胶糖

It was shown by the results that when the cellulose dosage was 0.15FPU/g substrate, combined with the concentrations of CTAB, SDS, Tween 80 or rhamnolipid of 1 cmc in organic solvent mediae, the maximum yields of reducing sugar were obtained,respectively.

结果表明，纤维素酶用量为0.15FPU/g底物时，CTAB、SDS、Tween 80和鼠李糖脂浓度均为1cmc时，有机相体系形成逆胶束，此体系下酶解过程糖产率最高，其中添加鼠李糖脂可使还原糖产率达到198.03mg/g底物。

And a glycochain IbGpl-OL-LbGp5-OL has been found out by the way of Pronase E or β elimination reaction to dissociate the glycochain from the molecules of glycoconjugate and through the separation and purification of it by gel column chromatography, and ion exchange ohromatography and then its repetitive unit construction has been tested by the ways and means of full hybridization and partial acid hydrolisis, methylate analysis, Gc, Gc/Ms, iH and 1/3 CNMR.

通过链霉蛋白酶E或β-消去反应等方法，将糖链从糖缀合物分子上解离下来，经凝胶柱层析或离子交换柱层析进行分离、纯化得到相应的糖链LbGp1-OL～LbGp5-OL。

In the course of separation, the molecular size of XOS was the dominant factor governing the separation in this system, and there was no specific absorption between polyacrylamide gel and XOS.

分离低聚木糖过程中，分子量是影响分离的主要因素，木糖至木六糖和聚丙烯酰胺凝胶之间不存在非特异性吸附现象。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Fluorophore-assisted carbohydrate electrophoresis was used to analyse pectic oligosaccharides. The influence of different factors on the separation of oligosaccharides by FACE was investigated.

荧光辅助糖电泳是首先用荧光衍生化试剂对糖类分子的还原端进行衍生化标记，然后在一定浓度的聚丙烯酰胺凝胶上进行分离的分析方法，可同时分析中性糖与酸性糖。

According to the result showed at 280nm and at 490nm,in the comparison of whether protein absorption top and sugar quantity top overlapped,glycoproteins would be detected preparatorily,and as a result,tubes in two distinct areas had glycoproteins by this method. Proteins were precipitated with trichloroaceticacid and with cold acetone,and glycoprotein was determined from SDS-gel.

再从各收集管的收集液中，用三氯乙酸沉淀蛋白法、冷丙酮沉淀蛋白法相结合浓缩、制备蛋白样品，进行SDS—PAGE，对SDS胶进行PAS糖链染色鉴定糖蛋白，并从茶树叶分级蛋白中准确地鉴定出两个区域的收集管中含有多种糖蛋白。

Yin Yang is strong plastic-based technologies, and tertiary institutions at home and abroad to conduct joint research, development Salim sugar gum-gum, so that we rubber-based product quality and quality will always be in the forefront of the world.

银洋胶基技术力量雄厚，并与国内外大专院校开展联合研究、研发胶姆糖胶基，从而使我们胶基产品的档次和质量始终处于世界前列。

Other types of carbohydrates found in food are the condensed n-acetylated amino sugars of mucopolysaccharides, glycoproteins, and chitin; the condense deoxy sugars of gum, mucilages, and nucleotides; glcosides （sugars condensed with nonsugars）; glucosinolates; cyclitols; and reductone, l-ascorbic acid.

在食物中发现它种碳水化合物还有：粘多糖、糖蛋白、壳多糖之类的缩聚n-乙酰氨基糖；植物胶、粘浆、核苷酸之类的缩合脱氧糖；糖苷；芥子油苷；环多糖；l-抗坏血酸。

Other types of carbohydrates found in food are the conde ed N-acetylated amino sugars of mucopolysaccharides, glycoprotei , and chitin; the conde e deoxy sugars of gum, mucilages, and nucleotides; glcosides; glucosinolates; cyclitols; and reductone, L-ascorbic acid.

在食物中发现它种碳水化合物还有：粘多糖、糖蛋白、壳多糖之类的缩聚N-乙酰氨基糖；植物胶、粘浆、核苷酸之类的缩合脱氧糖；糖苷；芥子油苷；环多糖；L-抗坏血酸。

Hard candy is one white sugar, starch-based material syrup taste of a hard, brittle candy; Hard candy is candy sandwich containing rolls of hard candy; White sugar are creamed candy, starch syrup, oil and dairy products mainly made of materials, protein not below 1.5% fat not less than 3.0%, has a special flavor and creamed Coke flavor candy; gel candy is edible glue, white sugar and starch syrup material mainly made of soft texture of candy; the surface is polished candy Bright solid candy; gum is white sugar candy and plastic-based material can be made of materials or blowing chewing candy; inflatable of sugar candy is within the finer uniform bubble candy; Pressed candy after granulation, bonding, forming the suppression of candy.

其中硬质糖果是以白砂糖、淀粉糖浆为主料的一类口感硬、脆的糖果；硬质夹心糖果是糖果中含有馅心的硬质糖果；乳脂糖果是以白砂糖、淀粉糖浆、油脂和乳制品为主料制成的，蛋白质不低于1.5%，脂肪不低于3.0%，具有特殊乳脂香味和焦香味的糖果；凝胶糖果是以食用胶、白砂糖和淀粉糖浆为主料制成的质地柔软的糖果；抛光糖果是表面光亮坚实的糖果；胶基糖果是用白砂糖和胶基物质为主料制成的可咀嚼或可吹泡的糖果；充气糖果是糖体内部有细密、均匀气泡的糖果；压片糖果是经过造粒、黏合、压制成型的糖果。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。