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Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

采集人体外周血标本15例,分成三份,分别作为新鲜血组、-80℃冻存血组和-20℃冻存血组标本,用TRI Reagent和TRI Reagent-BD两种不同的试剂提取RNA,以RT-PCR方法扩增β-Actin基因,经1.5%琼脂糖凝胶电泳,溴化乙锭染色,紫外透射仪下观察分析。

Methods One hundred thirty PCOS women and one hundred seventy five normal women as controls were enrolled in this study. The genotypes were screened by polymerase chain reaction-On/off switch and the product was isolated by electrophoresis on a 2.5% agarose gel containing ethidium bromide and visualized using an ultraviolet transilluminator.

应用On/off Switch-聚合酶链反应及琼脂糖凝胶电泳分离技术分析130例多囊卵巢综合征患者及175例对照组妇女TNF-α基因rs3179060C/A多态,比较rs3179060C/A两组等位基因及基因型与PCOS及其高雄激素之间的关系。

Methods The genomic DNA extracted from the 2 trematodes was amplified with random amplified polymorphic DNA and then was carried on agarose gel electrophoresis.

应用随机扩增多态性DNA(Random amplified polymorphic DNA,RAPD)技术对斯氏肺吸虫和华支睾吸虫的基因组多态DNA进行扩增,扩增产物经琼脂糖凝胶电泳后分析结果。

Foodstuff: soya protein, agglomerative starch, lees, triticin, wheat starch and so on.

食品:大豆蛋白、胶凝淀粉、酒糟、小麦糖、小麦淀粉等。

Soya protein, agglomerative starch, less, triticin, wheat starch and so on.

食品类:大豆蛋白、胶凝淀粉、酒糟、、小麦糖、小麦淀粉等。

Briefly, glycopeptides generated from in-gel tryptic digestion were enriched by HILIC and divided into two fractions.

简而言之,首先使用HILIC柱料将N-连接糖肽从胰酶胶内消化的样品中分离出来,并且将其分成两份。

Methods Polymerase chain reaction was used to detect HPV 16 DNA and point mutation at condon 12 of Ha-ras oncogene.

应用多聚酶链反应技术扩增HPV16 DNA和Ha-ras癌基因相关片段,分别采用2%琼脂糖凝胶电泳和限制性片段长度多态性分析,检测口腔癌中的HPV16 DNA和Ha-ras癌基因第12位密码子的点突变。

Separation properties of xylooligosaccharides on polyacrylamide gel Bio-Gel P-2 with FPLC system with degassed high purified water as eluant was investigated.

低聚木糖的分离方法包括凝胶过滤层析、离子交换层析、吸附层析等层析分离技术以及膜分离技术和生物分离技术[1]。

Extracted DNA by CTAB, in proportion to the additions of PVP and β-mercap toethanol to the extraction buffer were capable of preventing browning from taking place in the extraction, Tris-Phenol (Stabilized with 0.1M Tris, pH 8.0)/Chloroform/isoamyl alcohol were used to remove protein and impurity in the second extraction, during crude DNA used to Isopropanol DNA and deterged by DNA detergent, without using RNase remove RNA, we detected the quality and concentration of DNA respectively for normal leaves and drought stress leaves by Agarose gel electrophoresis of genomic DNA and ultraviolet spectrometry photo.

用20%聚乙二醇(polyethylene glycol,PEG6000)模拟干旱,采取CTAB提取缓冲液,按一定比例加入PVP和β-巯基乙醇,用Tris平衡酚-氯仿-异戊醇除去蛋白质及杂质,在粗提时用异丙醇沉淀DNA,并用DNA洗液(10mmol/L醋酸铵,75%乙醇)洗涤DNA,在纯化时用乙醇沉淀DNA,且没有加入RNase去除RNA的方法,分别提取2个品种正常和干旱胁迫的苹果属叶片总DNA,并对提取的DNA进行琼脂糖凝胶电泳和紫外分光光度计检测。

Emodin induced the nuclei of K562 with anomalistic and fragmentized for 96 hours. Agarose gel electrophoresis of DNA revealed a typical DNA ladder map of apoptosis.

大黄素作用96h后,荧光显微镜下可见细胞核不规则,核碎裂呈新月形改变,DNA琼脂糖凝胶电泳显示,基因组DNA显现典型的凋亡细胞的梯状图谱。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。