。

The cokes are come from aromatics, colloids, asphaltene and Carbenes which are polycondensated and dehydrogenated. The processing of coking in the system are as follows: aromatics-→colloids-→asphaltene-→Carbenes-→cokes.(4) Coking can be avoided in the plasma reactor by feeding coal powder of 40~60 mesh (30%) and coal powder of less than 140 mesh (70%).

3结焦主要发生在等离子体反应较高温区,由煤中的芳香分、胶质、沥青质和碳青质缩聚脱氢形成;装置结焦形成的大体途径为:芳香分-→胶质-→沥青质-→碳青质-→焦质。

Results Tag was found in all of 8 ependymomas, 2 choroid plexus papillomas, 2 human glioma cell lines, 90% of pituitary adenomas (9/10), 73% of astrocytomas (11/15), 70% of meningiomas (7/10), 50% of glioblastomas multiforme (4/8), and 33% of medulloblastomas (2/6). None of the 8 normal brain tissues were positive for Tag, nor were 5 oligodendrogliomas and 1 pineocytoma.

结果 Tag在8例室管膜瘤、2例脉络丛乳头状瘤及2株人脑胶质瘤细胞系中全部表达;垂体腺瘤Tag阳性率为90%(9/10),星形胶质细胞瘤73%(11/15),脑膜瘤70%(7/10),多形性胶质母细胞瘤50%(4/8),髓母细胞瘤33%(2/6);5例少枝胶质细胞瘤、1例松果体瘤及8例正常人脑组织无Tag表达。

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